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Elucidation of molecular mechanism underlying smooth muscle-like differentiation of fibroblastic cells derived from periodontal ligament.

Research Project

Project/Area Number 18592026
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionHokkaido University

Principal Investigator

ISHISAKI Akira  Hokkaido University, Graduate School of Dentistry, Associate Professor (20356439)

Co-Investigator(Kenkyū-buntansha) TAMURA Masato  Hokkaido University, Graduate School of Dentistry, Professor (30236757)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsperiodontal ligament / stem cell / differentiation / tube-like structure
Research Abstract

A blood supply is indispensable for the regeneration of damaged or lost periodontal ligament (PDL) tissue. Mesenchymal stem cell-like activity of cells derived from the PDL has been identified by their capacity to form fibrous and osseous tissue, and cementum. However, it remains to be clarified whether the cells have an ability to build the capillary network of blood vessels. This study evaluated the potential of cells derived from the PDL to construct a blood vessel-like structure, and examined how growth factors controlled the multipotency of the cells.
The ability of a swine PDL fibroblast cell line, TesPDL3, to construct a blood vessel-like structure was evaluated. In addition, the ability of the cells to form mineralized nodules was evaluated on type I collagen-coated plastic plates. In some cases, fibroblast growth factor (FGF)-2 and bone morphogenetic protein (BMP)-2 were added to these cultures. The status of the expression of vascular and osteoblastic cell-specific markers in the cells was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR), western blotting and immunofluorescent analyses.
TesPDL3 cells not only formed mineralized nodules in response to BMP-2 stimulation but also constructed tube-like structures in response to FGF-2 stimulation. Intriguingly, FGF-2 inhibited the BMP-2-induced mineralized nodule-formation. Conversely, BMP-2 inhibited the FGF-2-induced tube-like structure-formation. Thus, PDL fibroblasts have the potential to differentiate not only into osteoblastic but also into vascular cell lineages. The destiny of the cells was reciprocally regulated by BMP-2 and FGF-2.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (19 results)

All 2008 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (12 results)

  • [Journal Article] Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone2008

    • Author(s)
      Kaname Shirai
    • Journal Title

      J. Periodontal Res. 印刷中(未定)(掲載確定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone morphogenetic protein.2008

    • Author(s)
      Kaname Shirai
    • Journal Title

      J. Periodontal Res. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone morphogenetic protein.2008

    • Author(s)
      Kaname Shirai
    • Journal Title

      J. Periodontal Res. 未定(掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.2007

    • Author(s)
      Miho Ibi
    • Journal Title

      Arch. Oral Biol. 52

      Pages: 1002-1008

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.2007

    • Author(s)
      Miho Ibi
    • Journal Title

      Arch. Oral Biol. 52(No-Concerned)

      Pages: 1002-1008

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of p44/p42 MAP kinase in insulin-like growth factor-I-induced alkaline phosphatase activity in osteoblast-like-MC3T3-E1 cells2006

    • Author(s)
      Yoshiteru Hanai, Haruhiko Tokuda, Akira Ishisaki, Rie Matsushita-Nishiwaki, Norimi Nakamura, Minoru Yoshida, Shinji Takai, Toshiki Ohta, Osamu Kozawa
    • Journal Title

      Molecular and Cellular Endocrinology 251・1-2

      Pages: 42-48

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential roles of MAP kinases in atorvastatin-induced VEGF release in cardiac myocyte2006

    • Author(s)
      Keiichi Nakajima, Hidetaka Suga, Hirouyki Matsuno, Akira Ishisaki, Kouseki Hirade, Osamu Kozawa
    • Journal Title

      Life Sciences 79・12

      Pages: 1214-1220

    • Related Report
      2006 Annual Research Report
  • [Presentation] FGF-stimulated formation of tube-like structure by periodontal ligament cells in self-assembling peptide scaffolds.2007

    • Author(s)
      Kaname Shirai
    • Organizer
      93rd Annual Meeting of American Academy of Periodontology
    • Place of Presentation
      ワシントンコンベンションセンター(ワシントンDC)
    • Year and Date
      2007-10-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] FGF-stimulated formation of tube-like structure by periodontal ligament cells in self-assembling peptide scaffolds.2007

    • Author(s)
      Kaname Shirai
    • Organizer
      93^<th> Annual Meeting of American Academy of Periodontology
    • Place of Presentation
      Washington Convention Center (Washington DC)
    • Year and Date
      2007-10-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯根膜由来細胞が有するFGF-2依存的管腔構造物形成能力について2007

    • Author(s)
      白井 要
    • Organizer
      第50回春季日本歯周病学会学術大会
    • Place of Presentation
      横須賀芸術劇場(横須賀)
    • Year and Date
      2007-05-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] FGF-2 induces construction of tube-like structure by fibroblast-like cells derived from periodontal ligament.2007

    • Author(s)
      Kaname Shirai
    • Organizer
      The 50^<th> Annual Meeting of The Japanese Society of Periodontology
    • Place of Presentation
      Yokosuka Arts Theatre (Yokosuka)
    • Year and Date
      2007-05-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯根膜細胞における幹細胞増殖因子による増殖とアルカリフォスファターゼおよびCD31発現の制御2007

    • Author(s)
      大久保 直登
    • Organizer
      第80回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of proliferation and alkaline phosphatase and CD31 gene expression by stem cell factor in a periodontal ligament cells.2007

    • Author(s)
      Naoto Ohkubo
    • Organizer
      The 80^<th> Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Nagoya Congress Center (Nagoya)
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of osteoprotegerin and RANKL gene expression by Wnt and bone morphogenetic protein-2 during osteoblastic differentiation2006

    • Author(s)
      Mari Sato
    • Organizer
      ASBMR 28th Annual Meeting
    • Place of Presentation
      フィラデルフィアコンベンションセンター(フィラデルフィア)
    • Year and Date
      2006-09-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of osteoprotegerin and RANKL gene expression by Wnt and bone morphogenetic protein-2 during osteoblastic differentiation.2006

    • Author(s)
      Mari Sato
    • Organizer
      ASBMR 28^<th> Annual Meeting
    • Place of Presentation
      Philadelphia Convention Center (Philadelphia)
    • Year and Date
      2006-09-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transdifferentiation of periodontal ligament cells into smooth-muscle cells.2006

    • Author(s)
      Miho Ibi
    • Organizer
      84th General Session of the IADR
    • Place of Presentation
      ブリスベンコンベンション展示センター(ブリスベン)
    • Year and Date
      2006-06-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biological effects of self-assembling short-peptide scaffold on periodontal ligament regeneration2006

    • Author(s)
      Kaname Shirai
    • Organizer
      84th General Session of the IADR
    • Place of Presentation
      ブリスベンコンベンション展示センター(ブリスベン)
    • Year and Date
      2006-06-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transdifferentiation of periodontal ligament cells into smooth-muscle cells.2006

    • Author(s)
      Miho Ibi
    • Organizer
      84^<th> General Session of the IADR
    • Place of Presentation
      Brisben Convention Center (Brisben)
    • Year and Date
      2006-06-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biological effects of self-assembling short-peptide scaffold on periodontal ligament regeneration.2006

    • Author(s)
      Kaname Shirai
    • Organizer
      84^<th> General Session of the IADR
    • Place of Presentation
      Brisben Convention Center (Brisben)
    • Year and Date
      2006-06-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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