Expression and characterization of B7-H1 in head and neck cancer and development of novel anti-cancer treatment using siRNAagainst B7-H1
| Project/Area Number |
18591867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Okayama University |
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Principal Investigator |
TOMINAGA Susumu Okayama University, Hospital of Medical and Dentistry, Lecturer (70420487)
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| Co-Investigator(Kenkyū-buntansha) |
OKANO Mitsuhiro Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor (60304359)
FUKUSHIMA Kunihiro Okayama University, Hospital of Medical and Dentistry, Lecturer (50284112)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | co-inhibitory molecules / B7-H1 / head and neck cancer / siRNA / recurrence / 免疫寛容 / 免疫染色 / リアルタイムPCR |
|---|
| Research Abstract |
Co-inhibitory molecules are known to induce immune tolerance. Among these, B7-H1 (PD-L1) is expressed in cancer cells, and may cancel anti-tumor immunity by inducing immune tolerance. However, little is known whether B7-H1 is expressed in head and neck cancers in Japanese population
We investigated the expression and characterization of B7-H1 in head and neck cancers in Japanese population. Immunohistochemical staining revealed that B7-H1 protein is expressed in tongue, oral, pharyngeal and laryngeal cancers. RT-PCR analysis confirmed the expression of this molecule in head and neck cancers at mRNA level. Next, we determined the amounts of B7-Hl mRNA in head and neck cancers by real-time PCR, and analyzed the correlation of the amounts of B7-H1 mRNA and clinical characteristics. No significant correlation was seen between the amounts of B7-H1 mRNA and staging including tumor size, lymph node metastasis, distinct metastasis, recurrence, or survival rate.
In this project, we tried knock-down of B7-H1 in SCCVII cell, the mouse squamous cell carcinoma cell line, using siRNA. Flow cytometric analysis revealed that SCCVII cell expressed B7-Hl. Template DNA for whole transcription of RNA for B7-H1 was made, and dsRNA was generated. Then siRNA was made by fragmentation of dsRNA by Dicer siRNA was transfected into SCCVII. However, decrease in B7-H1 expression in SCCVII was marginal (about 10%). The change of B7-H1 expression was similar when we changes the buffets for transfection from GeneSilencer to siIMPORTER, lipofectamine, and RNAiMAX.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] CRTH2 plays an essential role in the pathophysiology of Cry j 1-induced pollinosis in mice2008
Author(s)
Nomiya, R., Okano, M., Fujiwara, T., Maeda, M., Kimura, Y., Kino, K., Yokoyama, M., Hirai, H., Nagata, K., Hara, T., Nishizaki, K., Nakamura, M
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Journal Title
Journal of Immunology 180(8)
Pages: 5680-5688
NAID
Description
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Author(s)
Sugata, Y., Okano, M., Fujiwara, T., Matsumoto, R., Hattori, H., Yamamoto, M., Nishibori, M., Nishizaki, K
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Journal Title
Immunology 121(2)
Pages: 266-275
Description
「研究成果報告書概要(欧文)」より
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