| Project/Area Number |
18591811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
TAKAI Yasushi (2007-2009) Saitama Medical University, 医学部, 准教授 (60323549)
竹田 省 (2006) 埼玉医科大学, 医学部, 教授 (20143456)
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| Co-Investigator(Kenkyū-buntansha) |
石原 理 埼玉医科大学, 医学部, 教授 (70176212)
高井 泰 埼玉医科大学, 医学部, 講師 (60323549)
|
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIHARA Osamu 埼玉医科大学, 医学部, 教授 (70176212)
TAKEDA Satoru 順天堂大学, 医学部, 教授 (20143456)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,120,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | 卵巣 / 凍結保存法 / 性同一性障害 / アポトーシス |
|---|
| Research Abstract |
Ovarian tissue cryopreservation and transplantation are becoming increasingly important issues for preserving fertility for young women with cancer as shown by recent successes in restoring ovarian activity and even fertility. Vitrification seems to be a promising technology in ovarian tissue cryopreservation, but it is still in an initial stage. Human ovarian tissue was obtained from consenting female-to-male GID (gender indentiry disorder) patients in sex reassignment surgery (SRS). Ovarian cortex tissues were cut into slices with the size of 1×10×10mm, which is suitable for transplantation in clinical situation, and cryopreserved by Cryotissue vitrification protocol. The frozen/thawed human ovarian tissues were xenografted into the ovarian bursa of non-obese diabetic severe combined immunodeficient (NOD-SCID) mice, and at least 1000 viable follicles were observed in each slice two weeks after transplantation.
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