Development of a New Rabbit Model of Age-Related Macular Degeneration

• Project/Area Number: 18591929
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: YASUKAWA Tsutomu Nagoya City University, 大学院・医学研究科, 准教授 (00324632)
• Co-Investigator(Kenkyū-buntansha): OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963) ; SAKURAI Eiji 名古屋市立大学, 大学院・医学研究科, 講師 (30305528)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 眼細胞生物学 / 加齢黄斑変性 / リポフスチン / ドルーゼン / 脈絡膜新生血管 / 家兔 / 動物モデル / 眼底自発蛍光 / 網膜色素上皮

Research Abstract

加齢黄斑変性は成人失明の主要原因である。加齢現象を理解することは病態解明や疾患予防につながるものである。最初の加齢変化は網膜色素上皮細胞内へのリポフスチンと呼ばれる難溶性顆粒の蓄積であるが、本研究では、リポフスチンの成分である最終糖化産物の形成反応により人工的に作製したリポフスチン模擬微粒子を家兎の網膜下に移植することにより、リポフスチン蓄積モデルを作製した。本モデルは加齢黄斑変性の病態を再現しており加齢黄斑変性の病態解明に有用であると考えられた。

Research Products

• [Journal Article] Early-onset macular holes following ruptured retinal arterial macroaneurysms (2008)
  • Author(s): Sato R, Yasukawa T, Hirano Y, Ogura Y.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 246 Pages: 1779-1782
  • Peer Reviewed
• [Journal Article] Suppressive effect of short-interfering RNA on hyperglycemia-induced expression of intercellular adhesion molecule-1 on cultured vascular endothelial cells (2008)
  • Author(s): Takase A, Yasukawa T, Kato A, Ogura Y.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 246 Pages: 989-992
  • Peer Reviewed
• [Journal Article] Large blunt scleral rupture without retinal detachment (2008)
  • Author(s): Takayama K, Yasukawa T, Okada M, Sumida A, Watanabe N, Uchida S.
  • Journal Title: Ophthalmic Surg Lasers Imaging 39 Pages: 242-245
  • Peer Reviewed
• [Journal Article] Suppressive effect of short-interfering-RNA on hyperglycemia-induced expressi on of intercellular adhesion molecule-1 on cultured vascular endothelial cells. (2008)
  • Author(s): Takase A
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 246 Pages: 989-992
  • Peer Reviewed
• [Journal Article] Glycoxidized particles mimic lipofuscin accumulation in aging eyes : a new age-related macular degeneration model in rabbits (2007)
  • Author(s): Yasukawa T, Wiedemann P, Hoffmann S, Kacza J, Eichler W, Wang YS, Nishiwaki A, Seeger J, Ogura Y.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 245 Pages: 1475-1485
  • Peer Reviewed
• [Journal Article] Flexible UV light guiding system for intraocular laser microsurgery (2007)
  • Author(s): Schastak S, Yafai Y, Yasukawa T, Wang YS, Hillrichs G, Wiedemann P.
  • Journal Title: Lasers Surg Med 39 Pages: 353-357
  • Peer Reviewed
• [Journal Article] Copper foreign body in the lens without damage of iris and lens capsule (2007)
  • Author(s): Tokoro M, Yasukawa T, Okada M, Ogura Y, Uchida S.
  • Journal Title: Int Ophthalmol 27 Pages: 329-331
  • Peer Reviewed
• [Journal Article] Development of drug-delivery systems to the posterior segments of the eye (2007)
  • Author(s): Yasukawa T, Tabata Y, Kimura H, Kunou N, Ogura Y.
  • Journal Title: Expert Rev Ophthalmol 2 Pages: 197-211
  • Peer Reviewed
• [Journal Article] Glycoxidized particles mimic lipofuscin accumulation in aging eyes: a new age-related macular degeneration model in rabbits. (2007)
  • Author(s): Yasukawa T
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 245 Pages: 1475-1485
  • Peer Reviewed
• [Journal Article] Drug delivery from ocular implants (2006)
  • Author(s): Yasukawa T, Ogura Y, Kimura H, Sakurai E, Tabata Y.
  • Journal Title: Expert Opin Drug Deliv 3 Pages: 261-273
  • Peer Reviewed
• [Journal Article] Characteristics of iris and retinal pigment epithelial cells cultured on collagen type I membranes (2006)
  • Author(s): Thumann G, Hueber A, Dinslage S, Schaefer F, Yasukawa T, Kirchhof B, Yafai Y, Eichler W, Bringmann A, Wiedemann P.
