| Project/Area Number |
18591971
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | SHOWA UNIVERSITY |
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Principal Investigator |
BUNSHO Kao SHOWA UNIVERSITY, Medicine, Associate professor (10327893)
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| Co-Investigator(Kenkyū-buntansha) |
HOSAKA Yoshiaki Showa University, Medicine, Professor (40156998)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | ARTIFICIAL SKIN / WOUND HEALING / HUMAN SKIN MODEL / IMMUNOHISTOCHEMICAL TECHNIQUES / PEPTIDE HYDROGEL |
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| Research Abstract |
Artificial skin cultures made using cultured human skin cells have attracted attention as experimental models, as well as for clinical applications. However artificial skin has few chemical articles that undergo damage and observed progress. We created a number of artificial skin models with human immune cells. These artificial skin models were irradiated with a CO2 laser at various strengths. We evaluated the degree of damage and the time course of tissue regeneration in these models using pathological and immunohistochemical techniques.
Also using biocompatible peptide hydrogel as a scaffold, we prepared three -dimensional synthetic skin that does not contain animal-derived materials or pathogens. The present study investigated preparation methods, proliferation, and functional expression of fibroblasts in the synthetic dermis and differentiation of keratinocytes in the epidermis. Synthetic dermis was prepared by mixing fibroblasts with peptide hydrogel, and synthetic skin was prepared by forming an epidermal layer using keratinocytes on the synthetic dermis. A fibroblast-rich foamy layer consisting of homogeneous peptide hydrogel subsequently formed in the synthetic dermis, with fibroblasts aggregating in clusters within the septum. The epidermis consisted of 3-5 keratinocyte layers. Immunohistochemical staining showed human type I collagen, indicating functional expression around fibroblasts in the synthetic dermis, keratinocyte differentiation in the epidermis, and expression of basement membrane proteins. The number of fibroblasts tended to increase up to the second week and was maintained up to the fourth week, but rapidly decreased in the fifth week. In the synthetic dermis medium, the human type I collagen concentration increased after the second week to the fifth week. These findings suggest that peptide hydrogel acts as a synthetic skin scaffold that offers a platform for the proliferation and functional expression of fibroblasts and keratinocytes.
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