Function of endo-lysosomal proteinases in epithelial cells

• Project/Area Number: 18592038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University (2007); Kyushu University (2006)
• Principal Investigator: TSUKUBA Takayuki Nagasaki University, Department of Pathopharmacology, Professor (30264055)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Kenji Nagasaki University, Department of Pharmacology Graduate School of Dental Science, Professor (40091326) ; KADOWAKI Tomoko Nagasaki University, Department of Pharmacology Graduate School of Dental Science, Assistant Professor (70336080)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: cathepsin / endosomes / lysosomes / epithelial cells / deficient mice / skin / gingiva / protease

Research Abstract

Epithelial cells are thought to have several defense mechanisms by cornification against stimulation, stress and infection. Among them, gingival epithelial cells are major cells in oral cavity, which consist of keratinizing squamous cells. Recently, a new important gene regulating host defense in epithelial cells has been identified. It is so called "cathepsin". Cathepsin is a family name of protainases localized in endosomes and/or lysosomes. For example, it is known that cathepsin C is the responsible gene for Papillon-Lefevre syndrome, which is characterized by severe periodontitis and palmoplantar keratoderma. Moreover, deficient mice for cathepsin L exhibit epidermal hyperplasia due to a hyper-proliferation of basal keratinocytes. On the other hand, to investigate the physiological role of the endo-lysosomal aspartic proteinase cathepsin E, we have generated cathepsin E-deficient mice. They spontaneously developed atopic dermatitis-like skin lesions with thickened epidermis. Furthermore, cathepsin E-deficient mice showed a cancer on epithelial cells in a different genetic background. These results suggest the possibility that cathepsin E-deficient mice show abnormalities not only in skin epithelial cells, but also in gingival epithelial cells. However, the molecular mechanisms of cathepsin E deficiency remain to be determined. In this study, we analyzed physiological and/or pathological functions of cathepsin E in epithelial cells including the skin and gingiva. Our final goal is application for the development of diagnosis and therapeutic agents targeting at these cathepsins.

Research Products

• [Journal Article] Association of cathepsin E deficiency with the increased territorial aggressive response of mice (2008)
  • Author(s): Shigematsu N, Fukuda T, Yamamoto T, Nishioku T, Yamaguchi T, Himeno M, Nakayama. KI, Tsukuba T, Kadowaki T, Okamoto K, Higuchi S, Yamamoto K.
  • Journal Title: J Neurochem. 105 Pages: 1394-1404
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Determination of active site of lysine-specific cysteine proteinase(Lys-gingipain)by use of a Porphyromonas gingivalis plasmid system (2008)
  • Author(s): Ishida Y, Hu J, Sakai E, Kadowaki T, Yamamoto K, Tsukuba T, Kato Y, Nakayama K, Okamoto K.
  • Journal Title: Arch Oral Biol. 53 Pages: 538-544
  • NAID: 120006984583
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Dentin phosphophoryn promotes cellular migration of human dental pulp cells (2008)
  • Author(s): Yasuda Y, Izumikawa M, Okamoto K, Tsukuba T, Saito T
  • Journal Title: J Endodont 34 Pages: 575-578
  • NAID: 120006981302
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Gene expression profiling of mammary elands of cathepsin E-deficient mice compared with wild-type littermates (2008)
  • Author(s): Kawakubo, T, Yasukochi, A, Tsukuba, T, Kadowaki, T, Yamamoto, K
  • Journal Title: Biochimie 90 Pages: 396-404
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Berberine inhibits RANKL-induced osteoclast formation and survival through suppressing the NF-kappaB and Akt pathways (2008)
  • Author(s): Hu, JP, Nishishita, K, Sakai, E, Yoshida, H, Kato, Y, Tsukuba, T, Okamoto, K
  • Journal Title: Eur J Pharmacol 580 Pages: 70-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association of cathepsin E deficiency with the increased territorial aggressive response of mice (2008)
  • Author(s): Shigematsu, N, Fukuda, T, Yamamoto, T, Nishioku, T, Yamaguchi, T, Himeno, M, Nakayama, KI, Tsukuba, T, Kadowaki, T, Okamoto, K, Higuchi, S, Yamamoto, K
  • Journal Title: J Neurochem (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Determination of active site of lysine-specific cysteine proteinase(Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system (2008)
  • Author(s): Y, Hu, J, Sakai, E, Kadowaki, T, Yamamoto, K, Tsukuba, T, Kato, Y, Nakayama, K, Okamoto, K
  • Journal Title: Arch Oral Biol (in press)
  • NAID: 120006984583
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dentin phosphophoryn promotes cellular migration of human dental pulp cells (2008)
  • Author(s): Ishida, Yasuda, Y, Izumikawa, M, Okamoto, K, Tsukuba, T, Saito, T
  • Journal Title: J Endodont (in press)
  • NAID: 120006981302
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene expression profiling of mammary glands of cathepsin E-deficinet mice compared with wild-type littersmates (2008)
  • Author(s): Kawakubo T, et. al.
