| Project/Area Number |
18592136
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
補綴理工系歯学
|
|---|
| Research Institution | Health Sciences University of Hokkaido |
|---|
Principal Investigator |
YOKOYAMA Yuichi Health Sciences University of Hokkaido, SCHOOL OF DENTISTRY, ASSISTANT PROFESSOR (50295903)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAI Toshihiro HEALTH SCIENCES UNIVERSITYB OF HOKKAIDO, SCHOOL OF DENTISTRY, PROFESSOR (80014273)
KOSHINO Hisashi HEALTH SCIENCES UNIVERSITYB OF HOKKAIDO, SCHOOL OF DENTISTRY, ASSOCIATE PROFESSOR (90186669)
TANAKA Maki HEALTH SCIENCES UNIVERSITYB OF HOKKAIDO, SCHOOL OF DENTISTRY, ASSISTANT (40207139)
TOYOSHITA Yoshifumi HEALTH SCIENCES UNIVERSITYB OF HOKKAIDO, SCHOOL OF DENTISTRY, ASSISTANT (20399900)
IWASAKI Kazuo HEALTH SCIENCES UNIVERSITYB OF HOKKAIDO, SCHOOL OF DENTISTRY, ASSISTANT (90453274)
牧浦 哲司 北海道医療大学, 歯学部, 助手 (90347774)
田中 慎介 北海道医療大学, 歯学部, 助手 (10405681)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Masticatory function / Dietary alteration / Locomotor Activity / Emotional stress / Rat / ラット / 咬合 / 尿 / 血液検査 |
|---|
| Research Abstract |
Objectives :
Masticatory function is reported to be deteriorated by aging and/or tooth loss. The decrease of masticatory function is also reported to cause disorders of dietary intake and/or daily activity. We hypothesized that the dietary alteration should act as an emotional stress, and affects the hippocampal neurotransmitters resulting in locomotive activities in rat.
Methods :
To measure the locomotive activities, 5 rats fed with the solid diet and 5 rats fed with the powder diet were used by using an animal movement analysis system (Scanet MV-20 ; MELQUEST, Japan). This system consists of a square cage (565×565 mm) equipped with 144 pairs of photosensors located at 6mm intervals, horizontally and vertically. The intersection of paired photosensors in the enclosure was counted as one unit of locomotive activities. The data obtained from a 12 : 12 hour light/dark cycle were used for analysis.
Results :
As the results, the locomotive activities of the powder diet group were significantly greater than that of the solid diet group. There were significant differences in rearing score of rats between the solid diet group and the powder diet group (p < 0.05; Man-Whitney U test). As to average of difference of the locomotive activities and the rearing score in dark period, the powder diet group was significantly greater than that of the solid diet group (p < 0.05; Man-Whitney U test). In the previous study, it is reported that the measurement of locomotive activities can evaluate the activity and emotionality. Moreover, the powder diet in rats is speculated to be stressful because rats have the habit of feeding on a solid diet.
Conclusion :
From this study, it was suggested that the stress caused by the alteration of diet influenced on higher brain function in rats.
|
