Molecular targets and specific preventive therapy of anticancer drug-induced ovarian failure
| Project/Area Number |
18591808
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
MINAMI Sawako Wakayama Medical University, 医学部, Assistant Professor (90219692)
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| Co-Investigator(Kenkyū-buntansha) |
KOKAWA Katsuji Wakayama Medical University, 医学部, Assistant Professor (80254548)
UTSUNOMIYA Hirotoshi Wakayama Medical University, 医学部, Assistant Professor (60264876)
TANAKA Tetsuji Wakayama Medical University, 医学部, Associated Professor (80275255)
UMESAKI Naohiko Wakayama Medical University, 医学部, Professor (20106339)
田中 和東 和歌山県立医科大学, 医学部, 助手 (00372863)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Anticancer drug / Granulosa cell / apoptosis / CPT-11 / GnRH agonist / GnRH agonist |
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| Research Abstract |
The animal center of our university had been contaminated with mouse hepatitis virus from 2006 to 2007. Therefore, all the experimental animals including our mice were killed at first, and then the center was disinfected. However, 6 months after the disinfection, virus was found out again in the animal center. We changed experimental plans to try more in vitro experiments. In the end of 2007, animal experiments were restarted and we are now performing knockout mice experiments.
(1) Clinical histopathological study of the ovarian tissues from the cancer patients treated with anticancer drugs preoperatively : We have found several differences between histopathological damages in the ovaries and administered anticancer drugs.
(2) Molecular mechanisms of anticancer drug-induced granulosa cell damages in mouse experiments: (1) We have identified 2 candidate molecules that played an important roles in CPT-11-induced granulosa cell apoptosis. (2) Roles of Bcl-2 family and p53 in several anticancer drag induced ovarian damages were evaluated (these data are preparing in publication). (3) Possible key molecules in granulosa cell damages induced by the other anticancer drugs but CPT-11 were identified The molecules are confirming now. (4) A certain knockout mouse was found to be insensitive to CPT-11-induced granulosa cell apoptosis. The data will be published in 2008.
(3) Establishment of preventive therapy for anticancer drug induced granulosa cell apoptosis : Optimal protocols of GnRHa therapy to prevent anticancer drug-induced granulosa cell apoptosis were established Moreover, the preventive mechanisms were also identified. The data will be published in 2008.
(4) The mouse ovary organ culture was established to assay to evaluate direct ovarian damages by various drugs.
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Report
(3 results)
Research Products
(14 results)
