| Project/Area Number |
18592037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
IRIFUNE Masahiro Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor (10176521)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAHARA Michio Hiroshima University, Graduate School of Biomedical Sciences, Professor (30034094)
DOHI Toshihiro Hiroshima University, Graduate School of Biomedical Sciences, Professor (00034182)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | General Anesthesia / Behavioral pharmacology / μ-Opioid receptor agonist / α_2-Adrenergic receptor agonist / Glycine transporter inhibitor / Unconsciousness / Immobility / Analgesia / α2-アドレナリン受容体作動薬 / 全身麻痺作用 |
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| Research Abstract |
The role of μ-opioid, α_2-adrenergic, and glycine receptors in general anesthetic action was examined in mice. All drugs were administered intraperitoneally. General anesthetic action was evaluated using three end points: 1) loss of righting reflex (LORR; as a measure of unconsciousness), 2) loss of movement in response to noxious stimulation (as a measure of immobility), and 3) loss of nociceptive response (as a measure of analgesia). Gabaculine, which is a γ-aminobutyric acid (GABA)-transaminase inhibitor that increases endogenous GABA in the brain, dose-dependently induced LORR. However, even a large dose produced no immobility. Both the μ-opioid receptor agonist morphine and the α_2-adrenergic receptor agonist dexmedetomidine dose-dependently induced analgesia. In addition, both morphine and dexmedetomidine in combination with gabaculine produced immobility in a dose-dependent fashion. However, glycine transporter inhibitors did not affect immobility. These findings suggest that LORR is associated with enhanced GABA activity, and immobility is involved in μ-opioid and α_2-adrenergic receptors, but not glycine receptors.
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