  • Journal Title: Curr Eye Res. 31 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Drug delivery from ocular implants. (2006)
  • Author(s): Yasukawa T
  • Journal Title: Expert Opin Drug Deliv 3・2 Pages: 261-273
• [Presentation] Fundus autofluorescence in a rabbit age-related macular degeneration model induced by simulation of lipofuscin accumulation. (2008)
  • Author(s): Yasukawa T
  • Organizer: 26^<th> meeting of the Club Jules Gonin
  • Place of Presentation: St. Moritz, Switzerland
  • Year and Date: 2008-09-12
• [Presentation] Three-dimensional spheroid culture system redifferentiates retinal pigment epithelial cells independent of number of passages (2008)
  • Author(s): Yasukawa T, Nishiwaki A, Wiedemann P, Takase A, Yafai Y, Eichler W, Bringmann A, Ogura Y.
  • Organizer: 2008 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2008-05-01
• [Presentation] Three-dimensional spheroid culture system redifferentiates retinal pigme nt epithelial cells independent of number of passages. (2008)
  • Author(s): Yasukawa T
  • Organizer: 2008 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2008-05-01
• [Presentation] Fundus autofluorescence in a rabbit age-related macular degeneration model induced by simulation of lipofuscin accumulation (2008)
  • Author(s): Yasukawa T, Wiedemann P, Hirata M, Takase A, Ogura Y.
  • Organizer: 26th meeting of the Club Jules Gonin
  • Place of Presentation: St. Moritz, Switzerland
• [Presentation] Long-term outcome of radiation therapy for exudative age-related macular degeneration in Japanese patients (2008)
  • Author(s): Yasukawa T, Tokoro M, Sakurai E, Yoshida M, Ogura Y.
  • Organizer: Would Ophthalmology Congress (WOC) 2008
  • Place of Presentation: Hong Kong, China
• [Presentation] Fundus autofluorescence in a rabbit model of age-related macular degenration induced by advanced glycation end product microspheres as imitation lipofuscin (2007)
  • Author(s): Hirata M
  • Organizer: 2007 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2007-05-09
• [Presentation] Drusen formation in a three-dimensional culture system of retinal pigment epithelial cells (2007)
  • Author(s): Yasukawa T, Wiedemann P, Takase A, Nishiwaki A, Hirata M, Kacza J, Yafai Y, Eichler W, Seeger J, Ogura Y.
  • Organizer: 2007 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2007-05-07
• [Presentation] Fundus autofluorescence in a rabbit model of age-related macular degeneration induced by subretinal implantation of imitation lipofuscin (2007)
  • Author(s): Yasukawa T, Wiedemann P, Hirata M, Takase A, Kunou N, Kimura E, Eichler W, Ogura Y.
  • Organizer: SOE The 16th congress of the European Society of Ophthalmology
  • Place of Presentation: Wien, Austria
• [Presentation] Fundus autofluorescence in a rabbit model of age-related macular degenration induced by subretinal implantation of imitation lipofuscin (2007)
  • Author(s): Yasukawa T
  • Organizer: 2007 Annual Meeting of the European Society of Ophthalmology
  • Place of Presentation: Vienna, Austria
• [Presentation] In vitro observasion system for basal functions of retinal pigment epithelium (2006)
  • Author(s): Yasukawa T, Wiedemann P, Kacza J, Takase A, Seeger J, Ogura Y.
  • Organizer: 2006 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2006-05-01
• [Presentation] Drusen in aging eyes : a masterpiece of retinal pigment epithelium (2006)
  • Author(s): Yasukawa T, Wiedemann P, Kacza J, Yafai Y, Eichler W, Hoffmann S, Seeger J, Ogura Y.
  • Organizer: 25th meeting of the Club Jules Gonin
  • Place of Presentation: Cape Town, South Africa