Regulatory effects of methoxyphenols on the transcription factor activation in mammalian cells.

• Project/Area Number: 18592197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Meikai University
• Principal Investigator: MURAKAMI Yukio Meikai University, 歯学部, 准教授 (00286014)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,670,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 口腔外科学一般(含病態検査学) / メトキシフェノール化合物 / 転写因子 / 天然植物性フェノール / COX-2 / フェノール作用 / P. gingivalis線毛 / bi-phenol / melatonin / メトキシフェノール / 抗酸化作用 / 抗炎症作用 / 炎症性サイトカイン / P.gingivalis線毛 / o-Vanillin / curcumin / ο-Vanillin / フェノール化合物 / Ferulic Acid / Natural products / BHA / OH基解離エンタルピー

Research Abstract

We investigated the regulatory effects of methoxyphenols such as BHA, bis-BHA, o-vanillin, guaiacol, curcumin, tetrahydrocurcumin, phenol and biphenol, on the transcription factor activation stimulated by LPS or other stimulants in mammalian cells. Methoxyphenols which hardly abstracted an H atom from the phenolic OH group inhibited a transcriptional activity of redox-sensitive transcription factors, and suppressed COX-2 and cytokine expression. These observations suggested that methoxyphenol compounds possessed a therapeutic potential against chronic inflammation and mutagenesis by its phenol function.

