| Project/Area Number |
18591864
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
TAKEUCHI Kazuhiko Mie University, Graduate school of Medicine, Associate Professor (50206942)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YUTA Atsushi Mie University Hospital, 医学部附属病院, Assistant Professor (80293778)
MAJIMA Yuichi Mie University, Graduate school of Medicine, Professor (60024791)
ISHINAGA Hajime Mie University, Graduate achool of Medicine, Assistant (50335121)
SAKAIDA Hiroshi Mie University Hospital, 医学部附属病院, Resident (30378426)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,680,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | extracellular matrix / mucin / leukotriene / steroid / nasal mucosa / remodeling / 鼻アレルギー / モルモット / 杯細胞 / 抗原刺激 / 上皮細胞 / 卵白アルブミン |
|---|
| Research Abstract |
The mechanisms by which nasal mucosa remodeling occurs in allergic rhinitis have remained unclear. The aim of this study was to establish a guinea pig model of allergic rhinitis with remodeling and to examine the effects of dexamethasone and pranlukast on nasal mucosa remodeling. Three groups of ovalbumin-sensitized Hartley guinea pigs received daily intranasal challenges with ovalbumin for 1, 8, 12 weeks, respectivelylb examine the effect of dexamethasone and pranlukast, the animals were divided into 4 groups : negative control group ; ovalbumin-sensitized group ; ovalbumin+dexamethasone gmup ; and ovalbumin+pranlukast group. During 12 weeks of intranasal exposure to ovalbumin, the latter two groups received daily intraperitoneal injections of dexamethasone and pranlukast, respectively. Prolonged ovalbumin challenge caused significant pathological changes in the nasal mucosa and conchae, including eosinophil infiltration, significant goblet cell hyperplasia, epithelial damage and deposition of extracellular matrix. In contrast to the negative control group, the ovalbumin-sensitized group exhibited significant goblet cell hyperplasia, epithelial damage and deposition of extracellular matrix. These changes were significantly inhibited by dexamethasone and pranlukast, respectively. We have established a model of upper airway remodeling in guinea pigs. The tissue remodeling was inhibited by early intervention with the and allergic-inflammatory agents dexamethasone and pranlukast
|
