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Characterization of human dental pulp-derived cell lines

Research Project

Project/Area Number 18592102
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNihon University

Principal Investigator

SUGURO Hisashi  Nihon University, 歯学部, 助教 (90318452)

Co-Investigator(Kenkyū-buntansha) 浅野 正岳  日本大学, 歯学部, 講師 (10231896)
Co-Investigator(Renkei-kenkyūsha) ASANO Masatake  日本大学, 歯学部, 専任講師 (10231896)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥660,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsヒト歯髄由来細胞株 / SV40 Large T antigen / Differentially expressed genes / Thy-1 / differential display / Toll-like receptor / TLR3 / dsRNA / 歯髄細胞 / Vaccinia virus / Transfection
Research Abstract

歯髄細胞は数種類の異なる細胞から構成されていることが知られている。 しかしながら, その活性は低く, 長期にわたる継代培養は困難である。そこで本研究では, 歯髄細胞の性質をより詳細に検索するためにヒト歯髄由来細胞の樹立と解析を行った。 本申請期間では, 9種類のクローン細胞を樹立し, 樹立したクローン細胞間の遺伝子発現の違いをDifferential Displayにより検索し, 8種類の遺伝子を検出した

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (6 results)

  • [Journal Article] Characterization of human dental pulp-derived cell lines2008

    • Author(s)
      Suguro H, Asano M, Kaneko Y, OmagariD, Ogiso B, Moro I, Komiyama K
    • Journal Title

      Int Endod J 41

      Pages: 609-616

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Distribution of muse polymeric immunoglobulin receptor (mpIgR) : Establishment of enzyme-linked immunosorbent assay system for mpIgR2008

    • Author(s)
      Omagari D, Iijima M, SuguroH , Sato I, AsanoM , Moro I
    • Journal Title

      Scand J Immunol 68

      Pages: 543-551

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト歯髄由来細胞株の樹立と解析2008

    • Author(s)
      勝呂尚, 浅野正岳, 尾曲大輔, 尾形英大,小木曽文内,茂呂周, 小宮山一雄
    • Journal Title

      消化器と免疫 44

      Pages: 24-28

    • NAID

      10019830766

    • Related Report
      2008 Final Research Report
  • [Journal Article] ヒト歯髄由来細胞株の樹立と解析2008

    • Author(s)
      勝呂 尚
    • Journal Title

      消化器と免疫 44

      Pages: 24-28

    • NAID

      10019830766

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Characterization of human dental pulp-derived cell lines2008

    • Author(s)
      勝呂 尚
    • Journal Title

      International Endodontic Journal 41

      Pages: 609-616

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential Distribution of muse polymeric immunoglobulin receptor (mpIgR) : Establishment of enzyme-linked immunosorbent assay system for mpIgR2008

    • Author(s)
      勝呂 尚
    • Journal Title

      Scandinavian Journal of Immunology 68

      Pages: 543-551

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト歯髄由来細胞株の樹立と解析2008

    • Author(s)
      勝呂尚
    • Journal Title

      消化器と免疫 (印刷中)

    • NAID

      10019830766

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Characterization of human dental pulp-derived cell lines2008

    • Author(s)
      勝呂尚
    • Journal Title

      INTERNATIONAL ENDODONTIC JOURNAL (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 根管形態および加圧法が加熱軟化根管充填の填塞性に及ぼす影響2008

    • Author(s)
      勝呂 尚
    • Organizer
      日本歯内療法学会
    • Place of Presentation
      東京歯科大学
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト歯髄由来細胞株の樹立と解析2007

    • Author(s)
      勝呂尚
    • Organizer
      '07秋季日本歯科保存学会
    • Place of Presentation
      岡山
    • Year and Date
      2007-11-08
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト歯髄由来細胞株の樹立と解析2007

    • Author(s)
      勝呂尚
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2007-11-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヒト歯髄由来細胞株の樹立と解析2007

    • Author(s)
      勝呂尚
    • Organizer
      第44回日本消化器免疫学会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-09
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト歯髄由来細胞株の樹立と解析2007

    • Author(s)
      勝呂尚
    • Organizer
      日本消化器免疫学会
    • Place of Presentation
      品川プリンスホテル
    • Year and Date
      2007-07-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] 培養ヒト培養歯髄細胞株への一時的なタンパク質発現2006

    • Organizer
      '06秋季日本歯科保存学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2006-11-09
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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