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Research on the induction of cell proliferation and differentiation by hyaluronic acid for dental pulp regeneration therapy

Research Project

Project/Area Number 18592094
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKyushu Dental College

Principal Investigator

KITAMURA Chiaki  Kyushu Dental College, School of Dentistry, Associate Professor (50265005)

Co-Investigator(Kenkyū-buntansha) NISHIHARA Tatsuji  Kyushu Dental College, School of Dentistry, Professor (80192251)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsDental pulp cells / Odontoblasts / Neurons / Apoptosis / Regeneration therapy / 歯髓細胞
Research Abstract

We previously demonstrated the in vivo relationship between pulp apoptosis and pulp cell proliferation and differentiation during wound healing (J Dent Res, 2001; J Dent Res, 2003). We also demonstrated in vitro that pulp cells exposed to heat stress show apoptosis and thermotolerance, which was modified by LPS through the regulation of HSF1, HSP27, Akt, and NFkB/IkBa (J Cell Biochem, 2005, 2006). We also found that hypoxia induces cell cycle arrest and apoptosis through the regulation of p21, cyclin D2, Rb, and p53 (J Dent Res, 2006). In this research, to clarify the mechanism of pulp wound healing and establish dentin/pulp regeneration therapy, we applied gelatin hydrogels incorporating FGF-2, and found that controlled release of FGF-2 from gelatin hydrogels induced formation of dentin-like particles in dentin defect above exposed pulp (J Endod, 2007). Further, we established rat dental pulp cell line with odontoblastic properties, and found that calcification ability of this odontoblastic cells was inhibited by LPS (J Endod, 2007). Now, we are continuing research on effects of glycosaminoglycan on pulp cells, odonotblasts, and neurons, which are constituents of dental pulp tissues.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (62 results)

All 2007 2006

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (37 results) Book (11 results)

  • [Journal Article] 低温軟化型ガッタパーチャを使用した根管充填法における根尖孔外溢出の臨床的比較2007

    • Author(s)
      貞方 和明
    • Journal Title

      日本歯科保存学会雑誌 50・4

      Pages: 521-529

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects oflipopolysaccharide on newly established rat dental pulp derived cell line with odontoblastic proterties2007

    • Author(s)
      Kimiko Nomiyama
    • Journal Title

      Journal of Endodontics 33・10

      Pages: 1187-1191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Formation of dentin-like particles in dentin defects above exposed pulp by controlled release of fibroblast growth factor 2 from gelatin hydrogels2007

    • Author(s)
      Naoki Kikuchi
    • Journal Title

      Journal ofEndodontics 33・10

      Pages: 1198-1202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Clinical comparison of the periapical overfilling by obturation methods using low-temperature softened gutta-percha.2007

    • Author(s)
      Kazuaki, Sadakata
    • Journal Title

      The Japanese Journal of Conservative Dentistry(peer review) 50(4)

      Pages: 521-529

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of lipopolysaccharide on newly established rat dental pulp derived cell line with odontoblastic properties2007

    • Author(s)
      Kimiko, Nomiyama
    • Journal Title

      Jounral of Endodontics(peer review) 33(10)

      Pages: 1187-1191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Formation of dentin-like particles in dentin defects above exposed pulp by controlled release of fibroblast growth factor 2 from gelatin hydrogels2007

    • Author(s)
      Naoki, Kikuchi
    • Journal Title

      Jounral of Endodontics(peer review) 33(10)

      Pages: 1198-1202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of lipopolysaccharide on newly established rat dental pulp derived cell line with odontoblastic proterties2007

    • Author(s)
      Kimiko Nomiyama
    • Journal Title

      Journal of Endodontics 33・10

      Pages: 1187-1191

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of dentin-like particles in dentin defects above exposed pulp by controlled release of fibroblast growth factor 2 from gelatin hydrogels2007

    • Author(s)
      Naoki Kikuchi
    • Journal Title

      Journal of Endodontics 33・10

      Pages: 1198-1202

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Sequential exposure to lipopolysaccharide and heat stress on dental pulp cells2006

    • Author(s)
      Chiaki Kitamura
    • Journal Title

      Journal of Cellular Biochemistry 99・3

      Pages: 797-806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Reoxygenation improves hypoxia induced pulp cell arrest2006

    • Author(s)
      Yoshiko Uena
    • Journal Title

      Journal of Dental Research 85・9

      Pages: 824-828

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of sequential exposure to lipopolysaccharide and heat stress on dental pulp cells2006

    • Author(s)
      Chiaki, Kitamura
    • Journal Title

      Journal of Cellular Biochemistry(peer review) 99(3)

      Pages: 797-806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Reoxygenation improves hypoxia induced pulp cell arrest2006

