Identification of genes associated with genesis of epithelial ovarian Identification of genes associated with genesis of epithelial ovarian cancer using non-tumorigenic immortalized ovarian epithelial cell lines

• Project/Area Number: 18591848
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: TAKANO Hirokuni Jikei University School of Medicine, 医学部, 講師 (50226809)
• Co-Investigator(Kenkyū-buntansha): YAMADA Kyosuke 東京慈恵会医科大学, 医学部, 講師 (30230452) ; TAKAKURA Satoshi 東京慈恵会医科大学, 医学部, 講師 (60256401) ; OKAMOTO Aikou 東京慈恵会医科大学, 医学部, 准教授 (20204026) ; OCHIAI Kazunori 東京慈恵会医科大学, 医学部, 教授 (20152514)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,060,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 卵巣癌 / 封入嚢腫 / MET / ゲノム発現解析 / 不死化細胞 / マイクロアレイ / 正常卵巣上皮不死化細胞 / 正常卵巣上皮不死化細胞株

Research Abstract

卵巣癌発生に関与する遺伝子の検索のため、正常卵巣上皮不死化細胞株(IOSEC)を6株樹立した。正常卵巣上皮(OSE)、封入嚢腫(IC)、樹立したIOSECを用いて,種々の抗原マーカーの発現を免疫組織学的に検討し、ICはMenchymal to Epithelial Transition(MET)過程にあることが示唆された。さらにIOSECとそのprimary culture(PC)細胞間で発現が異なる遺伝子を包括的ヒトゲノム発現解析によりスクリーニングし、卵巣癌発生に関する遺伝子を104種類に絞り込んだ。