  • Journal Title: Biochimie 90 Pages: 396-404
  • Peer Reviewed
• [Journal Article] Berberine inhibits RANKL-induced osteoclast formation and survival through suppressing the NF-kappaB and Akt pathways (2008)
  • Author(s): Hu JP, et. al.
  • Journal Title: Eur J Pharmacol 580 Pages: 70-79
  • Peer Reviewed
• [Journal Article] The role for Gingipains in Porphyromonas gingivalis traffic to phagol ysosomes and survival in human aortic endothelial cells (2007)
  • Author(s): Yamatake K., Maeda M., Kadowaki T., Takii, R., Tsukuba T., Ueno T., Kominami, E., Yokota S., Yamamoto K.
  • Journal Title: Infect.Immun 75 Pages: 2090-2100
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation (2007)
  • Author(s): Danjo A, Yamaza T, Kido MA, Shimohira D, Tsukuba T, Kagiya T, Yamashita Y, Nishijima K, Masuko S, Goto M, Tanaka T.
  • Journal Title: Biochem Biophys Res Commun 360 Pages: 199-204
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E (2007)
  • Author(s): Kakehashi H, Nishioku T, Tsukuba T, Kadowaki T, Nakamura S, Yamamoto K.
  • Journal Title: J Immunol. 179 Pages: 5728-5737
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Association of cathepsin E with tumor growth arrest through angiogenesis inhibition and enhanced immune responses (2007)
  • Author(s): Shin M, Kadowaki T, Iwata J, Kawakubo T, Yamaguchi N, Takii R, Tsukuba T, Yamamoto K.
  • Journal Title: Biol Chem. 388 Pages: 1173-1181
  • NAID: 120006900037
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface (2007)
  • Author(s): Kawakubo T, Okamoto K, Iwata J, Shin M, Okamoto Y, Yasukochi A, Nakayama KI, Kadowaki T, Tsukuba T, Yamamoto K.
  • Journal Title: Cancer Res. 67 Pages: 10869-10878
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Selection-by-function:efficient enrichment of cathepsin E inhibitors from a DNA library (2007)
  • Author(s): Naimuddin M, Kitamura K, Kinoshita Y, Honda-Takahashi Y, Murakami M, Ito M, Yamamoto K, Hanada K, Husimi Y, Nishigaki K.
  • Journal Title: J Mol Recognit. 20 Pages: 58-68
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis,identification of its target protein on erythrocytes,and inhibition of hemagglutination by an interdomain regional peptide (2007)
  • Author(s): Sakai E, Naito M, Sato K, Hotokezaka H, Kadowaki T, Kamaguchi A, Yamamoto K, Okamoto K, Nakayama K.
  • Journal Title: J Bacteriol. 189 Pages: 3977-3986
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Squamous cell carcinoma antigen 1 is an inhibitor of parasite-derived cysteine proteases (2007)
  • Author(s): Kanaji S, Tanaka Y, Sakata Y, Takeshita K, Arima K, Ohta S, Hansell EJ, Caffrey C, Mottram JC, Lowther J, Donnelly S, Stack C, Kadowaki T, Yamamoto K, McKerrow JH, Dalton JP, Coombs GH, Izuhara K.
  • Journal Title: FEBS Lett. 581 Pages: 4260-4264
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A role for gingipains in cellular responses and bacterial survival in Porphyromonas gingivalis-infected cells (2007)
  • Author(s): Kadowaki T, Takii R, Yamatake K, Kawakubo T, Tsukuba T, Yamamoto K
  • Journal Title: Front Biosci 12 Pages: 4800-4809
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Gene expression profiling of mammary glands of cathepsin E-deficient mice compared with wild-type littermates (2007)
  • Author(s): Kawakubo T, Yasukochi A, Tsukuba T, Kadowaki T, Yamamoto K.
  • Journal Title: Biochimie. 90 Pages: 396-404
  • NAID: 120006900027
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cathepsin E deficiency induces a novel form of lysosomal storage disorder in macrophages showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH. *Both authors contributed equally to this work (2007)
  • Author(s): Yanagawa, M*, Tsukuba, T*, Nishioku, T, Okamoto, Y, Okamoto, K, Takii, R, Terada, Y, Nakayama, KI, Kadowaki, T, Yamamoto, K
  • Journal Title: J. Biol Chem 282 Pages: 1851-1862
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role for Gingipains in Porphyromonas gingivalis traffic to phagolysosomes and survival in human aortic endothelial cells (2007)
  • Author(s): Yamatake, K, Maeda, M, Kadowaki, T, Takii, R, Tsukuba, T, Ueno, T, Kominami, E, Yokota, S, Yamamoto, K
  • Journal Title: Infect Immun 75 Pages: 2090-2100
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation (2007)
  • Author(s): Danjo, A, Yamaza, T, Kido, MA, Shimohira, D, Tsukuba, T, Kagiya, T, Yamashita, Y, Nishijima, K, Masuko, S, Goto, M, Tanaka, T
  • Journal Title: Biochem Biophys Res Commun 360 Pages: 199-204
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E (2007)
  • Author(s): Kakehashi, H, Nishioku, T, Tsukuba, T, Kadowaki, T, Nakamura, S, Yamamoto, K
  • Journal Title: J Immunol 179 Pages: 5728-5737
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association of cathepsin E with tumor growth arrest through angiogenesis inhibition and enhanced immune responses (2007)
  • Author(s): Shin, M, Kadowaki, T, Iwata, J, Kawakubo, T, Yamaguchi, N, Takii, R, Tsukuba, T, Yamamoto, K
  • Journal Title: Biol Chem 388 Pages: 1173-1181
  • NAID: 120006900037
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface (2007)
  • Author(s): Kawakubo, T, Okamoto, K, Iwata, J, Shin, M, Okamoto, Y, Yasukochi, A, Nakayama, KI, Kadowaki, T, Tsukuba, T, Yamamoto, K
  • Journal Title: Cancer Res 67 Pages: 10869-10878
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selection-by-function : efficient enrichment of cathepsin E inhibitors from a DNA library (2007)
  • Author(s): Naimuddin, M, Kitamura, K, Kinoshita, Y, Honda-Takahashi, Y, Murakami, M, Ito, M, Yamamoto, K, Hanada, K, Husimi, Y, Nishigaki, K
  • Journal Title: J Mol Recognit 20 Pages: 58-68
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide (2007)
  • Author(s): Sakai, E, Naito, M, Sato, K, Hotokezaka, H, Kadowaki, T, Kamaguchi, A, Yamamoto, K, Okamoto, K, Nakayama, K
  • Journal Title: J Bacteriol 189 Pages: 3977-3986
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Squamous cell carcinoma antigen 1 is an inhibitor of parasite-derived cysteine proteases (2007)
  • Author(s): Kanaji, S, Tanaka, Y, Sakata, Y, Takeshita, K, Arima, K, Ohta, S, Hansell, EJ, Caffrey, C, Mottram, JC, Lowther, J, Donnelly, S, Stack, C, Kadowaki, T, Yamamoto, K, McKerrow, JH, Dalton, JP, Coombs, GH, Izuhara, K
  • Journal Title: FEBS Lett 581 Pages: 4260-4264
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A role for gingipains in cellular responses and bacterial survival in Porphyromonas gingivalis-infected cells (2007)
  • Author(s): Kadowaki, T, Takii, R, Yamatake, K, Kawakubo, T, Tsukuba, T, Yamamoto, K
  • Journal Title: Front Biosci 12 Pages: 4800-48009
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cathepsin E deficiency induces a novel form of lysosomal storage disorder in macrophages showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH (2007)
  • Author(s): Yanagawa M, Tsukuba T, et. al.
  • Journal Title: J.Biol.Chem. 282 Pages: 1851-1862
  • Peer Reviewed
• [Journal Article] The role for Gingipains in Porphyromonas gingivalis traffic to phagolysosomes and survival in human aortic endothelial cells (2007)
  • Author(s): Yamatake K., et. al.
  • Journal Title: Infect.Immun 75 Pages: 2090-2100
  • Peer Reviewed
• [Journal Article] Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation (2007)
  • Author(s): Danjo A, et. al.
  • Journal Title: Biochem Biophys Res Commun. 360 Pages: 199-204
  • Peer Reviewed
• [Journal Article] Differnetial regulation of the nature and functions of dendritic cells and macrophages by cathepsin E (2007)
  • Author(s): Kakehashi H, et. al.
  • Journal Title: J Immunol 179 Pages: 5728-5737
  • Peer Reviewed
• [Journal Article] Association of cathepsin E with tumor growth arrest through angiogenesis inhibition and enhanced immune responses (2007)
  • Author(s): Shin M, et. al.
  • Journal Title: Biol Chem 388 Pages: 1173-1181
  • Peer Reviewed
• [Journal Article] Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface (2007)
  • Author(s): Kawakubo T, et. al.
  • Journal Title: Cancer Res 67 Pages: 10869-10878
  • Peer Reviewed
• [Journal Article] The role for Gingipains in Porphyromonas gingivalis traffic to phagolysosomes and survival in human aortic endothelial cells. (2007)
  • Author(s): Yamatake K., Maeda M et al.
  • Journal Title: Infect. Immun 印刷中
• [Journal Article] Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation expressed in human embryonic kidney 293T cells (2006)
  • Author(s): Tsukuba T., Ikeda S., Okamoto K. Yasuda Y, Sakai E, Kadowaki T., Sakai H., Yamamoto K.
  • Journal Title: FEBS J. 273 Pages: 219-229
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Up-regulation,and enhanced maturation and secretion of cathepsin E in mouse macrophages treated with interferon-y or lipopolysaccharide (2006)
  • Author(s): Yanagawa M., Tsukuba T, Okamoto K., Terada Y, Kadowaki T, Yamamoto K
  • Journal Title: J.Oral Biosci. 48 Pages: 218-225
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cathepsin E-deficient mice show increased susceptibility to bacterial infection associated with the decreased expression of multiple cell surface Toll-like receptors (2006)
  • Author(s): Tsukuba T., Yamamoto S., Yanagawa M., Okamoto K, Okamoto K., Nakayama KI, Kadowaki T, Yamamoto K.
  • Journal Title: J.Biochem. 140 Pages: 57-66
  • NAID: 10018843713
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Selective proteolysis of apolipoprotein B-100 by Arg-gingipain mediates atherosclerosis progression accelerated by bacterial exposure (2006)
  • Author(s): Hashimoto M, Kadowaki T, Tsukuba T., Yamamoto K.
  • Journal Title: J.Biochem. 140 Pages: 713-723
  • NAID: 10018841154
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase (2006)
  • Author(s): Naito M, Sakai E, Shi Y, Ideguchi H, Shoji M, Ohara N, Yamamoto K, Nakayama K.
