• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establichment of brain oxidative model created by ferric nitrilo-triacetate(Fe-NTA)

Research Project

Project/Area Number 18592178
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

MAEDA Shigeru  Okayama University, Hospital, Department of Dental Anesthesiology, associate professor (50253000)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Toru  Okayama University, Hospital, Department of Anesthesiology, Senior Assistant Professor (40252952)
YAMAAI Yuichiro  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Oral Function and Anatomy, assistant professor (00158057)
MIYAWAKI Takuya  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Disability and Oral Sciences, and Dental Anesthesiology, Professor (00219825)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsbrain / oxidative reaction / heme oxygenase-1 / cytokine / iron / 4-hydroxy-2-nonenal / 酸化
Research Abstract

In last year, Fe-NTA was injected into peritoneal fluid. In this year, Fe-NTA was administered with intracerebroventricular (icv) injection in order to avoid effects on peripheral tissue. Fe-NTA solusion (Fe 0.1mg/ml, 0.25 μl/hr) was administered continuously into lateral ventricle using osmotic pump and brain infusion kit, and changes of the brains were evaluated. In control, normal saline solution was administered by icy injection in a same manner. After 2 weeks administration, hypothalamus, cerebral cortex, hippocampus, and striatum were dissected, and evaluated in mRNA of hemeoxygenase (HO)-1, IL-6 and superoxidedismutase (SOD) 2 with real-time PCR. Levels of HO-1 and IL-6 mRNA was raised, but SOD 2 mRNA was stable. After 4 weeks administration of Fe-NTA, brain sections were stained in HE, Pearl stain, and TUNEL. In quantitative evaluation, 4-hydroxy-2-nonenal (4-HNE) was measured by dot blot, and mRNAs of HO-1, IL-6, TNFα, SOD 1, SOD 2, SOD 3, Bc12, Bad, and FasL were analyzed by real-time PCR in the hippocampus and hypothalamus. As a result, hippocampus was damaged remarkably and replaced with inflammatory tissue in HE staining. Iron localized between inflammatory tissue and normal tissue. Apoptosis was induced in neuronal cells in same area as iron localization. Significant increases were observed in 4-HNE, HO-1, IL-6, IL-1β, and TNFα mRNA in the hippocampus and hypothalamus. In control, those changes were not observed. Given together, inflammation and oxidative changes were induced by icy injection of Fe-NTA. And since the hippocampus was damaged remarkably, it may be vulnerable to oxidative stress caused by Fe-NTA.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (6 results)

All 2008 2007 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Heme Oxygenase-1 Induction in the Brain during Lipopolysaccharide-induced Acute Inflammation2008

    • Author(s)
      Shigeru, MAEDA, Ichiro, NAKATSUKA, Yukiko, HAYASHI, Hitoshi, HIGUCHI, Masahiko, SHIMADA, Takuya, MIYAWAKI
    • Journal Title

      Neuropsychiatric Disease and Treatment (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Heme Oxygenase-1 Induction in the Brain during Lipopolysaccharide-induced Acute Inflammation2008

    • Author(s)
      Maeda S, Nakatsuka I, et. al.
    • Journal Title

      Neuropsychiatric Disease and Treatment (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heme Oxygenase-1 Induction in the Brain during Lipopolysac charide-induced Acute Inflammation

    • Author(s)
      Maeda S, Nakatsuka I, et. al.
    • Journal Title

      Neuropsychiatric Disease and Treatment (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Presentation] Brain Oxidative Reactions to Continuous Intracerebroventricular Injeciton of Ferric Nitrilotriacetate (Fe-NTA)2007

    • Author(s)
      Maeda S, Hayashi Y, Higuchi H, Miyawaki T
    • Organizer
      37th Annual Meeting of the Society for Neuroscience.
    • Place of Presentation
      米国 サンディエゴ
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Brain Oxidative Reactions to Continuous Intracerebroventricular Injeciton of Ferric Nitrilotriacetate (Fe-NTA)2007

    • Author(s)
      Maeda S, Hayashi Y, Higuchi H, Miyawaki T
    • Organizer
      37th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      米国サンディエゴ
    • Year and Date
      2007-11-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brain Oxidative Reactions to Continuous Intracerebroventricular Injeciton of Ferric Nitrilotriacetate(Fe-NTA)2007

    • Author(s)
      Maeda, S., Hayashi, Y., Higuchi, H., Miyawaki, T
    • Organizer
      37th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi