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The analysis of epigenetic regulation of osteoblastogenesis induced by BMP-2

Research Project

Project/Area Number 18592048
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

KAMIJO Ryutaro  Showa University, School of Dentistry, Professor (70233939)

Co-Investigator(Kenkyū-buntansha) KATAGIRI Takenobu  Saitama Medical University, Faculty of Medicine, Professor (80245802)
MIYAMOTO Yoichi  Showa University, School of Dentistry, Associate Professor (20295132)
TAKAMI Masamichi  Showa University, School of Dentistry, Assistant Professor (80307058)
YAMADA Atsushi  Showa University, School of Dentistry, Assistant Professor (50407558)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBMP / Histone / Methylation / Acetvlation / Osteoblastoeenesis / DNAメチル化 / ヒストンの翻訳後修飾 / 脱アセチル化 / 骨芽細胞 / 破骨細胞
Research Abstract

Bone morphogenetic protein-2 (BMP-2) stimulates osteoblast differentiation, but inhibits myogenetic differentiation in C2C12 myoblasts. To evaluate important genes for osteoblast differentiation induced by BMP-2, we performed a microarray analysis on C2C12 cells treated with or without BMP-2 for 4 days. We found that gremlin expression was lower in C2C12 cells treated with BMP-2 as compared to untreated cells. gremlin, also called downregulated by v-mos; drm belongs to dan (differential-screening selected gene aberrative in neuroblastoma) family protein, which is known as an antagonist of BMP signaling. Down-regulation of gremlin gene expression by BMP-2 occurred in both time- and dose-dependent manners. Treatment of C2C12 cells with gremlin prevented the alkaline phosphatase activity induced BMP-2. In conclusion, BMP-2 inhibited gremlin transcription in C2C12 cells, a mechanism that probably enhances BMP action during osteoblast differentiation.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 6 results) Presentation (2 results) Book (2 results)

  • [Journal Article] TGF-β suppresses POEM expression through ERK1/2 and JNK in osteoblasts2007

    • Author(s)
      Miyazono, A.
    • Journal Title

      FEBS Lett. 581

      Pages: 5321-5326

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] TGF-β suppresses POEM expression through ERK1/2 and JNK in osteoblasts2007

    • Author(s)
      Miyazono, A., et. al.
    • Journal Title

      FEBS Lett 581

      Pages: 5321-5326

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] New Frontiers in Regenerative Medicine2007

    • Author(s)
      Zhao, B., et. al.
    • Journal Title

      Springer

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] TGF-beta suppresses POEM expression through ERK1/2 and JNK in osteoblasts2007

    • Author(s)
      Miyazono A.
    • Journal Title

      FEBS Lett. 581・27

      Pages: 5321-5326

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of synovial cell clones isolated from rheumatoid arthritis patients:Possible involvement of TNF-alpha in reduction of osteoprotegerin in synovium2007

    • Author(s)
      Zhao, B.
    • Journal Title

      Cytokine 41・1

      Pages: 61-70

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FGF23 induces expression of two isoforms of NAB2,which are corepressors of Egr-12007

    • Author(s)
      Fukuda, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 353・1

      Pages: 147-151

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide in pulp cell growth, differentiation and mineralization.2007

    • Author(s)
      Yasuhara, R.
    • Journal Title

      J. Dent. Res. 86

      Pages: 163-168

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Heparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-22006

    • Author(s)
      Zhao, B.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 23246-23253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification and Characterization of the Precursors Committed to Osteoclasts Induced by TNF-related activation-induced cytokine/Receptor activator of NF-kB ligand2006

    • Author(s)
      Mochizuki, A.
    • Journal Title

      J.Immunol. 177

      Pages: 4360-4368

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Heparin potentiates the in vivo ectopic bone Formation induced by bone morphogenetic protein-22006

    • Author(s)
      Zhao, B., et. al.
    • Journal Title

      J. Biol. Chem 281

      Pages: 23246-23253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification and Characterization of the Precursors Committed to Osteoclasts Induced by TNF-related activation-induced cytokine/Receptor activator of NF-κB ligand2006

    • Author(s)
      Mochizuki, A., et. al.
    • Journal Title

      J. Immunol 177

      Pages: 4360-4368

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification and Characterization of the Precursors Committed to Osteoclasts Induced by TNF-related activation-induced cytokine/Receptor activator of NF-□B ligand.2006

    • Author(s)
      Mochizuki, A.
    • Journal Title

      J. Immunol. 177

      Pages: 4360-4368

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Heparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-2.2006

    • Author(s)
      Zhao, B.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 23246-23253

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Purification and identification of high molecular weight bone morphogenetic protein 4 from fetal bovine serum.2006

    • Author(s)
      Kodaira, K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 345

      Pages: 1224-1231

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Establishment of primary cultures for mouse ameloblasts as a model of their lifetime.2006

    • Author(s)
      Suzuwa, T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 345

      Pages: 1247-1253

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhancement of bone formation induced by implanted octacalcium phosphate involving conversion into Ca-deficient hydroxyapatite.2006

    • Author(s)
      Suzuki, O.
    • Journal Title

      Biomaterials 27

      Pages: 2671-2681

    • Related Report
      2006 Annual Research Report
  • [Presentation] IRF-8(interferon regulatory factor 8)は破骨細胞分化を抑制する転写因子である2007

    • Author(s)
      趙 宝紅
    • Organizer
      第25回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2007-07-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] IRF-&(interferon regulatory factor 8) is trtanscription factor for suppression of osteoclast differentiation2007

    • Author(s)
      Zhao, B
    • Organizer
      The 25th The Annual Meeting of the Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Osaka, JAPAN
    • Year and Date
      2007-07-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] New Frontiers in Regenerative Medicine2007

    • Author(s)
      Zhao, B
    • Total Pages
      154
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] New Frontiers in Regenerative Medicine2006

    • Author(s)
      Zhao B.
    • Total Pages
      154
    • Publisher
      Springer
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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