Arole of cell membrane microdomains in air-liquid interface-induced invasive growth of laryngeal carcinoma cells

• Project/Area Number: 18591871
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Saga University
• Principal Investigator: TODA Shuji Saga University, Pathology & Biodefense, Professor (80188755)
• Co-Investigator(Kenkyū-buntansha): KURATOMI Yuichiro Saga University, Otolaryngology, Associate Professor (30225247)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Air-liquid interface / Laryngeal cancer / Sqaumous cell carcinoma / Cell membrane microdomains / Caveolin / Growth / Invasion / Cholesterol inhibitor / 咽頭癌

Research Abstract

Air-liquid interface (ALI) promotes the invasive growth of squamous cell carcinoma (SCC) cells of the larynx in vitro. ALI-induced invasive growth of SCC cells is related to the following events. 1. The cell membrane microdomain caveolin is considered to be activated in SCC cells by ALI stimulation. 2. In ALI-induced invasive growth of SCC cells, caveolin, Ras/MAPK pathway molecules, MMP and filamin Aare enhanced, but it is unclear whether caveolin activation is associated with the enhancement of Ras/MAPK pathway molecules, MMP and filamin A 3. Cholesterol inhibitor is considered to prohibit ALI-induced invasive growth of SCC cells through caveolin inactivation that may induce the downregulation of Ras/MAPK pathway molecules, MMP and filamin A 4. ALI promotes the invasive growth of SCC cells in their planting nude mice, but ALI does not affect the metastasis of SCC cells. SCC cells in vivo express the cell membrane microdomain caveolin, but the inhibition of caveolin expression does not affect the invasive growth of SCC cells, suggesting that caveolin may be a responsible factor for the invasive growth of SCC cells in vivo. 5. Cholesterol inhibitor does not affect the growth, invasion and metastasis of SCC cells in their planting nude mice.

Research Products

• [Journal Article] Hypopharyngeal squamous cell carcinoma bordering ectopic gastric mucosa "inlet patch" of the cervical esophagus (2007)
  • Author(s): Satoh S, Nakashima T, Watanabe K, Toda S, Kuratomi Y, Sugihara H, Inokuchi A
  • Journal Title: Auris Nasus Larynx 34 Pages: 135-139
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hypopharyngeal squamous cell carcinoma bordering ectopic gastric mucosa "inlet patch" of the cervical esophagus. (2007)
  • Author(s): Satoh, S., Nakashima, T., Watanabe, K., Toda, S., Kuratomi, Y., Sugihara, H., Inokuchi, A
  • Journal Title: Auris Nasus Larynx. 34 Pages: 135-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 放射線被爆線維芽細胞は癌扁平上皮癌細胞の浸潤性増殖を促進する (2007)
  • Author(s): 蒲地 紀之, 内橋 和芳, 青木 茂久, 戸田 修二, 杉原 甫, 工藤 祥
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Irradiated fibroblasts promote invasive growth of squamous cell carcinoma. (2007)
  • Author(s): Noriyuki, Kamochi, Kazuyoshi, Uchihashi, Shigehisa, Aoki, Shuji, Toda, Hajime, Sugihara, Sho, Kudoh
  • Organizer: The 96 th Annual Meeting of Japanese Society of Pathology
  • Place of Presentation: Osaka
  • Year and Date: 2007-03-15
  • Description: 「研究成果報告書概要(欧文)」より