| Project/Area Number |
18592125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Okayama University |
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Principal Investigator |
NAWACHI Kumiko Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (10379787)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOKI Takuo Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (00225195)
KANYAMA Manabu Okayama University, University Hospital of Medicine and Dentistry, Senior Assistant Professor (90294420)
藤澤 拓生 岡山大学, 大学院・医歯薬学総合研究科, 助手 (20325096)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | BMP-2 / gelatin hydrogels / bone regeneration / implant / periodontal disease / carrier / large animal / Escherichia coli |
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| Research Abstract |
A genetically modified, recombinant human bone morphogenetic proteirr2 with two additional repeats of the heparin-binding domain, derived from E coli (rhBMP-2 T4) has shown a stronger osteoinductivity than wild-type rhBMP-2. The aim of this study was to find a suitable carrier fix rhBMP-2 T4 in order to enhance bone regeneration. The in vitro and in vivo release profiles of^<125>I-labelled rhBMP-2 T4 incorporated into 6 kinds of gelatin hydrogels with different isoelectric point (IEP) were investigated. As a result, the higher the IEP was, the longer the rhBMP2 T4 radioactivity was preserved. To elucidate the osteoinductivity difference of the rhBMP2 T4/hydrogels composites, two high IEP gelatin hydrogels: a basic gelatin hydrogel (PI9) and a modified PI9 with spermine (SM), were implanted at critical-sized defects in the rat calvaria. The mean normalized radio-opacity against the surrounding intact bone in the PI9 group was significantly higher than those in the SM (p<0.001) and the control (without carrier) groups (p<0.001). The observed radio-opacity increase in the PI9 group was clearly related to the bone formation at the defect. Therefore, we concluded that PI9 is a suitable carrier for the rhBMP-2 T4 to enhance bone regeneration.
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