• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The analysis for role of enamel and enamel related protein in, during body formation

Research Project

Project/Area Number 18592099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionShowa University

Principal Investigator

YAMADA Yoshishige  Showa University, School of Dentistry, Lecturer (40360127)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Yukio  Meikai University, School of Dentistty, Professor (40207931)
KIMURA Yuichi  Ohu University, School of Dentistry, Professor (60211877)
MASADA Yoshiko  Showa University, School of Dentistry, Lecturer (10297038)
KAWANAKA Takeo  Showa University, School of Dentistry, Lecturer (10365702)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,540,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsamelogenin / gene expression / odontoblasts / in situ PCR mothod / in situ LAMP method / KLK4 / エクソン / In situ PCR法 / ラット上顎切歯 / 組織学的観察 / LAMP法 / 歯胚
Research Abstract

The expression of amelogenin in rats upper molar were studied by using in situ PCR method that detected amplified gene in histology to examine a role of amelogenin for tissue formation. Results of this study, the expression of amelogenin were confirmed in the ameloblasts and initial undifferentiational odontoblasts. Furthermore, the faintly amelogenin gene expression were observed not only in the undifferentiational but also in some degree of maturational odontoblasts. However, a lot of problems of tissue sections such as removing sections from a slide grasses and a destruction of the tissue were happened in this method because of repeating the temperatures change from high temperatures to a low temperatures quickly, Therefore a new gene amplified method was selected in stead of PCR. Recently, the LAMP method that was used four primers on gene amplification. The character of this methods are used a constant temperature of 75℃, and the amplification efficiency of this method is from ten to hundreds of times higher than a general PCR methods.
In this study, in situ LAMP method that was detected amplified gene expression in the tissue section, same as in situ PCR method was tried to detect the gene expression of amelogenin in odontoblasts and pulp cells. Previously, some researchers were reported that using in situ LAMP method to soft tissue. However there were no reports for using hard tissues. Therefore the in situ LAMP system for hard tissues has not been established now, and this study was enforced to establish a system for hard tissues with in situ LAMP method.
Moreover, one of main enamel proteases, KLK4 was also examined in this study. From the results of this study, KLK4 gene expression and protein localization were observed not only in ameloblasts, but also in oral cancer cells. This result indicated that amelogenin might has a several role for several areas of cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv2008

    • Author(s)
      Yamada Y, Hossain M, Shimizu Y, Kimura Y, Masuda Y, Nakamura Y, Matsmoto K
    • Journal Title

      Journal of Dentistry 34

      Pages: 130-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisoly2008

    • Author(s)
      Yamada Y, Hossain M, Shimizu Y, Kimura Y, Masuda Y, Nakamura Y, Matsumoto K
    • Journal Title

      Journal of Dentistry 34

      Pages: 130-137

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial plaque removal with Carisolv system:a clinical approach2007

    • Author(s)
      Yamada Y, Hossain M, Shimizu Y, Kimura Y, Masuda Y, Nakamura Y, Matsmoto K
    • Journal Title

      The journal of Clinical pediatric Dentistry 31

      Pages: 199-201

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Artificial plaque removal with Carisolv system : a clinical approach2007

    • Author(s)
      Yamada, Y, Hossain, M, Shimizu, Y, Kimura, Y, Masuda, Y, Nakamura, Y, Matsmoto, K
    • Journal Title

      The journal of Clinical pediatric Dentistry 31

      Pages: 199-201

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv2007

    • Author(s)
      Yamada, Y, Hossain, M, Shimizu, Y, Kimura, Y, Masuda, Y, Nakamura, Y, Matsmoto, K
    • Journal Title

      Journal of Dentistry 34

      Pages: 130-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Artificial plaque removal with Carisoly system: a clinical approach2007

    • Author(s)
      Yamaaa Y, Hossain M, Kimura Y, Nakamura Y, Masuda Y, Shimizu Y, Matsumoto K
    • Journal Title

      The Journal of Clinical Pediatric Dentistry 31

      Pages: 199-201

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Basic study of morphological changes and surface roughness of cavities prepared by TEA CO2 laser irradiation.2006

    • Author(s)
      Nakamura Y, Hossain M, Yamada Y, Masuda YM, Jayawardena JA, Matsumoto K
    • Journal Title

      Photomedicine and laser surgery 24・4

      Pages: 203-507

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An assessment following root canal preparation by Er, Cr : YSGG laser irradiation in straight and curved roots, in vitro2006

    • Author(s)
      Jahan KM, Hossain M, Nakamura Y, Yamada Y, Kinoshita J, Matsumoto K
    • Journal Title

      Laser Medicine and Science 21

      Pages: 229-234

    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi