| Project/Area Number |
18591873
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TAKAHASHI Haruo Nagasaki University, Graduate School of Biomedical Siences, Department of Otolaryngology Head and Neck Surgery, Professor (90171511)
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| Co-Investigator(Kenkyū-buntansha) |
KOUJI Takehiko Nagasaki University, Graduate School of Biomedical Sciences, Department of Otolaryngology Head and Neck Surgery, Professor (30170179)
FUKUDA Tomomi Nagasaki University, Hospital of Medicine and Dentistry, staff (40372776)
UMEKI Hiroshi Nagasaki University, Hospital of Medicine and Dentistry, staff (10404310)
FUJIYAMA Daisuke Nagasaki University, Hospital of Medicine and Dentistry, staff (70437870)
宗 英吾 長崎大学, 医学部歯学部附属病院, 助手 (30404221)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | apoptosis / mastoid / osteoblast / osteoclast / otitis media / TUNEL staining / pneumatization |
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| Research Abstract |
To examine the presence or absence of apoptotic participation in the process of normal development of the mastoid pneumatization in human, we performed immunohistochemical examinations of the tissue cells in the mastoid pneumatized bones harvested from 26 patients without history or signs of otitis media, who underwent mastoid surgery in our department clinic. We used the TdT-mediated dUTP nick end-labeling (TUNEL)method for observing apoptosis cells, which revealed TUNEL positive cells in 13 of 26 cases, and these cells were found in 84.6% of children under the age of 5,being significantly greater number than in those older than 6 years of age. Furthermore, in a sample from a child with otitis media, TUNEL positive cell was not observed. These results can be interpreted as having a good correlation between the presence or absence of TUNEL positive cells and growth of mastoid pneumatization, and indicated that apoptosis might contribute to the normal growth of mastoid pneumatization. By identifying those TUNEL positive cells and by examining cytokines related to those cells, the cell biological mechanisms of growth of pneumatization and thus the mechanism and protective measures of its inhibition.
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