Effect of folic acid on cleft palate prevention induced with dioxins
Project/Area Number |
18592179
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Okayama University |
Principal Investigator |
YAMADA Tomohiro Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Dentistry (60335619)
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Co-Investigator(Kenkyū-buntansha) |
MISHIMA Katsuaki Okayama University, Hospital, Oral and Maxillofacial Reconstructive Surgery, Senior Assistant Professor (60304317)
SUGAHARA Toshio Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Professor (10116048)
|
Project Period (FY) |
2006 – 2007
|
Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
Keywords | dioxin / mouse / cleft palate / prevention |
Research Abstract |
In 2006, we examined preventive effect of folic acid for dioxin (TCDD) induced cleft palate in vivo. ICR strain mice of 8-10 weeks of age were used. After 2 weeks administration of folic acid. female mice were impregnated. TCDD was administered with gastric tubes at 40 μg/kg at gestation day (GD) 12, and all fetuses were examined at GD 18. However, folic acid had no preventive effects on TCDD induced cleft palate. Then, in 2007, we changed the aim of this research to investigation of the more detailed mechanisms of TCDD induced cleft palate. In the result of SEM (scanning electron microscope), HE staining, immunohistochemical staining (TGFB3, MSX1, FGFR1/2, LHX8), and organ culture examination, development of fetal palates administered TCDD delayed, but fused as same as normal palates once, however, after fusion phase they ruptured and became cleft palates.
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Report
(3 results)
Research Products
(54 results)
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[Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2,3,7,8 - Tetrachlorodibenzo - p - dioxin in mice2008
Author(s)
Fujiwara, K., Yamada, T., Mishima, K., Imura, H., Sugahara, T
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Journal Title
Congenital Anomalies 48(2)
Pages: 68-73
NAID
Description
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[Journal Article] Functional requirement of CCN2 for intramembranous bone formation in embryonic mice2008
Author(s)
Kawaki, H., Kubota, S., Suzuki, A., Yamada, T., Matsumura, T., Mandai, T., Yao, M., Maeda, T., Lyons, KM., Takigawa, M
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Journal Title
Biochem Biophys Res Commun 366(2)
Pages: 450-456
Description
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[Journal Article] Respiratory status during sleeping after orthognathic surgery with mandibular setbacks2008
Author(s)
Yamada, T., Mishima, K., Matsumura, T., Sugii, A., Imura, H., Sugahara, T
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Journal Title
Asian J Oral Maxiricrac Surg 20(in press)
Description
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[Journal Article] Effects of 2,3,7,8- tetrachlorodibenzo- p- dioxin (TCDD) on the development of the murine palate in organ culture2007
Author(s)
Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Sugahara, T
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Journal Title
Asian J Oral IfkxillofPc Surg 19(4)
Pages: 192-196
Description
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[Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells2006
Author(s)
Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Moritani, N., Sugahara, T
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Journal Title
Asian J Oral Maxillofac Surg 18
Pages: 89-94
Description
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[Journal Article] Cleft Lip and Palate in Mice Treated with 2,3,7,8- Tetrachlorodibenzo-p- dioxin : a Morphological in vivo Study2006
Author(s)
Yamada, T., Mishima, K., Fujiwara, K., Imura, H., Sugahara, T
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Journal Title
Congenital Anomalies 46(1)
Pages: 21-25
NAID
Description
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[Journal Article] Clinical study of cleft lip and palate patients at Okayama University Hosoital2006
Author(s)
Imura, H., Mishima, K., Yamada, T., Kagawa, T., Oura, A., Sugahara, T
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Journal Title
Okayama Univ Dent J 25(2)
Pages: 33-36
Description
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[Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells.2006
Author(s)
Yamada, T., Mishima, K., Fujiwara, K., Imura, H., Moritani, N., Sugahara, T.
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Journal Title
Asian J Oral Maxillofac Surg 18
Pages: 89-94
Related Report
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[Journal Article] Cleft Lip and Palate in Mice Treated with 2,3,7,8- Tetrachlorodibenzo- p- dioxin : a Morphological in vivo Study.2006
Author(s)
Yamada, T., Mishima, K., Fujiwara, K., Imura, H., Sugahara, T.
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Journal Title
Congenital Anomalies 46(1)
Pages: 21-25
NAID
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[Presentation] Safty of release of MMF in emergency, part 22007
Author(s)
Nakano, M., Sugahara, T., Honda, K., Yamada, T., Ueno, T
Organizer
The 61th Japanese Congress of Stomatology
Place of Presentation
Kobe
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[Presentation] Is MMF necessary after a rigid fixation SSRO?2007
Author(s)
Yamada, T., Mishima, K., Matsumura, T., Sugii, A., Imura, H., Sugahara, T
Organizer
The 52th Japanese Congress of Oral and Maxillofacial Surgery
Place of Presentation
Nagoya
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[Presentation] An experimental model of cleft palate exposed to TCDD : an immunohistological examination2007
Author(s)
Imura, H., Yamada, T., Mishima, K., Ueno, T., Matsumura, T., Kondo, S., Hirata, A., Fujiwara, K., Sugii, A., Sugahara, T
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The 52th Japanese Congress of Oral and Maxillofacial Surgery
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[Presentation] Mechanism of palatal fusion associated with localization of parlcan and heparanase2007
Author(s)
Hirata, A., Ueno, T., Yamachika, E., Yamada, T., Kagawa, T., Imura, H., Sugahara, T
Organizer
The 52th Japanese Congress of Oral and Maxillofacial Surgery
Place of Presentation
Naeova
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[Presentation] Is MFF necessary after a rigid fixation SSRO?2007
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Organizer
American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
Place of Presentation
Honolulu
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[Presentation] An experimental model of cleft palate exposed to TCDD : an immunohistological examination2007
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Organizer
American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
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Year and Date
2006-10-12
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