Development of human ovarian tissue cryopreservation technique using ovaries derived from gender identity disorder patients
Project/Area Number |
18591811
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Obstetrics and gynecology
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Research Institution | Saitama Medical University |
Principal Investigator |
TAKAI Yasushi (2007-2009) Saitama Medical University, 医学部, 准教授 (60323549)
竹田 省 (2006) 埼玉医科大学, 医学部, 教授 (20143456)
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Co-Investigator(Kenkyū-buntansha) |
石原 理 埼玉医科大学, 医学部, 教授 (70176212)
高井 泰 埼玉医科大学, 医学部, 講師 (60323549)
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Co-Investigator(Renkei-kenkyūsha) |
ISHIHARA Osamu 埼玉医科大学, 医学部, 教授 (70176212)
TAKEDA Satoru 順天堂大学, 医学部, 教授 (20143456)
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Project Period (FY) |
2006 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥4,120,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
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Keywords | 卵巣 / 凍結保存法 / 性同一性障害 / アポトーシス |
Research Abstract |
Ovarian tissue cryopreservation and transplantation are becoming increasingly important issues for preserving fertility for young women with cancer as shown by recent successes in restoring ovarian activity and even fertility. Vitrification seems to be a promising technology in ovarian tissue cryopreservation, but it is still in an initial stage. Human ovarian tissue was obtained from consenting female-to-male GID (gender indentiry disorder) patients in sex reassignment surgery (SRS). Ovarian cortex tissues were cut into slices with the size of 1×10×10mm, which is suitable for transplantation in clinical situation, and cryopreserved by Cryotissue vitrification protocol. The frozen/thawed human ovarian tissues were xenografted into the ovarian bursa of non-obese diabetic severe combined immunodeficient (NOD-SCID) mice, and at least 1000 viable follicles were observed in each slice two weeks after transplantation.
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Report
(6 results)
Research Products
(48 results)
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[Journal Article] Caspase-12 compensates for lack of caspase-2 and caspase-3 in female germ cells2007
Author(s)
Takai Y, Matikainen T, Jurisicova A, Kim MR, Trbovich AM, Fujita E, Nakagawa T, Lemmers B, Flavell RA, Hakem R, Momoi T, Yuan J, Tilly JL, Perez GI
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Journal Title
Apoptosis 12
Pages: 791-800
Related Report
Peer Reviewed
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[Journal Article] Absence of the proapoptotic Bax protein extends fertility and alleviates age-related health complications in female mice2007
Author(s)
Perez GI, Jurisicova A, Wise L, Lipina T, Kanisek M, Bechard A, Takai Y, Hunt P, Roder J, Grynpas M, Tilly JL
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Journal Title
Proc Natl Acad Sci U S A 104
Pages: 5229-5234
Related Report
Peer Reviewed
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[Journal Article] In vitro formation of capillary networks using optical lithographic techniques2007
Author(s)
Kobayashi A, Miyake H, Hattori H, Kuwana R, Hiruma Y, Nakahama K, Ichinose S, Ota M, Nakamura M, Takeda S, Morita I
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Journal Title
Biochem Biophys Res Commun 358
Pages: 692-697
Related Report
Peer Reviewed
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[Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植.2009
Author(s)
高井泰, 新海愛子, 梅沢優子, 関博之, 鈴木宏志, Aidiresi A, 竹田省, 香川則子, 桑山正成, 井上太綬, 石原理
Organizer
第27回日本受精着床学会総会・学術講演会
Place of Presentation
京都
Related Report
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