Evaluation of liver damage by direct pulp capping and development of functional materials for pulp capping with biocompatibility and tissue degeneration

• Project/Area Number: 18592105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Junsei Junior College
• Principal Investigator: NAKAMURA Mariko Junsei Junior College, Dental Hygiene Program, Professor (90284067)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Yasuhiro Okayama University, Department of Biomaterials, Graduate School of medical, Dental, and Pharmaceutical Sciences, Associate Professor (90281162) ; SUZUKI Kazuomi Okayama University, Department of Biomaterials, Graduate School of medical, Dental, and Pharmaceutical Sciences, Professor (30050058) ; TAKAHASHI Toshichika Junsei Junior College, Dental Hygiene Program, Professor (60135952) ; ISHIKAWA Kunio Kyushu University, Department of Biomaterials, Faculty of Dental Science, Professor (90202952)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: polysaccharide / photo-crosslinkable gelatin / direct pulp capping / 天然多糖類 / ゼラチン誘導体 / 天然多糖 / カルシウム塩 / Fibrinogen / Thrombin

Research Abstract

The photo-crosslinkable gelatin and phosphopullulan was employed as direct pulp capping method in dental field. Cavities with the exposed pulp area were prepared of first molars in rats. The cavity was formed using a 1/4 round bar and the pulp was eroded. The prepared cavity was washed reciprocally with 10% sodium hypochlorite and 3% hydrogen peroxide. All cavities were divided into three groups. The exposed pulps were covered with photo-crosslinkable gelatin preparation, phosphopullulan preparation and covered with conventional calcium hydroxide preparation. Then the cavities covered with photo-crosslinkable gelatin preparation were photo-irradiated. The margins of the treated areas of the molars were sealed with a protect liner. The animals were sacrificed at 1 weeks and 2 weeks after operation. In three groups, the capped treated teeth were observed at 1 and 2 weeks after operation. By 1 week after cavity preparation, the coalescence of inflammatory cells was observed subjacent to the exposed site of the dental pulp in the calcium hydroxide preparation, photo-crosslinkable gelatin preparation and phosphopullulan preparation -treated teeth. We found less inflammatory response when the cavities were treated with photo-crosslinkable gelatin preparation and phosphopullulan preparation. By 2 week after cavity preparation, the calcium hydroxide preparation and phosphopullulan preparation promote calcification. The effect of photo-curable gelatin and phosphopullulan was investigated for direct pulp capping.

Research Products

• [Journal Article] Effect of 4-MET-and 10-MDP-based primers on resin bonding to titanium. (2006)
  • Author(s): Tsuchimoto,Y, Yoshida,Y., Mine,A., Nakamura,M., Nishiyama,N., Van Meerbeek,B., Suzuki,K.
  • Journal Title: Dental Materials Journal 25 Pages: 120-124
  • NAID: 110004498265
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of 4-MET-and 10-MDP-based primers on resin bonding to titanium (2006)
  • Author(s): Tsuchimoto, Y., Yoshida, Y., Mine, A., Nakamura, M., Nishiyama, N., Van Meerbeek, B., Suzuki, K.
  • Journal Title: Dental Materials Journal 25 Pages: 120-124
  • NAID: 110004498265
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 生体硬組織接着用キット (2010)
  • Inventor(s): 吉田靖弘、田中雅人、鈴木一臣、尾崎敏文、高畑智宏、入江正郎、中村真理子、河島光伸、野尻大和、岡田浩一、長尾昌浩
  • Industrial Property Rights Holder: 国立大学法人岡山大学、クラレメディカル株式会社、学校法人高梁学園
  • Industrial Property Number: 2010-036350
  • Filing Date: 2010-02-22
  • Description: 「研究成果報告書概要(和文)」より