| Project/Area Number |
18592172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
NAGATA Masaki Niigata University, Institute of Medicine and Dentistry, Assistant professor (10242439)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATA Koh Niigata University, Medical & Dental Hospital, Professor (80207802)
星名 秀行 新潟大学, 医歯学総合病院, 講師 (30173587)
OGOSE Akira Niigata University, Institute of Medicine and Dentistay, Associate professor (80323963)
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Sciences, Professor (50211371)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,930,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Periosteal cell / Culture / FGF2 / Bone regeneration / Implant |
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| Research Abstract |
Tb establish a effective procedure of bone regeneration compensating deformed or low alveolar ridge for dental implantology, we attempted the administration of autologous cultured periosteal cell with the bone graft. Conditoning of systemic periosteal cell culture, in tearms of a biological clean room with full-time tecnitian and committee for the salty, in 2006 was followed by clinical test of the autologous cultured periosteum in augmentation of alveolar bone.
(1) Conditoning of systemic periosteal cell culture: Construction of the biological clean room and training of full-time tecnitian for the culture; establishment of ethical and softy committees (2006).
(2) Pre-clinical test sultures: estimation of the culture procedure, serum and mediums, and other reagents were performed in the actual culture system. Growth and morphology of cells, bacterial or virus or micoplasm infections, and remnant level of the bobine serum were examined. No abnormal finding was observed in the test culture
… More
. Growth arrest was observed on sixth week after the onset of cram At the first periods of the culture, contamination of micoplasms were detected by nested-PCR analysis, but were disappeared at the end of the culture. Bovine serum remnant was under the detection limit by the immunofluorosent or dot-blot methods. No oncologic morphology, such as nodal growth or atypism, was estimated. ALP activity was maintained through the culture process, suggesting osteoblastic phenotype was maintained until the end of the culture.
(3) seven cases of autologous periosteal cell graft were experienced within the period of the project. Three of alveolar venia bone grafts, four cases of sinus floor elevations were supplemented by autologous periosteal cells. Two of the seven cases have been performed with prosthetic treatment No abnormal healing process, as well as no infection of the grafts was reported. CT image of the grafted area suggested well developed bone formation with less resorption of the graft. Less
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