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In vivo microvascular regeneration in the various conditions and molecular expressions and regulations

Research Project

Project/Area Number 18591967
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionNagasaki University

Principal Investigator

TANAKA Katsumi  Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor (70244069)

Co-Investigator(Kenkyū-buntansha) ANRAKU Kuniaki  Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (60380846)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordscytokine / optimal microvascular environment / clinical assessment / lower calf wound healing / sensory nerves / micro-angiogenesis / 基礎疾患 / 人工真皮 / 血管化腹壁皮弁 / 間葉系幹細胞 / 血流付加
Research Abstract

Obesity, hypertension, atherosclerosis obliterans (ASO)and diabetes mellitus (DM) are increasing as co-morbidities increasing among those who are undertaking free flap surgeries and digital re-plantations. In these co-morbidities, there are remarkable damages in the endothelial cells of these blood vessels and thus induced hypoxia and the oxidized stresses and ischemic-reperfusion injury are highly concerned. Even macroscopically successful re-anastomoses may lead to thrombosis afterward. Thus, the optimal microvascular environment was investigated with low-sensory and low-blood flow models.
In the microvascular models, after ligating the femoral vessels and severing the saphenous nerves, the epigastric fascial flap of which the nutrient vessels are the superficial epigastric artery and veins, covered the ligated-vessels and severed-nerves by wrapping. The human derived mesenchymal stem cells, together with basic fibroblast growth factor (bFGF)were injected through the nutrient artery (the superficial epigastric artery)and then incubated for 10 minutes by clamping after the wrapped fascial flap was attached to the defects. The analysis of the nerve conduction velocity and tissue blood flows was temporally investigated. The ipsi-lateral 4-mm full-thickness paw skin defect was also compared among groups temporally.
Also, clinically the head-and-neck surgery and extremities are sometimes difficult for microvascular surgical reconstructions, because spaspms and the differences of the diameters of the vessels are often experienced. As an alternative reconstructive modalities, especially severely damaged lower legs in the elderly population, the artificial dermis with cytokine (bFGF)were effective in wound coverage. In the analysis of quality of wounds, there is much better scar quality in bFGF-treated wounds from the immediately post-wounding as well as shortening the wound healing time.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 2 results) Presentation (6 results) Book (1 results) Remarks (2 results)

  • [Journal Article] 各種ドナー部位における採骨のコツと特性、血管柄付骨移植-腸骨2007

    • Author(s)
      田中 克己、平野 明喜
    • Journal Title

      PEPARS 15

      Pages: 10-15

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Combined surgical excision and radiation therapy for keloid treatment2007

    • Author(s)
      Akita, S., Akino, K., Yakabe, A., Imaizumi, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
    • Journal Title

      J Craniofac Surg 18

      Pages: 1164-1169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 植皮、皮弁辺縁部の縫合2007

    • Author(s)
      田中 克己、平野 明喜
    • Journal Title

      PEPARS 14

      Pages: 93-99

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 深部組織損傷を伴った電撃傷2007

    • Author(s)
      田中 克己、近藤 加代子、平野 明喜
    • Journal Title

      救急・集中治療 19

      Pages: 1236-1240

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Cranial Vault Distraction:Its Illusionary Effect and Limitation2006

    • Author(s)
      Yano H, Tanaka K, Sueyoshi O, Takahashi K, Hirata R, Hirano A.
    • Journal Title

      Plast Reconstr Surg 117

      Pages: 193-200

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lower extremity reconstruction after necrotizing fasciitis and necrotic skin lesions using a porcine derived skin substitute2006

    • Author(s)
      Akita S, Tanaka K, Hirano A.
    • Journal Title

      J PIast Reocnstr Aesthet Surg 59

      Pages: 759-763

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sleep disturbances detected by a sleep apnea monitor in craniofacial surgical patients2006

    • Author(s)
      Akita, S., Anraku, K., Tanaka, K., Yano, H., Hirano, A
    • Journal Title

      J Craniofac Surg 17

      Pages: 44-49

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Polyurethane dressing is beneficial for split-thickness skin-graft donor wound healing2006

    • Author(s)
      Akita, S., Akino, K., Iraaizurai, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
    • Journal Title

      Burns 32

      Pages: 447-451

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The quality of pediatric burn scars is improved by early administration of basic fibroblast growth factor2006

    • Author(s)
      Akita, S., Akino, K., Imaizumi, T., Tanaka, K., Anraku, K., Yano, H., Hirano, A
    • Journal Title

