Analysis of useful molecules in interphotoreceptor matrix for retinal detachment treatment

• Project/Area Number: 18591940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Aichi Medical University
• Principal Investigator: ZAKO Masahiro Aichi Medical University, Ophthalmology, Professor (80298596)
• Co-Investigator(Kenkyū-buntansha): YONEDA Masahiro Aichi Prefectural College of Nursing and Health, Biochemistry, Professor (80201086) ; IWAKI Masayoshi Aichi Medical University, Ophthalmology, Professor (10093163)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: SPACR / SPACRCAN / hyaluronan / interphotoreceptor matrix / FiVerHy versican / FiVerHy / バーシカン / 網膜接着 / 網膜分化 / versican / プロテオグリカン

Research Abstract

We previously showed that chicken SPACR expression increased with developmental age in interphotoreceptor matrix, paralleling the adhesion between neural retina and retinal pigment epithelium (Zako, et al., 2002). As we hypothesized that an appropriate usage of the molecules in interphotoreceptor matrix may assist physiological recovery from retinal detachment, we tried to analyze other molecules there. Here we newly characterized chicken SPACRCAN in interphotoreceptor matrix. Furthermore, we fortunately clarified FiVerHy complex in ciliary body as a result of investigations for the origin of ocular hyaluronan. We also found the degradation of versican, a key molecule for FiVerHy, in chicken vitreous body and in human dermal samples from solar elastosis. In conclusion, we need more information about molecules in interphotoreceptor matrix to utilize these molecules for the treatment of retinal detachment.

Research Products

• [Journal Article] Versican and fibrillin-1 form a major hyaluronan-binding complex in the ciliary body (2008)
  • Author(s): Ohno-Jinno A, et. al.
  • Journal Title: Invest Ophthalmol Vis Sci. (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of a motif for specific binding to hyaluronan in chicken SPACR (2008)
  • Author(s): Zhao J, et. al.
  • Journal Title: J Neurochem. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Versican, fibrillin-1 form a major hyaluronan-binding complex in the ciliary body (2008)
  • Author(s): Ohno-Jinno A, Isogai Z, Yoneda M, Kasai K, Miyaishi O, Inoue Y, Kataoka T, Zhao J, Li H, Takeyama M, Keene K R, Sakai L Y, Kimata K, Iwaki M, Zako M.
  • Journal Title: Invest Ophthalmol Vis Sci. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of a motif for specific binding to hyaluronan in chicken SPACR. (2008)
  • Author(s): Zhao J, Yoneda M, Takeyama M, Miyaishi O, Inoue Y, Kataoka T, Ohno-Jinno A, Isogai Z, Kimata K, Iwaki M, Zako M.
  • Journal Title: J Neurochem. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chemistry and Biology of Carbohydrates for Health Professionals. Functional Glycosaminoglycans in Eye. (2008)
  • Author(s): Zako M, Yoneda M.
  • Journal Title: Edited by Hari G. Garg, Charles A. Hales. ELSEVIER BV(Amsterdam)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Versican,a major hyaluronan-binding component in the dermis,loses its hyaluronan-binding ability in solar elastosis (2007)
  • Author(s): Hasegawa K, et. al.
  • Journal Title: J Invest Dermatol. 127 Pages: 1657-63
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Versican, a major hyaluronan-binding component in the dermis, loses its hyaluronan-binding ability in solar elastosis. (2007)
  • Author(s): Hasegawa K, Yoneda M, Kuwabara H, Miyaishi O, Itano N, Ohno A, Zako M, Isogai Z.
  • Journal Title: J Invest Dermatol. 127 Pages: 1657-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular cloning and characterization of chick SPACRCAN. (2006)
  • Author(s): Inoue Y, Yoneda M, Zhao J, Miyashi O, Ohno-Jinnno A, Kataoka T, Isogai Z, Kimata K, Iwaki M, Zako M.
  • Journal Title: J Bilo Chem. 281 Pages: 10381-8
• [Journal Article] Expression profile of heat shock protein 108 during retinal development in the chick. (2006)
  • Author(s): Zhaio J, Yoneda M, Inoue Y, Kakizaki H, Ohno-Jinno A, Kataoka T, Iwaki M, Zako M.
  • Journal Title: Neurosci Lerr. 397
• [Journal Article] Versican and fibrillin-1 form a major hyaluronan-binding complex in the ciliary body.
  • Author(s): Ohno-Jinno A, Isogai Z, Yoneda M, Kasai K, Miyaishi O, Inoue Y, Kataoka T, Zhao J, Li H, Takeyama M, Keene K R, Sakai L Y, Kimata K, Iwaki M, Zako M.
  • Journal Title: Invest Ophthalmol Vis Sci. (in press)
  • Peer Reviewed
• [Journal Article] Versican,a major Hyaluronan-Binding Component in the Dermis,Loses its Hymaluronan-Binding Ability in Solar Elastosis.
  • Author(s): Hasegawa K, Yoneda M, Kuwabara H, Miyaishi O, Itano N, Ohno A, Zako M, Isogai Z.
  • Journal Title: J Invest Dermatol. (in press)
• [Presentation] 網膜分化時のニワトリSPACRとSPACRCAN分子の発現比較 (2007)
  • Author(s): 雑喉 正泰
  • Organizer: 第111回日本眼科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Moumakubunnkaji no niwatori SPACR to SPACRCAN bunnshi no hatsugenhikaku. (2007)
  • Author(s): Zako M
  • Organizer: The 111th Annual Meeting of the Japanese Ophthalmological Society.
  • Place of Presentation: Osaka
  • Year and Date: 2007-04-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ニワトリ毛様体と硝子体におけるversican-fibrillin-l-hyaluronan複合体の違い (2007)
  • Author(s): 大野安季子
  • Organizer: 第111回日本眼科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-19
• [Book] Chemistry and Biology of Carbohydrates for Health Professionals. (2008)
  • Author(s): Zako M, and Yoneda M.
  • Total Pages: 432
  • Publisher: ELSEVIER BV
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Functional Glycosaminoglycans in Eye. (2008)
  • Author(s): Zako M, and Yoneda M.
  • Publisher: ELSEVIER BV(Amsterdam)
• [Book] Chemistry and Biology of Carbohydarates for Health professionals.
  • Author(s): Zako M, Yoneda M.
  • Publisher: ELSEVIER BV(Amsterdam)(in press.)