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Study on the roles of dendritic cells in inflammatory bone destruction

Research Project

Project/Area Number 18592047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

TAKAMI Masainichi  Showa University, School of Dentistry, Lecturer (80307058)

Co-Investigator(Kenkyū-buntansha) KAMIJO Ryutaro  Showa University, School ofDentistry, Professor (70233939)
YAMADA Atsushi  Showa University, School of Dentistry, Lecturer (50407558)
SUZAWA Tetsuo  Showa University, School of Dentistry, Lecturer (60271285)
MIYAMOTO Yoichi  Showa University, School of Dentistry, Associate Professor (20295132)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsBone metabolism / Innate immunity / Osteoclasts / Transcription factor / Gene expression / Cellular differentiation / Dendritic cells / Teeth / 免疫 / 骨破壊 / 遺伝子 / 炎症
Research Abstract

Severe bone destruction is often induced by enhanced osteoclast formation under the inflammatory conditions. Therefore, elucidation of the mechanism of osteoclastogenesis will contribute to the development of methods for the treatment of diseases that accompanies bone destruction. Monocytes and macrophages are known to differentiate into not only osteoclasts but also dendritic cells that play roles of immune responses. We analyzed the changes of cellular characteristics during osteoclastogenesis and dendritic cell differentiation from common precursors. It was found that the potential of dendritic cell differentiation was lost within 24h after beginning of osteoclastogenesis. The characteristics of these cells are quite unique because the cells possessed phagocytic function as well as macrophages but they required RANKL stimulation to survive like osteoclasts. Furthermore, we analyzed mRNA expressions during osteoclastogenesis and dendritic cell differentiation using DNA microarray technique. A transcription factor, IRF-8 was found to be downregulated during osteoclastogenesis but not in dendritic cell differentiation. The IRF-8 deficient mice exerted severe osteoporosis due to enhanced osteoclastogenesis. Moreover, over-expression of IRF-8 in osteoclast precursors strongly suppressed osteoclastogenesis but not dendritic cell differentiation. These results suggest that IRF-8 plays roles of suppression of osteoclastogenesis but not dendritic cell differentiation. These findings will contribute to establishment of new methods for the treatment of periodontal diseases and rheumatoid arthritis.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 5 results) Presentation (6 results)

  • [Journal Article] Characterization of synovial cell clones isolated from rheumatoid arthritis patients:possible involvement of TNF-α in reduction of osteoprotegerin in synovium2007

    • Author(s)
      Zhao, B., Takami, M., Miyamoto, Y., Suzawa, T., Yamada, A., Mochizuki, A., Yasuhara, R., Wang, X., Namiki, M., Sakamoto, K., Kamijo, R.
    • Journal Title

      Cytokine 41

      Pages: 61-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Heparin enhances osteoclastic bone resorption by inhibiting osteoprotegerin activity2007

    • Author(s)
      Irie, A., Takami, M., Kubo, H., Sekino-Suzuki, N., Kasahara, K., Sanai, Y.
    • Journal Title

      Bone 41

      Pages: 165-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] TGF-β suppresses POEM expression through ERK1/2 and JNK in osteoblasts2007

    • Author(s)
      Miyazono, A., Yamada, A., Morimura, N., Takami, M., Suzuki, D., Kobayashi, M., Tezuka, K., Yamamoto, M., Kamijo, R.
    • Journal Title

      FEBS Letter 581

      Pages: 5321-5326

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characterization of synovial cell clones isolated from rheumatoid arthritis patients : possible involvement of TNF-a in reduction of osteoprotegerin in synovium2007

    • Author(s)
      Zhao, B., Takami, M., Miyamoto, Y., Suzawa, T., Yamada, A., Mochizuki, A., Yasuhara, R., Wang, X., Namiki, M., Sakamoto, K., Kamijo, R
    • Journal Title

      Cytokine 41

      Pages: 61-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Heparin enhances osteoclastic bone resorption by inhibiting osteoprotegerin activity2007

    • Author(s)
      Irie, A., Takami, M., Kubo, H., Sekino-Suzuki, N., Kasahara, K., Sanai, Y
    • Journal Title

      Bone 41

      Pages: 165-174

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] TGF-β suppresses POEM expression through ERK1/2 and JNK in osteoblasts2007

    • Author(s)
      Miyazono, A., Yamada, A., Morimura, N., Takami, M., Suzuki, D., Kobayashi, M., Tezuka, K., Yamamoto, M., Kamijo, R
    • Journal Title

      FEBS Lett 681

      Pages: 5321-5326

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interleukin-4 inhibition of osteoclast differentiation is stronger than that of Interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts2007