    • Author(s)
      Yoshiko, Ueno
    • Journal Title

      Journal of Dental Research(peer review) 85(9)

      Pages: 824-828

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of sequential exposure to lipopolysaccharide and heat stress on dental pulp cells.2006

    • Author(s)
      Chiaki Kitamura
    • Journal Title

      Journal of Cellular Biochemistry 99・3

      Pages: 797-806

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reoxygenation improves hypoxia induced pulp cell arrest.2006

    • Author(s)
      Yoshiko Ueno
    • Journal Title

      Journal of Dental Research 85・9

      Pages: 824-828

    • Related Report
      2006 Annual Research Report
  • [Presentation] Establishment ofrat dental pulp cell line showing odontoblastic phenotype2007

    • Author(s)
      Kimiko Nomiyama
    • Organizer
      International Association ofDental Research
    • Place of Presentation
      New Orleans,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] グリコサミノグリカンを応用した歯髄及び歯周組織の再生医療に関する研究2007

    • Author(s)
      寺下 正道
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯内治療で改善を認めない難治性歯痛患者への口腔顔面痛治療の検討2007

    • Author(s)
      諸冨 孝彦
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯内治療で改善しない難治性歯痛患者への口腔顔面痛治療の検討2007

    • Author(s)
      諸冨 孝彦
    • Organizer
      日本歯内療法学会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 各種サイトカインが石灰化能を有する歯髄細胞(KN-3)に与える影響2007

    • Author(s)
      永吉 雅人
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      埼玉
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 石灰化能を有するラット歯髄細胞におよぼすリポ多糖の影響2007

    • Author(s)
      野口 扶美子
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      岡山
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] PC12細泡の神経突起伸長におけるピアルロン酸の影響2007

    • Author(s)
      鷲尾 絢子
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      岡山
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulp Apoptosis and Regeneration Induced by External Factors. In:Resin restoratives and dentin regeneration-Partnership or hostility2007

    • Author(s)
      Chiaki Kitamura
    • Organizer
      国際歯科研究学会日本支部会(JADR)
    • Place of Presentation
      鶴見
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of rat dental pulp cell line showing odontoblastic phenotype2007

    • Author(s)
      Kimiko, Nomiyama
    • Organizer
      Conference of International Association of of Dental Research
    • Place of Presentation
      New Orleans, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on regeneration therapies of dental pulp and periodontal tissues using glycosaminoglycans2007

    • Author(s)
      Masamichi, Terashita
    • Organizer
      Conference of Kyushu Dental Society
    • Place of Presentation
      Kitakyushu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Studies of pain clinics on orofacial pain of patients whose teeth pain were not released by endodontic therapy2007

    • Author(s)
      Takahiko, Morotomi
    • Organizer
      Conference of Kyushu Dental Society
    • Place of Presentation
      Kitakyushu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Approach by pain clinics on orofacial pain of patients whose teeth pain were not released by endodontic therapy2007

    • Author(s)
      Takahiko, Morotomi
    • Organizer
      Conference of Japan Endodontic Association
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of several cytokines on dental pulp cell line(KN-3)showing mineralization ability2007

    • Author(s)
      Masato, Nagayoshi
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Saitama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of lypopolysaccharide on rat dental pulp cell line with mineralization ability2007

    • Author(s)
      Fumiko, Noguchi
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Okayama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of hyaluronic acid on neurite outgrowth of PC-12 cells2007

    • Author(s)
      Ayako, Washio
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Okayama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulp apoptosis and regeneration induced by external factors. In : Resin restoratives and dentin regeneration-Partnership or hostility?2007

    • Author(s)
      Chiaki, Kitamura
    • Organizer
      Conference of Japanese Association of Dental Research
    • Place of Presentation
      Tsurumi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] グリコサミノグリカンを応用した歯髄及び歯周組織の再生医療に関する研究.2007

    • Author(s)
      寺下 正道
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Related Report
      2007 Annual Research Report
  • [Presentation] 歯内治療で改善を認めない難治性歯痛患者への口腔顔面痛治療の検討.2007

    • Author(s)
      諸冨 孝彦
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Related Report
      2007 Annual Research Report
  • [Presentation] 歯内治療で改善しない難治性歯痛患者への口腔顔面痛治療の検討.2007

    • Author(s)
      諸冨 孝彦
    • Organizer
      日本歯内療法学会
    • Place of Presentation
      広島
    • Related Report
      2007 Annual Research Report
  • [Presentation] 各種サイトカインが石灰化能を有する歯髄細胞(KN-3)に与える影響.2007

    • Author(s)
      永吉 雅人
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      埼玉
    • Related Report
      2007 Annual Research Report
  • [Presentation] 石灰化能を有するラット歯髄細胞におよぼすリポ多糖の影響.2007

    • Author(s)
      野口 扶美子
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      岡山
    • Related Report
      2007 Annual Research Report
  • [Presentation] PC12細胞の神経突起伸長におけるヒアルロン酸の影響.2007

    • Author(s)
      鷲尾 絢子
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      岡山
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pulp Apoptosis and Regeneration Induced by External Factors. In:Res in restoratives and dentin regeneration-Partnership or hostility?-2007

    • Author(s)
      Chiaki Kitamura
    • Organizer
      国際歯科研究学会日本支部会(JADR)
    • Place of Presentation
      鶴見
    • Related Report
      2007 Annual Research Report
  • [Presentation] FGF-2徐放性ゼラチンハイドロゲル粒子が歯髄創傷治癒に与える影響2006

    • Author(s)
      菊池 直樹
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 石灰化能を有するラット由来歯髄細胞株の樹立2006

    • Author(s)
      野見山 貴美子
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] FGF-2徐放性ゼラチン粒子を用いた歯髄再生療法の検討2006

    • Author(s)
      菊池 直樹
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯髄再生療法を目的としたFGF-2徐放性ゼラチンハイドロゲル粒子の応用2006

    • Author(s)
      菊池 直樹
    • Organizer
      日本組織工学会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 石灰化能を有するラット歯髄細胞株の樹立2006

    • Author(s)
      野見山 貴美子
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      鹿児島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯髄細胞への各種グリコサミノグリカンの影響2006

    • Author(s)
      北村 知昭
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      鹿児島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯根尖周囲組織の状態が垂直加圧根管充填時の溢出に与える影響2006

    • Author(s)
      貞方 和明
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      鹿児島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of controlled release of FGF-2 from gelatin hydrogels on dental pulp wound healing2006

    • Author(s)
      Naoki, Kikuchi
    • Organizer
      Conference of Kyushu Dental Society
    • Place of Presentation
      Kitakyushu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of rat derived dental pulp cells with odontoblastic properties2006

    • Author(s)
      Kimiko, Nomiyama
    • Organizer
      Conference of Kyushu Dental Society
    • Place of Presentation
      Kitakyushu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of controlled release of FGF-2 on pulp regeneration therapy2006

    • Author(s)
      Naoki, Kikuchi
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of gelatin hydrogels showing controlled release of FGF-2 on dental pulp regeneration therapy2006

    • Author(s)
      Naoki, Kikuchi
    • Organizer
      Conference of the Japanese Society of Tissue Engineering
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of rat dental pulp cell line showing mineralization ability2006

    • Author(s)
      Kimiko, Nomiyama
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Kagoshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of several glycosaminoglycans on dental pulp cells2006

    • Author(s)
      Chiaki, Kitamura
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Kagoshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of periapical condition on overfilling of gutta-percha by vertical root canal filling method2006

    • Author(s)
      Kazuaki, Sadakata
    • Organizer
      Conference of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Kagoshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 保存修復学 第5版2007

    • Author(s)
      寺下 正道
    • Total Pages
      412
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 歯内治療学 第3版2007

    • Author(s)
      北村 知昭
    • Total Pages
      315
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Conservative Dentistry, 5th Edition2007

    • Author(s)
      Masamichi, Terashita
    • Total Pages
      412
    • Publisher
      Ishiyaku Shuppan Co., Ltd
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Endodontics, 3rd Edition2007

    • Author(s)
      Chiaki, Kitamura
    • Total Pages
      315
    • Publisher
      Ishiyaku Shuppan Co., Ltd
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 保存修復学第5版2007

    • Author(s)
      寺下 正道
    • Total Pages
      412
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2007 Annual Research Report
  • [Book] 歯内治療学第3版2007

    • Author(s)
      北村 知昭
    • Total Pages
      315
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2007 Annual Research Report
  • [Book] 保存修復学 21 第三版2006

    • Author(s)
      寺下 正道
    • Total Pages
      386
    • Publisher
      永末書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 必修臨床研修歯科医ハンドブック2006

    • Author(s)
      北村 知昭
    • Total Pages
      361
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Conservative Dentistry 21, 3rd Edition2006

    • Author(s)
      Masamichi, Terashita
    • Total Pages
      386
    • Publisher
      Nagasue Shoten
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Handbool for Intern Dentist2006

    • Author(s)
      Chiaki, Kitamura
    • Total Pages
      361
    • Publisher
      Ishiyaku Shuppan Co., Ltd
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 保存修復学21 第三版2006

    • Author(s)
      北村 知昭
    • Total Pages
      386
    • Publisher
      永末書店
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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