  • Journal Title: Mel Microbiol. 59 Pages: 152-67
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Novel non-uniform distribution of serotonin transporter in the mouse hippocampus and neocortex revealed by N-and C-terminal domain-specific immunohistochemistry (2006)
  • Author(s): Shigematsu N, Yamamoto K, Higuchi S, Fukuda T.
  • Journal Title: Brain Res. 1075 Pages: 110-116
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Gingipains inactivate a cell surface ligand on Porphyromonas gingival is that induces TLR2-and TLR4-independent signaling (2006)
  • Author(s): Kishimoto M, Yoshimura A, Naito M, Okamoto K, Yamamoto K, Golenbock DT, Hara Y, Nakayama K.
  • Journal Title: Microbiol Immunol. 50 Pages: 315-325
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cathepsin E deficiency induces a novel form of lysosomal storage disorder in macrophages showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH (2006)
  • Author(s): Yanagawa M^*., Tsukuba T^*., Nishioku T, Okamoto Y. Okamoto K., Takii R., Terada Y, Nakayama KI., Kadowaki T, Yamamoto K.
  • Journal Title: J.Biol Chem. 282 Pages: 1851-1862
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Berberine inhibits RANKL-induced osteoclast formation and survival through suppressing the NF-kappaB and Akt pathways (2006)
  • Author(s): Hu JP, Nishishita K, Sakai E, Yoshida H, Kato Y, Tsukuba T, Okamoto K.
  • Journal Title: Eur J Pharmacol. 580 Pages: 70-79
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycoSylation expressed in human embryonic kidney 293T cells (2006)
  • Author(s): Tsukuba, T, Ikeda, S, Okamoto, K, Yasuda, Y, Sakai, E, Kadowaki, T, Sakai, H, Yamamoto, K
  • Journal Title: FEBS J 273 Pages: 219-229
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Up-regulation, and enhanced maturation and secretion of cathepsin E in mouse macrophages treated with interferon-γ or lipopolysaccharide (2006)
  • Author(s): Yanagawa, M, Tsukuba, T, Okamoto, K, Terada, Y, Kadowaki, T, Yamamoto, K
  • Journal Title: J. Oral Biosci 48 Pages: 218-225
  • NAID: 10018041983
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cathepsin E-deficient mice show increased susceptibility to bacterial infection associated with the decreased expression of multiple cell surface Toll-like receptors (2006)
  • Author(s): Tsukuba, T, Yamamoto, S, Yanagawa, M, Okamoto, K, Okamoto, K, Nakayama, KI, Kadowaki, T, Yamamoto, K
  • Journal Title: J. Biochem 140 Pages: 57-66
  • NAID: 10018843713
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selective proteolysis of apolipoprotein B-100 by Arg-gingipain mediates atherosclerosis progression accelerated by bacterial exposure (2006)
  • Author(s): Hashimoto, M, Kadowaki, T, Tsukuba, T, Yamamoto, K
  • Journal Title: J. Biochein 140 Pages: 713-723
  • NAID: 10018841154
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase (2006)
  • Author(s): Naito, M, Sakai, E, Shi, Y, Ideguchi, H, Shoji, M, Ohara, N, Yamamoto, K, Nakayama, K
  • Journal Title: Mol Microbiol 59 Pages: 152-67
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Novel non-uniform distribution of serotonin transporter in the mouse hippocampus and neocortex revealed by N- and C-terminal domain-specific immunohistochemistry (2006)
  • Author(s): Shigematsu, N, Yamamoto, K, Higuchi, S, Fukuda, T
  • Journal Title: Brain Res 1075 Pages: 110-116
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gingipains inactivate a cell surface ligand on Porphyromonas gingivalis that induces TLR2-and TLR4-independent signaling (2006)
  • Author(s): Kishimoto, M, Yoshimura, A, Naito, M, Okamoto, K, Yamamoto, K, Golenbock, DT, Hara, Y, Nakayama, K
  • Journal Title: Microbiol Immunol 50 Pages: 315-325
  • NAID: 10018119237
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation expressed in human embryonic kidney 293T cells. (2006)
  • Author(s): Tsukuba T., Ikeda S., et al.