Research Products

• [Journal Article] Mesenchymal to epithelial transition in the human ovarian surface epithelium focusing on inclusion cysts (2009)
  • Author(s): Okamoto S, Okamoto A, Nikaido T, Saito M, Takao M, Yanaihara N, Takakura S, Ochiai K, Tanaka T
  • Journal Title: Oncol Rep 21 Pages: 1209-1214
  • Peer Reviewed
• [Journal Article] Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation (2009)
  • Author(s): Omi H, Okamoto A, Nikaido A, Urashima M, Kawaguchi R, Umehara N, Sugiura K, Tanaka T
  • Journal Title: Int Mol Med 23 Pages: 229-36
  • Peer Reviewed
• [Journal Article] Mesenchymal to epithelial transition in the human ovarian surface epithelium focusing on inclusion cysts (2009)
  • Author(s): Okamoto S, Okamo to A, Nikaido T, Saito M, Takao M, Yanaihara N, Takakura S, Ochiai K, Tanaka T
  • Journal Title: Oncol Rep 21 Pages: 1209-1214
  • Peer Reviewed
• [Journal Article] Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation (2009)
  • Author(s): Okamoto A, Nikaido T, Urashima M, Kawaguchi R, Umehara N, Sugiura K, Saito M, Kiyono T, Tanaka T
  • Journal Title: Int J Mol Med. 23 Pages: 229-36
  • Peer Reviewed
• [Journal Article] Increased synthesis of indoleamine-2, 3-dioxygenase protein is positively associated with impaired survival in patients with serous-type, but not with other types of, ovarian cancer (2007)
  • Author(s): Takao M, Okamoto A, Nikaido T, Urashima M, Takakura S, Saito M, Saito M, Okamoto S, Takikawa O, Sasaki H, Yasuda M, Ochiai K, Tanaka T
  • Journal Title: Oncol Rep 17 Pages: 1333-9
  • Peer Reviewed
• [Journal Article] Association of extracellular matrix metalloproteinase inducer in endometrial carcinoma with patient outcomes and clinicopat hologenesis using monoclonal antibody 12C3. (2007)
  • Author(s): Ueda K, Yamada K, Urashima M, Ishibashi Y, Shirai M, Nikaido T, Takahashi H, Okamoto A, Saito M, Yasuda M, Qhkawa K, Tanaka T.
  • Journal Title: Oncol Rep. 17 Pages: 731-5
  • Peer Reviewed
• [Journal Article] Increased synthesis of indoleamine-2,3-dioxygenase protein is positively associated with impaired survival in patients with serous-type, but not with other types of, ovarian cancer. (2007)
  • Author(s): Takao M, Okamoto A, Nikaido T, Urashima M, Takakura S, Saito M, Saito M, Okamoto S, Takikawa O, Sasaki H, Yasuda M, Ochiai K, Tanaka T.
  • Journal Title: Oncol Rep. 17 Pages: 1333-9
  • Peer Reviewed
• [Journal Article] IGFBP1 and Follistatin-like 3 genes are significantly up-regulated in expression profiles of the IUGR (2006)
  • Author(s): Okamoto A, Endo H, Kalionis B, Shinya M, Saito M, Nikaido T, Tanaka T
  • Journal Title: Placenta. 27 Pages: 317-2
• [Presentation] Mapping 25K Array-GISTIC解析法を用いた卵巣癌におけるコピー数異常領域の検討 (2008)
  • Author(s): 岡本愛光, 矢内原臨, 高倉聡, 高尾美穂, 尾見裕子、柳田聡、田部宏、山田恭輔, 新美茂樹、佐々木寛、安田允, 落合和徳, 田中忠夫
  • Organizer: 第7回日本婦人科がん分子標的研究会学術集会
  • Place of Presentation: 名古屋
• [Presentation] 第アレイ解析臨床応用を目指して漿液性卵巣癌における包括的ヒトゲノム発現解析によるPaclitaxel(PTX)耐性関連遺伝子のスクリーニングとその臨床応用 (2008)
  • Author(s): 岡本愛光, 浦島充佳, 二階堂孝, 高尾美穂, 斉藤美里, 高倉聡, 矢内原臨, 山田恭輔, 高野浩邦、磯西成治, 安田允, 落合和徳, 田中忠夫
  • Organizer: 18回日本サイトメトリー学会学術集会
  • Place of Presentation: 東京
• [Presentation] アレイ解析 臨床応用を目指して漿液性卵巣癌における包括的ヒトゲノム発現解析によるPaclitaxel(PTX)耐性関連遺伝子のスクリーニングとその臨床応用 (2008)
  • Author(s): 岡本愛光, 浦島充佳, 二階堂孝, 高尾美穂, 斉藤美里, 高倉聡, 矢内原臨, 山田恭輔, 磯西成治, 安田允, 落合和徳, 田中忠夫
  • Organizer: 第18回日本サイトメトリー学会
  • Place of Presentation: 東京
• [Presentation] Molecular approach for individualized therapy in ovarian cancer. (2007)
  • Author(s): Aikou Okamoto, Nikaido T, Urashima M, Takao M, Omi H, Okamoto S, Yanaihara N, Takakura S, Ochiai K, Tanaka T.
  • Organizer: International College of Surgeons, XVIIth Joint Congress of Asia & Pacific Federations & 53rd Annual Congress of the Japan Section
  • Place of Presentation: Kyoto
  • Year and Date: 2007-06-10
• [Presentation] Establishment of an immortalized human extravious trophoblast cell line by retroviral infection of HPV E6/E7 and hTERT. International College of Surgeons, (2007)
  • Author(s): Omi H, Okamoto A, Nikaido T, Saito M, Umehara N, Kawaguchi R, Ikeya M, Sugiura K, Kiyono T, Tanaka T
  • Organizer: XVIIth Joint Congress of Asia & Pacific Federations & 53rd Annual Congress of the Japan Section
  • Place of Presentation: Kyoto
• [Presentation] Examination of IDO Expression in ovarian cancer. International College of Surgeons (2007)
  • Author(s): Takao M, Okamoto A, Nikaido T, Urashima M, Takakura S, Misato S, Okamoto S, Yanaihara N, , Ochiai K, Tanaka T
  • Organizer: XVIIth Joint Congress of Asia & Pacific Federations & 53rd Annual Congress of the Japan Section
  • Place of Presentation: Kyoto
• [Presentation] Molecular approach for individualized therapy in ovarian cancer. International College of Surgeons (2007)
  • Author(s): Okamoto A, Nikaido T, Urashima M, Takao M, Omi H, Okamoto S, Yanaihara N, Takakura S, Ochiai K, Tanaka T
  • Organizer: XVIIth Joint Congress of Asia & Pacific Federations & 53rd Annual Congress of the Japan Section (Invited)
  • Place of Presentation: Kyoto