| Project/Area Number |
18591988
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SHIME Nobuaki Kyoto Prefectural University of Medicine, Medical school, Associate professor (00260795)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYA Takaaki Osaka University, Medical school, Associate professor (80271633)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Sepsis / Innate immunity / Commensal micorflora / Digestive tract / Antibiotics / Macrophage / 敗血症 / 常在菌巣 |
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| Research Abstract |
We investigated the role of digestive commensal bacteria in the innate immune response in experimental mouse sepsis model. Experimental mice were fed by water including multiple antibiotics (streptomycin and cefotaxime) for three weeks. Significant decreases in the amount of commensal bacterial flora, as well as the changes in intestinal organic acid profiles were noted in the experimental group. The expressions of TLR-4 and TLR-2 receptors in the peritoneal macrophages assessed by flow-cytometry, have been significantly downregulated. The increase in plasma TNF-α level after lipopolysaccharide injection, mimicking clinical sepsis, has been attenuated. This attenuation was accompanied with the increases in the mortality. Those data suggests that commensal bacterial flora in the digestive tract might confer a significant role in modulating innate immune system. The immunomodulating effect of antimicrobials via the changes in commensal microflora should be considered in the sepsis treatment.
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