  • Journal Title: FEBS J. 273巻 Pages: 216-229
• [Journal Article] Up-regulation, and enhanced maturation and secretion of cathepsin E in mouse macrophages treated with interferon-g or lipopolysaccharide. (2006)
  • Author(s): Yanagawa M, Tsukuba T et al.
  • Journal Title: J. Oral Biosci. 48巻 Pages: 218-225
• [Journal Article] Cathepsin E-deficient mice show increased susceptibility to bacterial infection associated with the decreased expression of multiple cell surface Toll-like receptors. (2006)
  • Author(s): Tsukuba T., Yamamoto S., et al.
  • Journal Title: J. Biochem. 140巻 Pages: 57-66
  • NAID: 10018843713
• [Journal Article] Selective proteolysis of apolipoprotein B-100 by Arg-gingipain mediates atherosclerosis progression accelerated by bacterial exposure. (2006)
  • Author(s): Hashimoto M, Kadowaki T, et al.
  • Journal Title: J. Biochem. 140巻 Pages: 713-723
• [Journal Article] Cathepsin E deficiency induces a novel form of lysosomal storage disorder in macrophages showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH. (2006)
  • Author(s): Yanagawa M, Tsukuba T. et al.
  • Journal Title: J. Biol Chem. 282巻 Pages: 1851-1862
• [Presentation] 血管新生抑制と生体防御増強によるカテプシンEの腫瘍抑制 (2007)
  • Author(s): 進正 史, 他
  • Organizer: 第60回日本薬理学会西南部会
  • Place of Presentation: 宮崎・ファニックスシーガイア・コンベンションセンター
  • Year and Date: 2007-11-22
• [Presentation] Roles of cathepsin E in immune responses mediated by macrophages and dendritic cells (2007)
  • Author(s): Yamamoto K., Tsukuba T., Yanagawa M., Kakehashi H., Kawakubo T., Kadowaki T
  • Organizer: 10th International Symposium on Protease Inhibitor and Biological Control
  • Place of Presentation: Portoroz,Slovenia
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The role of cathepsin E in immune responses mediated by macrop hages (2007)
  • Author(s): Yamamoto K.
  • Organizer: Special lecture for Windeyer Institute of Medical Sciences
  • Place of Presentation: Devision of Infection & Immunity,University College of London
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ASSOCIATION OF CATHEPSIN E WITH HOST DEFFENCE A GAINST TUMOR CELLS,(Symposium) (2007)
  • Author(s): Kawakubo T, Yasukochi A, Okamoto K, Y Okamoto Y, Kadowaki T, Tsukuba T, Yamamoto K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Patras,Greece
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles for gingipains in atherosclerosis progression accelerated by bacterial exposure (2007)
  • Author(s): Kadowaki T, Takii R, Hashimoto M, Yamatake K, Tsukuba T, Yamamoto K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Patras,Greece
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] P.gingivalis感染による生活習慣病増悪に関する基礎的研究 (2007)
  • Author(s): 門脇 知子, 瀧井 良祐, 重松 直樹, 筑波 隆幸, 山本 健二
  • Organizer: 第2回口腔健康科学シンポジウム
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cathepsin E mediates membrane trafficking in macrophages (2007)
  • Author(s): Tsukuba T, Yamamoto S, Yanagawa M, Okamoto Y, Okamoto K, Yamamoto K
  • Organizer: 第80回日本薬理学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cathepsin E exerts tumoricidal activity through generation of soluble TRAIL from the tumor cell surface (2007)
  • Author(s): Kawakubo T, Kadowaki T, Tsukuba T, Yamamoto K
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 歯周病と動脈硬化 (2007)
  • Author(s): 山本 健二
  • Organizer: 平成19年度博多区医師会学術講演会特別講演
