| Project/Area Number |
18591952
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Gifu University |
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Principal Investigator |
MOTOHASHI Tsutomu Gifu University, Graduate School of Medicine, Associate Professor (40334932)
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| Co-Investigator(Kenkyū-buntansha) |
KUNISADA Takahiro Gifu University, Graduate School of Medicine, Professor (30205108)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Neural Crest Cell / Embryonic Stem Cell / Sox10 / Green Fluorescence Protein (GFP) / Cell Sorter / Neurocristopathy |
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| Research Abstract |
The purpose of this research is to analyze the differentiation and mobility of neural crest cells by utilizing the mouse ES cells derived neural crest cell. We established SoxlO-ires-gfp ES cells in order to purify the ES cell derived neural crest like cells. The SoxlO-ires-gfp ES cells have the gene of green fluorescence protein (GFP) after the stop codon of sox10 gene. When the ES cells are differentiated into neural crest cells, the cells express GFP together with Sox10, which is expressed in in vivo neural crest cells, and the ES cell derived neural crest cells are purified by utilizing GFP expression. Using the SoxlO-ires-gfp ES cells, we effectively enriched the ES cell derived neural crest-like cells from culture. Transplantation of the enriched neural crest-like cells into chick embryo suggested that the neural crest-like cells had similar mobility to in vivo neural crest cells. And we analyzed the development of the neural crest-like cells from ES cells. We showed that the neural crest-like cells were categorized into the Sox10+/c-Kit- and the Sox10+/c-Kit+ population, and the Sox10+/c-Kit- population especially was more immature neural crest-like cells compare to the Sox10+/c-Kit+ population.
Furthermore, we established the mice derived from the SoxlO-ires-gfp ES cells. In the embryos, neural crest cells and the derivatives were clearly marked with Green fluorescence protein. The SoxlO-ires-gfp ES cells and the mice derived from the SoxlO-ires-gfp ES cells are thought to be valuable tools for neural crest studies. Neural Crest Cell.
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