The roles of tenascin-C for corneal epithelial stem cell niche

• Project/Area Number: 18591930
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MASUDA Akira Kyoto Prefectural University of Medicine, Ophthalmology, Research Associate (00312348)
• Co-Investigator(Kenkyū-buntansha): KUSAKABE Moriaki Kyoto Prefectural University of Medicine, Institute for animal reproduction, Investigator (60153277)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: tenascin-C / stem cell niche / beta catenin / 発現制御 / 発生・分化

Research Abstract

Immunohistological analysis of ocular stem cell deficiency diseases revealed massive subconjunctival Tenascin-C (TNC) deposition. TNC reporter gene analysis showed TNC gene induction upon wound healing and mechanical stress. The addition of TNC into the fibronectin (FN) coated dishes induced the change of cell shapes. The TNC knockout mouse analysis showed higher level of beta-catenin in the wild type corneal epithelium in the basal membrane side whereas diffuse beta-catenin expression was observed in TNC knockout mouse. The TNC molecule may contribute to the formation of corneal epithelial stem cell homeostasis through controlling beta-catenin signals.

Research Products

• [Journal Article] Genetic polymorphisms in the promoter of the interferon gamma receptor 1 gene are associated with atopic cataracts (2007)
  • Author(s): Matsuda, A, Ebihara, N, Kumagai, N, Fukuda, K, Ebe, K, Hirano, K, Sotozono, C, Tei, M, Hasegawa, K, Shimizu, M, Tamari, M, Namba, K, Ohno, S, Mizuki, N, Ikezawa, Z, Shirakawa, T, Hamuro, J, Kinoshita, S
  • Journal Title: Invest Ophthalmol Vis Sci 48 Pages: 583-589
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic polymorphisms in the promoter of the interferon gamma receptorl gene are associated with atopic cataracts (2007)
  • Author(s): Matsuda A.
  • Journal Title: Investigative Ophthalmology and Visual Science 48・2 Pages: 583-589
• [Journal Article] Association between ADAM33 polymorphisms and adult asthma in the Japanese population. (2006)
  • Author(s): Hirota T.
  • Journal Title: Clinical and Experimental allegy 36,10 Pages: 884-891
• [Journal Article] High-affinity IgE receptor-β chain expression in human mast cells
  • Author(s): Matsuda A.
  • Journal Title: J.Immunological Methods (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] LOXL1 genetic polymorphisms are associated with exfoliation glaucoma in Japanese
  • Author(s): Mori K.
  • Journal Title: Molecular Vision (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] High-affinity IgE recept or -B chain expression in human mast cells
  • Author(s): Matsuda, A, Okayama, Y, Ebihara, N, Yokoi, N, Gao, P, Hamuro, J, Hopkin, JM, Kinoshita, S
  • Journal Title: J Immunol Methods In press
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] LOXL1 genetic polymorphisms are associated with exfoliation glaucoma in Japanese
  • Author(s): Mori, K, Imai, K, Matsuda, A, Ikeda, Y, Naruse, S, Hitora-Takeshita, H, Nakano M, Taniguchi, T, Omi, N, Tashiro, K, Kinoshita S
  • Journal Title: Mol Vis In press
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-affinity IgE receptor-β chain expression in human mast cells
  • Author(s): Matsuda A.
  • Journal Title: J. Immunological Methods (in press)
  • Peer Reviewed
• [Journal Article] LOXL1 genetic polymorphisms are associated with exfoliation glaucoma in Japanese.
  • Author(s): Mori K.
  • Journal Title: Molecular Vision (in press)
  • Peer Reviewed
• [Presentation] The effects of tenascin-C for beta-catenin signals in co meal epithelial cells (2007)
  • Author(s): Akira, Matsuda, Moriaki, Kusakabe, Satoshi, Kawasaki, Eiichi, Sekiyama, Junji, Hamuro, Shigeru, Kinoshita
  • Organizer: Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO) Fort Lauderdale
  • Place of Presentation: Florida USA
  • Year and Date: 2007-05-09
  • Description: 「研究成果報告書概要(欧文)」より