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Identification and clinical application of novel antiapoptotic factor produced from ovarian cancer cells

Research Project

Project/Area Number 18591843
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionWakayama Medical University

Principal Investigator

TANAKA Tetsuji (2007)  Wakayama Medical University, 医学部, Associated Professor (80275255)

粉川 克司 (2006)  和歌山県立医科大学, 医学部 (80254548)

Co-Investigator(Kenkyū-buntansha) KOKAWA Katsuji  Wakayama Medical University, 医学部, Assistant Professor (80254548)
UMESAKI Naohiko  Wakayama Medical University, 医学部, Professor (20106339)
UTSUNOMIYA Hirotoshi  Wakayama Medical University, 医学部, Assistant Professor (60264876)
YAGI Shigetaka  Wakayama Medical University, 医学部, Assistant (60372869)
田中 哲二  和歌山県立医科大学, 医学部, 助教授 (80275255)
田中 和東  和歌山県立医科大学, 医学部, 助手 (00372863)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsapoptosis / ovarian cancer / drug resistance
Research Abstract

The animal center of our university had been contaminated with mouse hepatitis virus from 2006 to 2007. All the experimental animals including our mice were killed at first, and then the center was disinfected. However, 6 months after the disinfection, virus was found out again in the animal center. We changed experimental plans to try more in vitro experiments. In the end of 2007, animal experiments were restarted and we are now performing mouse hybridoma experiments. In 2007, the first head investigator was retired. Therefore, unexpectedly the first experimental plans could not performed completely and now we have been continuing the original plans.
(1) Biochemical characterization of M-cell derived antiapoptotic substances (MAAS): We partially purified MAAS from the culture supernatants and immunized Bal b/c mice to mace specific antibody-producing hybridoma. However, all the immunized mice were killed because of the contamination accident in the animal center. Since reopening of the animal center, animal experiments have been restarted. Establishment of the monoclonal antibody will make possible to isolate pure MAAS molecules and clone their cDNA.
(2) Biological characterization of anti-apoptotic phenomena by MAAS: (1) We have been investigating molecular mechanisms of anticancer drug-induced ovarian failure in the other experiments. By chance, those experiments had found possible antiapoptotic mechanisms similar to MAAS-induced antiapoptosis. (2) We have established several CDDP-resistant cell lines from M cells. Two CDDP-resistant cell lines produced much higher MAAS activity than that the parent cells. These data indicate that increased MAAS activity can be a possible mechanism of an acquired CDDP resistance.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 6 results) Presentation (2 results)

  • [Journal Article] Experimental cnaractenzation of recurrent ovarian immature teratoma cells after optimal surgery2008

    • Author(s)
      Tanaka T, Toujirm S, et. al.
    • Journal Title

      Oncology Reports (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Experimental characterization of, recurrent ovarian immature teratoma cells after optimal surgery2008

    • Author(s)
      Tanaka T, Toujima S, et. al.
    • Journal Title

      Oncology Reports (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery.2008

    • Author(s)
      Tanaka T, Toujima S, et. al.
    • Journal Title

      Oncology Reports (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous carcinoma cells in vitro2007

    • Author(s)
      Tanaka T, Yukawa K, et. al.
    • Journal Title

      European Journal of Gynaecological Oncology 28

      Pages: 352-355

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Irradiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro2007

    • Author(s)
      Tanaka T, Yukawa K, et. al.
    • Journal Title

      European Journal of Gynaecological Oncology 28

      Pages: 273-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous carcinoma cells in vitro.2007

    • Author(s)
      Tanaka T, Yukawa K, et. al.
    • Journal Title

      European Journal of Gynaecological Oncology 28

      Pages: 352-355

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Irradiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro,2007

    • Author(s)
      Tanaka T, Yukawa K, et. al.
    • Journal Title

      European Journal of Gynaecological Oncology 28

      Pages: 273-277

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous carcinoma cells in vitro.2007

    • Author(s)
      Tanaka T, Yukawa K, et al.
    • Journal Title

      European Journal of Gynaecological Oncology 28(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Irradiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro,2007

    • Author(s)
      Tanaka T, Yukawa K, et al.
    • Journal Title

      European Journal of Gynaecological Oncology 28(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A novel mechanism for acquired cisplatin-resistance : Suppressed translation of death-associated protein kinase mRNA is insensitive to 5-aza-2'-deoxycitidine and trichostatin in cisplatin-resistant cervical squamous cancer cells.2006

    • Author(s)
      Bai T, Tanaka T, et al.
    • Journal Title

      International Journal of Oncology 28

      Pages: 497-508

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro.2006

    • Author(s)
      Tanaka T, Bai T, et al.
    • Journal Title

      Oncology Reports 15(4)

      Pages: 939-947

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effective chemoradiotherapeutic protocol with 5-fluorouracil for cervical squamous cell carcinoma in vitro.2006

    • Author(s)
      Tanaka T, Yukawa K, et al.
    • Journal Title

      European Journal of Gynaecological Oncology 27(3)

      Pages: 243-246

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Apoptpsis in crevical cancer after balloon-occluded arterial infusion of anticancer drugs.2006

    • Author(s)
      Kokawa K, Mabuchi Y, et al.
    • Journal Title

      Anticancer Research 26

      Pages: 1413-1418

    • Related Report
      2006 Annual Research Report
  • [Presentation] 根治手術後の再発性卵巣未熟奇形種を想定した腫瘍性格解析2007

    • Author(s)
      田中 哲二, ほか
    • Organizer
      第59回日本産科婦人科学会学術講演会
    • Place of Presentation
      国立京都国際会館
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery2007

    • Author(s)
      Tanaka T, Toujima S, et. al.
    • Organizer
      The 59th Japanese Comference of Obstetrics & Gynecology
    • Place of Presentation
      National Kyoto Hall
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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