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 歯周病研究の最前線-病態解明・治療を目指して (2007)
  • Author(s): 山本 健二
  • Organizer: 平成19年度福岡市歯科医師会学術講演会特別講演
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] エンドソーム系におけるカテプシンEの新たな生理機能と作用機序 (2007)
  • Author(s): 筑波 隆幸
  • Organizer: 第12回病態と治療におけるプロテアーゼとインヒビター学会(シンポジウム)
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 成熟破骨細胞の延命と脂質ラフト形成におけるリセドロネートおよびD-PDMPの作用 (2007)
  • Author(s): 坂井 詠子, 福本 敏, 胡 錦萍, 西下 一久, 佛坂 斉祉, 岡元 邦彰, 筑波 隆幸
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ベルベリンによる破骨細胞生存の抑制機構の解明 (2007)
  • Author(s): 胡 錦萍, 西下 一久, 坂井 詠子, 岡元 邦彰, 筑波 隆幸
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カテプシンE欠損によるミトコンドリア機能異常と酸化ストレス亢進 (2007)
  • Author(s): 筑波 隆幸, 柳川 三千代, 岡元 邦彰, 門脇 知子, 山本 健二
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] P.gingivalis感染とインスリン抵抗性に関する実験的解析 (2007)
  • Author(s): 門脇 知子, 瀧井 良祐, 筑波 隆幸, 岡元 邦彰, 山本 健二
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 赤血球におけるカテプシンEの役割 (2007)
  • Author(s): 岡元 邦彰, 石田 豊, 坂井 詠子, 西下 一久, 山本 健二, 筑波 隆幸
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 血管新生抑制と生体防御能増強によるカテプシンEの腫瘍抑制 (2007)
  • Author(s): 進 正史, 門脇 知子, 川久保 友世, 瀧井 良祐, 筑波 隆幸, 山本 健二
  • Organizer: 第60回日本薬理学会西南部会
  • Place of Presentation: 宮崎
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カテプシンE欠損はマクロファージの膜蛋白質輸送異常とミトコンドリア機能異常を起こす (2007)
  • Author(s): 筑波 隆幸, 柳川 三千代, 岡元 邦彰, 岡本 美子, 門脇 知子, 山本 健二
  • Organizer: 第30回日本分子生物学会/第80回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CONTROL OF THE ENDOSOME/LYSOSOME SYSTEM OF MACR OPHAGES BY CATHEPSIN E (2007)
  • Author(s): Tsukuba T, Okamoto K, Kadowaki K, Yamamoto K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Patras,Greece
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 「研究成果報告書概要(欧文)」より (2007)
  • Author(s): Yamamoto, K, Tsukuba, T, Yanagawa, M, kakehashi, H, Kawakubo, T, Kadowaki, T
  • Organizer: 10th International Symposium on Protease Inhibitor and Biological Control
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] The role of cathepsin E in immune responses mediated by macrophages (2007)
  • Author(s): Yainamoto, K
  • Organizer: Special lecture for Windeyer Institute of Medical Sciences, Devision of Infection & Immunity
  • Place of Presentation: University College of London
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ASSOCIATION OF CATHEPSIN E WITH HOST DEFFENCE AGAINST TUMOR CELLS.(Symposium (2007)
  • Author(s): Kawakubo, T, Yasukochi, A, Okamoto, K, Y, Okamoto, Y, Kadowaki, T, Tsukuba, T, Yamamoto, K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Greece
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] CONTROL OF THE ENDOSOME/LYSOSOME SYSTEM OF MACROPHAGES BY CATHEPSIN E (2007)
  • Author(s): Tsukuba, T, Okamoto, K, Kadowaki, K, Yamamoto, K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Greece
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles for gingipains in atherosclerosis progression accelerated by bacterial exposure (2007)
  • Author(s): Kadowaki, T, Takii, R, Hashimoto, M, Yamatake, K, Tsukuba, T, Yamamoto, K
  • Organizer: 5TH General Meeting of the International Proteolysis Society
  • Place of Presentation: Greece
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cathepsin E exerts tumorcidal activity through generation of soluble TRAIL from the tumor cell surface (2007)
  • Author(s): Kawakubo, et. al.
