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Non-invasive treatment of uterine fibroid by feeding vessel occlusion using HIFU sonication.

Research Project

Project/Area Number 18591846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShowa University

Principal Investigator

ICHIZUKA Kiyotake  Showa University, School of Medichine, assistant (00338451)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Thetsuya  Showa University, School of Medichine, assistant (60338449)
ICHIHARA Mitsuyushi  Showa University, School of Medchine, assistant (80424274)
UMEMURA Shinichirou  Kyoto University, School of Medicine, professor (20402787)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsHIFU / Uterine fibroid / Feeding artery occlusion / 栄養血管閉塞 / 動物実験モデル
Research Abstract

The final goal of our study is to develop the non-invasive therapy for uterine fibroid by feeding vessels occlusion using high intensity focused ultrasound (HIFU). As a step for the goal, following basic studies have been conducted.1) Measurement of blood flow to uterine fibroid; A micro bubble solution, Levovist^R, was administrated to the patient of uterine fibroid. Increases in the intensity of signal from the tissue were measured by pulsing interval times (0.5, 1, 3, 5 seconds) and blood flow indices were calculated. The indices of tissue blood flow of uterine fibroids were compared with those of the myometrium. The result showed lower tissue blood flow to uterine fibroid than myometrium.2) Animal experiments for artery occlusion; HIFU was sonicated to deep femoral arteries of Sprague Dawley rats. Duration of sonication was 5seconds. Blood flow occlusion was accomplished by HIFU sonication at 4300 W/cm^2, but the flow was preserved with intensities at 530, 1080 and 2750 W/cm^2. Pea … More k systolic velocity of blood flow increased in sonicated arteries with HIFU at 1080 and 2750 W/cm^2 and correlated with HIFU intensity. Histological studies have demonstrated vacuolar degeneration in tunica media of the vessels exposed to HIFU at 2750 and 4300 W/cm^2. Injecting ultrasound contrast agents (Levovist^R) decreased the HIFU intensity required for arterial occlusion, because microbubbles were destroyed under ultrasound sonication and exerted a cavitation effect on the vessel endothelium, which would damage blood vessels.3)Reducing effect of tumor by occlusion of the tumor feeding artery, VX2 (dermatological squamous cancer cell) was implanted in the right femoral muscles of Japanese White rabbits. After 3 weeks, animals were provided to experiment of HIFU sonication to feeding arteries (Peak intensity; 4kW/cm^2). In the control group, the tumor volume increased significantly in comparison with before HIFU sonication. On the other hand, in the study group, the tumor volume showed a tendency to increase, but the significance was not recognized. HE staining revealed vacuolar degeneration in the tunica media of the artery and degenerative necrosis in the tumor.4) Occlusion of the human uterine fibroid feeding artery; A total of 6 patients with uterine fibroids, who had been scheduled for hysterectomy were enrolled in this study. HIFU was applied after laparotomy. Peak intensities were 2 (n=4) and 4 (n=5) kW/cm^2. In those fibroid arteries to which HIFU had been applied at 2 kW/cm^2, the HE stain revealed a vacuolar degeneration in the tunica media of the artery, although cessation of blood flow was not accomplished. The uterine fibroids artery could be successfully occluded by HIFU at 4 kW/cm^2 in two cases out of five. In these cases, the HE stain revealed strong vacuolar degeneration in the tunica media and collapsed cavity. It has been demonstrated by our study that HIFU sonication can achieve the occlusion of deep arteries. Additionally to this fact, low tissue blood flow to uterine fibroid also support that, occlusion of tumors' feeding by HIFU may be an option for non-invasive therapy for uterine fibroid. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2007

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model2007

    • Author(s)
      K.Ichizuka, S.Ando, et. al.
    • Journal Title

      Ultrasound Obstet Gynecol. 22(5)

      Pages: 358-360

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 子宮筋腫の発育制御とその管理 強出力集束超音波(HIFU)を用いた栄養血管閉塞による子宮筋腫の低侵襲治療2007

    • Author(s)
      市塚 清健
    • Journal Title

      日本産科婦人科学会雑誌 59(9)

      Pages: 1703-1711

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model2007

    • Author(s)
      K., Ichizuka, S., Ando, et. al.
    • Journal Title

      Ultrasound Obstet Gynecol Vol. 22(5)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model2007

    • Author(s)
      K. Ichizuka, S. Ando, et. al.
    • Journal Title

      Ultrasound Obstet Gynecol. 25(5)

      Pages: 358-360

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 子宮筋腫の発育制御とその管理強出力集束超音波(HIFU)を用いた栄養血管閉塞による子宮筋腫の低侵襲治療2007

    • Author(s)
      市塚 清健
    • Journal Title

      日本産科婦人科学会雑誌 59(9)

      Pages: 1703-1711

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Blood flow occlusion via ultrasound image-guided high-intensity focused ultrasound and its effect on tissue perfusion.2007

    • Author(s)
      Ichihara M
    • Journal Title

      Ultrasound Med Biol. Jan 5; [Epub shead of print]

    • Related Report
      2006 Annual Research Report
  • [Presentation] 子宮筋腫の発育制御とその管理 強出力集束超音波(HIFU)を用いた栄養血管閉塞による子宮筋腫の低侵襲治療2007

    • Author(s)
      市塚 清健
    • Organizer
      第59回日本産科婦人科学会学術講演会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Non-invasive treatment of uterine fibroid by feeding vessel occlusion using HIFU sonication2007

    • Author(s)
      Kiyotake, Ichizuka
    • Organizer
      59th annual congress of the Japan society of Obstetrics and Gynecology
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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