    • Author(s)
      Yamada, A., Takami, M., Yasuhara, R., Zhao, B., Mochizuki, A., Miyamoto, Y., Eto, T., Yasuda, H., Nakamichi, Y., Kim, N., Katagiri, T., Suda, T., Kamijo, R
    • Journal Title

      Immunology 120

      Pages: 573-579

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of two biologically-crucial hydroxygroups of(-)-epigallocatechin gallate in osteoclast culture2007

    • Author(s)
      Nakagawa, H., Hasumi, K., Takami, M., Aida-Hyugaji, S., Woo, J-T., Nagai, K., Ishikawa, T., Wachi, M
    • Journal Title

      Biochem Pharmacol 73

      Pages: 34-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric Oxide in Pulp Cell Growth, Differentiation and Mineralization2007

    • Author(s)
      Yasuhara, R., Suzawa, T., Miyamoto, Y., Wang, X., Takami, M., Yamada, A., Kamijo, R
    • Journal Title

      JDent Res 86(2)

      Pages: 163-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Characterization of synovial cell clones isolated from rheumatoid arthritis patients:possible involvement ofTNF-a in reduction ofosteoprotegerin in synovium2007

    • Author(s)
      Zhao, B., Takami, M., Miyamoto, Y., Suzanwa, T., Yamada, A., Mochizuki, A., Yasuhara, R., Wang, X., Namiki, M., Sakamoto, K., Kamijo, R.
    • Journal Title

      Cytokine 41

      Pages: 61-70

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TGF-β suppresses POEM expression through ERKI/2 and JNK in osteoblasts2007

    • Author(s)
      Micazono, A., Yamada, A., Morimura, N., Takami. M., Suzuki, D., Kobayashi, M., Tezuka, K., Yamamoto, M., Kamijo, R.
    • Journal Title

      FEBS Lett. 581

      Pages: 5321-5326

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two biologically-crucial hydroxygroups of (-)-epigallocatechin gallate in osteoclast culture.2007

    • Author(s)
      Nakagawa, H.
    • Journal Title

      Biochem Pharmacol 73

      Pages: 34-43

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitric Oxide in Pulp Cell Growth, Differentiation and Mineralization.2007

    • Author(s)
      Yasuhara, R.
    • Journal Title

      J Dent Res 86・2

      Pages: 163-168

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification and Characterization of the Precursors Committed to Osteoclasts Induced by TRANCE/RANKL.2006

    • Author(s)
      Mochizuki, A.
    • Journal Title

      J Immunol 177

      Pages: 4360-4368

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differentiation of osteoclats and dendritic cells from common precursors : relationships between bone metabolism and immune system.2006

    • Author(s)
      Takami, M.
    • Journal Title

      Biolog Mechanism Tooth Eruption, Resorption, and Movement 1

      Pages: 143-149

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 破骨細胞の機能と分化 : 細胞に見る免疫と骨代謝の接点2006

    • Author(s)
      高見正道
    • Journal Title

      埼玉医科大学雑誌 33・3,4

      Pages: 80-81

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ビタミンD : 炎症・免疫2006

    • Author(s)
      高見正道
    • Journal Title

      THE BONE 20・6

      Pages: 57-61

    • Related Report
      2006 Annual Research Report
  • [Presentation] Toll-like receptors and Osteoimmunology2007

    • Author(s)
      Masamichi Takami
    • Organizer
      韓国薬学会 シンポジウム
    • Place of Presentation
      韓国 ソウル
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Toll-like receptors and Osteoimmunology2007

    • Author(s)
      Masamichi Takami
    • Organizer
      韓国薬学会シンポジゥム
    • Place of Presentation
      韓国ソウル
    • Year and Date
      2007-11-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] Symposium : "Toll-like receptors and Osteoimmunology"

    • Author(s)
      Masamichi, Takami
    • Organizer
      Pharmaceutical Society of Korea
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transcription factor IRF-8 is a negative regulator of osteoclast differentiation in vitro and in vivo

    • Author(s)
      B., Zhao, M., Takami, R., Kamijo, et. al.
    • Organizer
      29th Annual Meeting of the American Society foi Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] TGF-beta suppresses POEM expression in osteoblasts

    • Author(s)
      A., Miyazono, A., Yamada, M., Takami, et. al.
    • Organizer
      29th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Lysine-specific Gingipain(Kgp) and lipopolysaccharide(LPS) synergistically induces osteoclast differentiation

    • Author(s)
      R., Yasuhara, Y., Miyamoto, M., Takami, et. al.
    • Organizer
      29th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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