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡・福岡国際会議場
• [Presentation] カテプシンE欠損によるミトコンドリア機能異常と酸化ストレス亢進 (2007)
  • Author(s): 筑波 隆幸, 他
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌・北海道大学
• [Presentation] P.gingivalis感染とインスリン抵抗性に関する実験的解析 (2007)
  • Author(s): 門脇 知子, 他
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌・北海道大学
• [Presentation] 赤血球におけるカテプシンEの役割 (2007)
  • Author(s): 岡元 邦彰, 他
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌・北海道大学
• [Presentation] ベルベリンによる破骨細胞生存の抑制機構の解明 (2007)
  • Author(s): 胡 錦萍, 他
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌・北海道大学
• [Presentation] カテプシンE欠損はマクロファージの膜蛋白質輸送異常とミトコンドリア機能異常を起こす (2007)
  • Author(s): 筑波 隆幸, 他
  • Organizer: 第30回日本会子生物学会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜・パシフィコ横浜
• [Presentation] Roles of cathepsin E in immune responses mediated by macrophages.(Symposium) (2006)
  • Author(s): Tsukuba, T, Yanagawa, M., Yamamoto S., Kadowaki T., Yamamoto K.
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors, Satellite Meeting of the 20th IUBMB Congress
  • Place of Presentation: Awaji,Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Suppression of carcinogenesis and tumor growth by cathepsin E (2006)
  • Author(s): Kawakubo T, Okamoto K., Okamoto Y., Tsukuba, T, Yamamoto K
  • Organizer: the 20th IUBMB Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congres
  • Place of Presentation: Kyoto Japan.
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cathepsin E acts as an inhibitor of tumor cells (2006)
  • Author(s): Shin M., Kadowaki T., Kawakubo T, Tsukuba, T, Yamamoto K
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors
  • Place of Presentation: Awaji,Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A possible mechanism for cathepsin-E mediated tumor suppression (2006)
  • Author(s): Kawakubo T, Okamoto K., Okamoto Y., Tsukuba, T, Yamamoto K
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors
  • Place of Presentation: Awaji,Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カテプシンE欠損マクロファージにおける膜タンパク質の輸送異常 (2006)
  • Author(s): 筑波 隆幸、山本 晋也、柳川 三千代、岡元 邦彰、門脇 知子、山本 健二
  • Organizer: 第48回歯科基礎医学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Porphyromonas gingivalisの生活習慣病増悪機構について (2006)
  • Author(s): 門脇 知子、筑波 隆幸、瀧井 良佑、重松 直樹、岡元 邦彰、山本 健二
  • Organizer: 第48回歯科基礎医学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カテプシンEの過剰発現による腫瘍抑制効果 (2006)
  • Author(s): 進 正史、門脇 知子、川久保 友世、筑波 隆幸、山本 健二
  • Organizer: 第59回日本薬理学会西南部会
  • Place of Presentation: 那覇
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of cathepsin E in immune responses mediated by macrophages.(Symnosium) (2006)
  • Author(s): Tsukuba, T, Yanagawa, M, Yamamoto, S, Kadowaki, T, Yamamoto, K
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors, Satellite Meeting of the 20th IUBMB Congress
  • Place of Presentation: Awaji, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Suppression of carcinogennsis and tumor growth by cathepsin E (2006)
  • Author(s): Kawakubo, T, Okamoto, K, Okamoto, Y, Tsukuba, T, Yamamoto, K
  • Organizer: 20th IUBMB Congress of Biochemistry and Molecular Biology and FAOBMB Congress
  • Place of Presentation: Kyoto Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cathepsin E acts as an inhibitor of tumor cells (2006)
  • Author(s): Shin, M, Kadowaki, T, Kawakubo, T, Tsukuba, T, Yamamoto, K
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors
  • Place of Presentation: Awaji, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A possible mechanism for cathepsin-E mediated tumor suppression (2006)
  • Author(s): Kawakubo, T, Okamoto, K, Okamoto, Y, Tsukuba, T, Yamamoto, K
  • Organizer: The International Symposium on Medical and Biological Perspectives in Proteases and Their Inhibitors
  • Place of Presentation: Awaii, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 12.研究成果による産業財産権の出願・取得状況はないので省略した