      J Burn Care Res(formerly J Burn Care Rehabil) 27

      Pages: 333-338

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lower extremity reconstruction after necrotizing fasciitis and necrotic skin lesions using a porcine-derived skin substitute2006

    • Author(s)
      Akita, S., Tanaka, K., Hirano, A
    • Journal Title

      J Plast Reconstr Aesthet Surg(formerly Br J Plast Surg) 59

      Pages: 759-763

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Therapeutic choice for craniofacial venous malformations2006

    • Author(s)
      Akita, S., Akino, K., Tanaka, K., Anraku, K., Yano, H., Hirano, A
    • Journal Title

      J Craniofac Surg 17

      Pages: 729-735

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 長期経過からみた瘢痕および瘢痕拘縮の問題点とその対策2006

    • Author(s)
      田中克己, 中川浩志, 平野明喜
    • Journal Title

      形成外科 49

      Pages: 899-903

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 重度下肢開放骨折における血管柄付遊離組織移植の適用2006

    • Author(s)
      田中克己, 村上隆一, 安楽邦明, 藤岡正樹, 弦本敏行, 寺本 司, 平野明喜
    • Journal Title

      日本マイクロサージェリー学会誌 19

      Pages: 313-324

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 咽頭喉頭頸部食道摘出術後の再建における多施設共同研究2006

    • Author(s)
      杉山成史, 木股敬裕, 関堂 充, 田中克己 他
    • Journal Title

      頭頚部癌 32

      Pages: 486-493

    • NAID

      10018452818

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cranial Vault Distraction : Its Illusionary Effect and Limitation2006

    • Author(s)
      Yano H, Tanaka K, Sueyoshi O, Takahashi K, Hirata R, Hirano A.
    • Journal Title

      Plast Reconstr Surg 117

      Pages: 193-200

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cranioplasty with auto-purified bone flap after infection2006

    • Author(s)
      Yano H, Tanaka K, Matsuo, T, Tsuda M, Akita S, Hirano A.
    • Journal Title

      J Craniofac Surg 17

      Pages: 1076-1079

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lower extremity reconstruction after necrotizing fasciitis and necrotic skin lesions using a porcine-derived skin substitute.2006

    • Author(s)
      Akita S, Tanaka K, Hirano A.
    • Journal Title

      J Plast Reocnstr Aesthet Surg 59

      Pages: 759-763

    • Related Report
      2006 Annual Research Report
  • [Presentation] Versatility of cheek and cervicofacial flap for reconstruction of cheek defects2007

    • Author(s)
      Tanaka, K., Hirano, A
    • Organizer
      The 17th Japan-China Joint Meeting on Plastic Surgery, Educational lecture
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-10-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vascurazied tissue transfer for the reconstruction following the tumor resection of the head and neck cancer involving skull base2007

    • Author(s)
      Tanaka, K., Anraku, K., Sakihama, N., Matsuo, T., Nagata, I., Hirano, A
    • Organizer
      World Society for Rreconstructive Microsurgery,IV Congress
    • Place of Presentation
      Athens Greece
    • Year and Date
      2007-06-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Limit of free skin graft and application of a free flap for intractable venostatic leg ulcer2007

    • Author(s)
      Tanaka K, Murakami R, Hirano A
    • Organizer
      World Congress of the International Union of Phlebology-Asian Chapter Meetin
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-06-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Limit of free skin graft and application of a free flap for intractable venostatic leg ulcer2007

    • Author(s)
      Tanaka, K., Murakami, R., Hirano, A
    • Organizer
      World Congress of the International Union of Phlebology-Asian Chapter Meeting 2007
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-06-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Limit of free skin graft and application of a free flap for intractable venostatic leg ulcer2007

    • Author(s)
      Tanaka K, Murakami R, Hirano A
    • Organizer
      World Congress of the International Union of Phlebology-Asian Chapter Meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-06-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Extended sigmoid-shaped free jejunal patch for reconstruction of the oral base and pharynx after total glossectoray with laryngectomy2006

    • Author(s)
      Anraku, K., Tanaka, K., Hirano, A
    • Organizer
      The 16th China-Japan Joint Meeting in Plastic Surgery
    • Place of Presentation
      China, Haerbin
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 特殊部位の熱傷、「会陰部・肛門部熱傷」熱傷治療マニュアル2007

    • Author(s)
      田中 克己
    • Total Pages
      419
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.med.nagasaki-u.ac.jp/plastics/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.med.nagasaki-u.ac.jp/plastics/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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