Research Products

• [Journal Article] Antioxidant and cyclooxygenase-2-inhibiting activity of 4, 4'-biphenol, 2, 2'-biphenol and phenol. (2009)
  • Author(s): Murakami, Y., Ishii, H., Hoshina, S., Takada, N., Ueki, A., Tanaka, S., Kadoma, Y., Ito, S., Machino, M., Fujisawa, S.
  • Journal Title: Anticancer Res. 29 Pages: 2403-2410
• [Journal Article] Antioxidant and cyclooxygenase-2-inhibiting activity of 4,4'-biphenol, 2,2'-biphenol and phenol (2009)
  • Author(s): Yukio Murakami, et al
  • Journal Title: Anticancer Research 29 Pages: 2403-2410
  • Peer Reviewed
• [Journal Article] Comparative anti-inflammatory activities of curcumin and tetrahydrocurcumin based on the phenolic O-H bond disociation enthalpy, ionization potential and quantum chemical descriptor. (2008)
  • Author(s): Murakami Y., Ishii, H., Takada, N., Tanaka, S., Machino, M., Ito, S., Fujisawa, S.
  • Journal Title: Anticancer Res. 28 Pages: 699-708
• [Journal Article] Comparative anti-inflammatory activities of curcumin and tetrahydrocurcumin based on the phenolic O-H bond dissociation enthalpy, ionization potential and quantum chemical descriptor. (2008)
  • Author(s): Murakami,Y., Shoji,M., Ogiwara,T., Tanaka,S., Yokoe,I., Fujisawa,S.
  • Journal Title: Anticancer Res 28 Pages: 699-708
  • Peer Reviewed
• [Journal Article] Comparative anti-inflammatory activities of curcumin and tetrahydrocurcumin based on the phenolic O-H bond dissociation enthalpy, ionization potential and quantum chemical descriptor. (2008)
  • Author(s): Yukio Murakami, et al
  • Journal Title: Anticancer Research 28巻 Pages: 699-708
  • Peer Reviewed
• [Journal Article] Predicting the biological activities of 2-methoxyphenol antioxidants: Effects of dimers. (2007)
  • Author(s): Fujisawa, S., Ishihara, M., Murakami, Y., Atsumi, T., Kadoma, Y., Yokoe, I.
  • Journal Title: In Vivo. 21 Pages: 181-188
  • NAID: 120005520715
• [Journal Article] Re-evaluation of cyclooxygenase-2-inhibiting activity of vanillin and guaiacol in macrophages stimulated with lipopolysaccharide. (2007)
  • Author(s): Murakami, Y., Hirata, A., Ito, S., Shoji, M., Tanaka, S., Yasui, T., Machino, M., Fujisawa, S.
  • Journal Title: Anticancer Res. 27 Pages: 801-808
• [Journal Article] Preventive effect of ortho dimer of butylated hydroxyanisole on activator protein -1 activation and cyclooxygenase-2 expression in macrophages stimulated by fimbriae of Porphyromonas gingivalis (2007)
  • Author(s): Murakami,Y., Shoji,M., Ogiwara,T., Tanaka,S., Yokoe,I., Fujisawa,S.
  • Journal Title: an oral anaerobe. Anticancer Res. 27 Pages: 801-808
  • Peer Reviewed
• [Journal Article] Yokoe,I. : Predicting the biological activities of 2-methoxyphenol antioxidants : Effects of dimers. (2007)
  • Author(s): Fujisawa,S., Ishihara,M., Murakami,Y., Atsumi,T., Kadoma,Y.
  • Journal Title: In Vivo. 21 Pages: 181-188
  • Peer Reviewed
• [Journal Article] Re-evaluation of cyclooxygenase-2- inhibiting activity of vanillin and guaiacol in macrophages stimulated with lipopolysaccharide. (2007)
  • Author(s): Yukio Murakami, et. al.
  • Journal Title: Anticancer Research 27巻 Pages: 801-808
  • Peer Reviewed
• [Journal Article] Predicting the biological activities of 2-methoxyphenol antioxidants: Effects of dimers. (2007)
  • Author(s): Seiichiro Fujisawa, et. al.
  • Journal Title: In Vivo 21巻 Pages: 181-188
  • Peer Reviewed
• [Journal Article] An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophage stimulated by Porphyromonas gingivalis fimbriae. (2006)
  • Author(s): Murakami, Y., Shoji, M., Hirata, A., Tanaka, S., Hanazawa, S., Yokoe, I., Fujisawa, S.
  • Journal Title: Arch. Biochem. Biophys. 449 Pages: 171-177
• [Journal Article] Preventive effect of ortho dimer of butylated hydroxyanisole on activator protein-1 activation and cyclooxygenase-2 expression in macrophages stimulated by fimbriae of Porphyromonas gingivalis, an oral anaerobe. (2006)
  • Author(s): Murakami, Y., Shoji, M., Ogiwara, T., Tanaka, S., Yokoe, I., Fujisawa, S.
  • Journal Title: Anticancer Res. 26 Pages: 2915-2920
• [Journal Article] An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophages stimulated by Porphyromonas gingivalis fimbriae (2006)
  • Author(s): Murakami,Y., Shoji,M., Hirata,A., Tanaka,S., Hanazawa,S., Yokoe,I., Fujisawa,S
  • Journal Title: Arch. Biochem. Biophys. 449 Pages: 171-177
  • Peer Reviewed
• [Journal Article] An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophages stimulated by Porphyromonas gingivalis fimbriae. (2006)
  • Author(s): Yukio Murakami, et al.
  • Journal Title: Archives of Biochemistry and Biophysics 449巻・1-2号 Pages: 171-177
• [Journal Article] Preventive effect of ortho dimer of butylated hydroxyanisole on activator protein-1 activation and cyclooxygenase-2 expression in macrophages stimulated by fimbriae of Porphyromonas gingivalis, an oral anaerobe. (2006)
  • Author(s): Yukio Murakami, et al.
  • Journal Title: Anticancer Research 26巻・4B号 Pages: 2915-2920
• [Presentation] p-クレゾール二量体によるLPS刺激TNF-α発現の調節作用 (2009)
  • Author(s): 石井宏明, 村上幸生, 他
  • Organizer: 第62回日本口腔科学会
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2009-04-16
• [Presentation] p-クレゾール二量体によるLPS刺激TNF-α発現の調節作用 (2009)
  • Author(s): 石井宏明, 村上幸生, 保科修平, 高田直樹, 藤澤盛一郎, 町野守
  • Organizer: 第63回日本口腔科学会
  • Place of Presentation: 浜松
• [Presentation] p-クレゾール二量体によるLPS刺激TNF-α発現の調節作用 (2009)
  • Author(s): 石井宏明、村上幸生、保科修平、高田直樹、藤澤盛一郎、町野 守
  • Organizer: 第62回日本口腔科学会
  • Place of Presentation: 浜松
• [Presentation] フェノール関連二量体化合物によるLPS刺激TNF-α発現の抑制作用. (2008)
  • Author(s): 村上幸生, 他
  • Organizer: 第21回日本口腔診断学会
  • Place of Presentation: 日本大学会館
  • Year and Date: 2008-09-19
• [Presentation] フェノール関連二量体化合物によるLPS刺激TNF-α発現の抑制作用 (2008)
  • Author(s): 村上幸生, 石井宏明, 高田直樹, 丸山剛央, 湯原健司, 保科修平, 小林聡子, 細谷明代, 田中庄二, 藤澤盛一郎, 町野守
  • Organizer: 第21回日本口腔診断学会
  • Place of Presentation: 東京
• [Book] Molecular Targets and Therapeutic Uses of Spices. Modern Uses for Ancient Medicine(Chapter 5 Cloves (Eugenol)) (2009)
  • Author(s): Kadoma, Y., Murakami, Y., Atsumi, T., Ito, S., Fujisawa, S.
  • Publisher: World Scientific Publishing Co. Pre. Ltd.
• [Book] Chapter 5 Cloves(Eugenol), in Molecular Targets and Therapeutic Uses of Spices.Modern Uses for Ancient Medicine (2009)
  • Author(s): Kadoma, Y., Murakami, Y., et al
  • Total Pages: 32
  • Publisher: World Scientific Publishing Co.Pre.Ltd.
• [Book] Chapter 5. Cloves (Eugenol) in Molecular Targets and Therapeutic Uses of Spices. Modern Uses for Ancient Medicine by Editors B.(B. Aggawal and A.B. Kunnumakkara. World Scientific Publishing Co. Pre. Ltd) (2009)
  • Author(s): Kadoma,Y., Murakami,Y., Atsumi,T., Ito,S., Fujisawa,S.
  • Publisher: 5 Toch Link, Singapore 596224