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Analysis of apoptotic pathway in olfactory receptor neurons which causes olfactory disturbance and inhibition of apoptotic cell death

Research Project

Project/Area Number 18591901
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionThe University of Tokyo (2007-2008)
独立行政法人国立病院機構(東京医療センター臨床研究センター) (2006)

Principal Investigator

SAKAMOTO Takashi  The University of Tokyo, 医学部・附属病院, 助教 (50323548)

Co-Investigator(Kenkyū-buntansha) 角田 晃一  独立行政法人国立病院機構東京医療センター臨床研究センター, 人工臓器機器開発研究部, 部長 (30197751)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords嗅覚 / 嗅細胞 / アポトーシス / 耳鼻咽喉科学
Research Abstract

メチマゾールによる嗅粘膜細胞障害は、免疫染色・TUNEL染色・カスパーゼ活性のアッセイ・ウエスタンブロットによる細胞質分画のチトクロムcの定量の結果からミトコンドリア経路によるアポトーシスであることが示唆された。この障害はカスパーゼ-3・-9阻害剤の投与、強力なBcl-XL活性を有する合成蛋白PTD-FNK投与により抑制された。さらに、障害細胞では8-OHdG、4-HNE、活性化p38MAPKの発現が亢進していたことからメチマゾールによる嗅粘膜細胞のアポトーシスは酸化ストレスの増加→p38MAPK の活性化によって生じていることが示された。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (13 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa : age-related progression2009

    • Author(s)
      Kondo K, Watanabe K, Sakamoto T, Suzukawa K, Nibu K, Kaga K, Yamasoba T
    • Journal Title

      Cell Tissue Res. 335(3)

      Pages: 489-503

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 外傷性外リンパ瘻の治療2008

    • Author(s)
      坂本幸士、山岨達也
    • Journal Title

      ENTONI 94

      Pages: 38-42

    • Related Report
      2008 Annual Research Report
  • [Journal Article] C/EBPβ promotes transition from proliferation to hypertrophic differentiation of chondrocytes through transactivation of p57Kip22008

    • Author(s)
      Makoto Hi rata, et al.
    • Journal Title

      PLoS ONE 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A protein derived from the fusion of TAT peptide and FNK, a BCL-XL derivative, prevents cochlear hair cell death from aminoglycoside ototoxicity in vivo2007

    • Author(s)
      Kashio A, Sakamoto T, Suzukawa K, Asoh S, Ohta S, Yamasoba T
    • Journal Title

      J Neurosci Res 85(7)

      Pages: 1403-12

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methimazole-induced cell death in rat olfactory receptor neurons occurs via apoptosis triggered through mitochondrial cytochrome c-mediated caspase-3 activation pathway2007

    • Author(s)
      Sakamoto T, Kondo K, Kashio A, Suzukawa K, Yamasoba T
    • Journal Title

      J Neurosci Res. 85(3)

      Pages: 548-57

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methimazole-induced cell death in rat olfactory receptor neurons occurs via apoptosis triggered through mitochondrial cytochrome c-mediated caspase-3 activation pathway.2007

    • Author(s)
      Sakamoto T
    • Journal Title

      Journal of Neuroscience Research 85(3)

      Pages: 548-557

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A protein derived from the fusion of TAT peptide and FNK, a BCL-XL derivative, prevents cochlear hair cell death from aminoglycoside ototoxicity in vivo2007

    • Author(s)
      Kashio A
    • Journal Title

      Journal of Neuroscience Research 85(7)

      Pages: 1403-1412

    • Related Report
      2006 Annual Research Report
  • [Presentation] アポトーシス抑制蛋白質PTD-FNKによる薬剤性嗅上皮障害抑制の試み2008

    • Author(s)
      坂本幸士
    • Organizer
      東京大学生命科学研究ネットワークシンポジウム2008
    • Place of Presentation
      東京大学安田講堂
    • Year and Date
      2008-09-23
    • Related Report
      2008 Final Research Report
  • [Presentation] アポトーシス抑制蛋白質PTD-FNKによる薬剤性嗅上皮障害掬制の試み2008

    • Author(s)
      坂本幸士
    • Organizer
      東京大学生命科学研究ネットワークシンポジウム2008
    • Place of Presentation
      東京大学安田講堂
    • Year and Date
      2008-09-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Transcriptional regulation of osteoarthritis progression2008

    • Author(s)
      Hiroshi Kawaguchi
    • Organizer
      The 13th Congress of the Asia Pacific League of Associations for Rheumatology (APLAR 2008).
    • Place of Presentation
      Yokohama
    • Related Report
      2008 Annual Research Report
  • [Presentation] 抗甲状腺剤メチマゾール投与によって誘発されるラット嗅細胞障害の障害様式に関する検討2007

    • Author(s)
      坂本幸士
    • Organizer
      日本鼻科学会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2007-09-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 抗甲状腺剤メチマゾール投与によって誘発されるラット嗅細胞障害の障害様式に関する検計2007

    • Author(s)
      坂本 幸士、山岨 達也
    • Organizer
      第46回日本鼻科学会総会・学術講演会
    • Place of Presentation
      栃木県宇都宮市
    • Year and Date
      2007-09-28
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.h.u-tokyo.ac.ip/ortho/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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