| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Purpose
This research is designed for risk evaluation of human papillomavirus (HPV) types and variants for cervical carcinogenesis. In this study, HPV sequences of cervical cells of cervical cancer, dysplasia, and normal control groups were determined, and evaluated risks of detected HPVs.
Material and Methods
HPV sequences of cells of cervical carcinoma group, dysplasia group, and cytologically normal group (conrtol goup) were determined. The L1 region of HPV sequences of cells were amplified with consensus primer set, and subsequently, direct sequencing was performed using sequencing primers. Risk evaluation of each HPV types and variants was determined by odds ratios of the conrol group between the carcinoma group.
Results
The number of dentified HPV types was thirty-five, ranging from type 6 to type 91, including unclassified four types. Among them, type 33 and type 58 were the high-risk types in the elderly population.
Total of 85 genotypes of HPV were identified in this study. HPV types 16, 18, 31, 33, 35, 52, 58, world-wide popular types, have several variants. Among them, three out of five variants of type 16, all four variants of type 18, one out of two variants of type 31, one out of two variants of type 33, one out of three variants of type 35, three out of five variants of type 52, all two variants of type 58 were detected in the invasive carcinoma. Although classified into the high-risk HPV, at least one variants of types 45, 51, 53, 56, 59, 66, 68, 73 were not detected in the invasive carcinoma. Reversely, classified into the low-riks HPV, at least one variants of types 54, 90, 91 were detected in the invasive carcinoma.
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