| Budget Amount *help |
¥3,030,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
The aim of this study was To do an artificial material necessary for a blood vessel bunch surroundings in vivo for reproducing the bone organization, to use the method of reproducing the bone organization with an artificial blood vessel handle, and to examine the possibility of an actual clinical application and it was researched to transplant the bone organization with the blood vessel handle actually played to the mandibular bone by using a large-scale animal among the same animals, and to examine stability of the reproducing bone.
1. The reproduction bone with the blood vessel handle which makes β-TCP a work stand is made the use of AV shunt loop of the Hiroga movements pulse.
It experimented on chamber (research representative making)which made β-TCP done as follows by making, and the Nainu beagle and 16 male use. The crowd which observed the reproduction bone: It divided into eight crowds which reproduced the jawbone by using the reproduction bone with eight. Moreover, the crowd whi
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ch observed the reproduction bone: PRP administering group of eight: 4 and non-administering group: It was assumed four. In addition, it similarly went to the PRP administering group and non-administering group by using the reproduction bone in eight crowds which reproduced the jawbone.
2. Analysis of reproduction bone
It was made to be euthanatized in 1.8 six months later and chamber was removed from the Hiroshi line of the backbone on both sides under. At this time, even the armpit movements pulse flaked off, and the Cabtocda movements pulse was removed with chamber.
2. The catheter is inserted from the cutting edge of the movements pulse at the same time as removing chamber, the angiography medicine (sulfuric acid Barriyum of the gelatin content 5%60% and Urografin) is administered, and a newborn blood vessel in the reproduction bone is confirmed by X rays of taking a picture.
3. After it took a picture of X rays of, escape ash H-E dye specimen was made, and this specimen material was retrieved in histology. As a result, β-TCP which did in each crowd admitted absorbing, and the substitution of the fiber externals cell was admitted.
5. It makes comparative study of area of a newborn blood vessel in the reproduction bone and amount (V) of the reproduction bone organization in the PRP administering group and non-administering group and the immunity histochemistry to the fiber externals cell that was substituted for the bamboo whisk in the future is retrieved. As for the presence of the bone inducement cell and the difference of the Cotskei performance in each amount, it was, and the formed reproduction bone was the separation bones of the examination with the blood vessel handle which was able to be transplanted.
3. Transplant of beagle of reproduction bone with blood vessel handle to mandibular bone
The thrombus was admitted when blood tube was combined in all samples and the causing life wear was not admitted though the reproduction bone was transplanted to mandibular bone of four beagle dogs. Less
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