| Project/Area Number |
18591918
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
FUJIKADO Takashi Osaka University, Graduate School of Medicine, Professor (50243233)
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| Co-Investigator(Kenkyū-buntansha) |
KUSAKA Shumji Osaka University, Graduate School of Medicine, Associate Professor (60260387)
MATSUSHITA Kenji Osaka University, Graduate School of Medicine, Assistant Professor (40437405)
MIYOSHI Tomomitsu Osaka University, Graduate School of Medicine, Assistant Professor (70314309)
大鳥 安正 大阪大学, 医学系研究科, 講師 (40303953)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Neuroprotection / Trans-corneal electrical stimulation / Traumatic Optic Neuropathy / Ischemic Optic Neuropathy / IGF-1 / 電気刺激 / 神経栄養因子 / 視神経症 / 緑内障 |
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| Research Abstract |
(1). Molecular mechanism of neroprotection for the retina and optic nerve by trans-corneal electrical stimulation.
Based on our previous report that trans-corneal electrical stimulation to the retina prevented neuronal death of retinal ganglion cells with up-regulation of Insulin-like growth factor 1(IGF1), we studied if IGF-1 was up-regulated by electrical stimulation to the cultured Mueller cells using RT-PCR. The result was that the mRNA of IGF-1 significantly increased if the mRNA was extracted just after the cessation of 30 minutes electrical stimulation. The Ca++ imaging showed that intracellular Ca++ concentration increased just after the start of electrical stimulation, reached 1.5 fold at 30minutes. The increase of Ca++ concentration was completely blocked by the application of Nifedipine, an L-type Ca++ channel blocker. These results indicated that the electrical stimulation promotes the transcription of IGF-1 gene and this phenomenon is related to the increase of intracellular concentration of Ca++ through the L-type Ca++ channel.
(2). Clinical investigation of trans-corneal electrical stimulation to the optic neuropathy
We investigated the effect of trans-corneal electrical stimulation (TcES) to the eye with ischemic optic neumpathy (ION) or traumatic optic neumpathy (TON). Consecutive chromic patients with ION (20 eyes) and TON (10 eyes) who visited Osaka University hospital between June 2003 and May 2006 in which TcES was applied with an interval of 6 to 8 weeks were examined. The increase of visual acuity greater than 0.3 Log MAR unit were observed in 6 eyes (30%) of ION and 5 eyes (50%) of TON. No patient decreased vision more than 0.3 Log MAR unit. These data suggests that TcES is effecive in some patients with ION and TON.
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