In vivo microvascular regeneration in the various conditions and molecular expressions and regulations
| Project/Area Number |
18591967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
TANAKA Katsumi Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor (70244069)
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| Co-Investigator(Kenkyū-buntansha) |
ANRAKU Kuniaki Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (60380846)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | cytokine / optimal microvascular environment / clinical assessment / lower calf wound healing / sensory nerves / micro-angiogenesis / 基礎疾患 / 人工真皮 / 血管化腹壁皮弁 / 間葉系幹細胞 / 血流付加 |
|---|
| Research Abstract |
Obesity, hypertension, atherosclerosis obliterans (ASO)and diabetes mellitus (DM) are increasing as co-morbidities increasing among those who are undertaking free flap surgeries and digital re-plantations. In these co-morbidities, there are remarkable damages in the endothelial cells of these blood vessels and thus induced hypoxia and the oxidized stresses and ischemic-reperfusion injury are highly concerned. Even macroscopically successful re-anastomoses may lead to thrombosis afterward. Thus, the optimal microvascular environment was investigated with low-sensory and low-blood flow models.
In the microvascular models, after ligating the femoral vessels and severing the saphenous nerves, the epigastric fascial flap of which the nutrient vessels are the superficial epigastric artery and veins, covered the ligated-vessels and severed-nerves by wrapping. The human derived mesenchymal stem cells, together with basic fibroblast growth factor (bFGF)were injected through the nutrient artery (the superficial epigastric artery)and then incubated for 10 minutes by clamping after the wrapped fascial flap was attached to the defects. The analysis of the nerve conduction velocity and tissue blood flows was temporally investigated. The ipsi-lateral 4-mm full-thickness paw skin defect was also compared among groups temporally.
Also, clinically the head-and-neck surgery and extremities are sometimes difficult for microvascular surgical reconstructions, because spaspms and the differences of the diameters of the vessels are often experienced. As an alternative reconstructive modalities, especially severely damaged lower legs in the elderly population, the artificial dermis with cytokine (bFGF)were effective in wound coverage. In the analysis of quality of wounds, there is much better scar quality in bFGF-treated wounds from the immediately post-wounding as well as shortening the wound healing time.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Combined surgical excision and radiation therapy for keloid treatment2007
Author(s)
Akita, S., Akino, K., Yakabe, A., Imaizumi, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
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Journal Title
J Craniofac Surg 18
Pages: 1164-1169
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Polyurethane dressing is beneficial for split-thickness skin-graft donor wound healing2006
Author(s)
Akita, S., Akino, K., Iraaizurai, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] The quality of pediatric burn scars is improved by early administration of basic fibroblast growth factor2006
Author(s)
Akita, S., Akino, K., Imaizumi, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
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Journal Title
J Burn Care Res(formerly J Burn Care Rehabil) 27
Pages: 333-338
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Therapeutic choice for craniofacial venous malformations2006
Author(s)
Akita, S., Akino, K., Tanaka, K., Anraku, K., Yano, H., Hirano, A
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Journal Title
J Craniofac Surg 17
Pages: 729-735
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Vascurazied tissue transfer for the reconstruction following the tumor resection of the head and neck cancer involving skull base2007
Author(s)
Tanaka, K., Anraku, K., Sakihama, N., Matsuo, T., Nagata, I., Hirano, A
Organizer
World Society for Rreconstructive Microsurgery,IV Congress
Place of Presentation
Athens Greece
Year and Date
2007-06-24
Description
「研究成果報告書概要(欧文)」より
Related Report
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