ESTABLISHMENT OF TUMOR SPECIFIC MOLECULER TARGETTING THERAPY AGAINST HYPDXIA INDUCIBLE FACTOR
| Project/Area Number |
18592201
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
HATORI Masashi Showa University, Dept Oral Maxillofac Surg., Associate Professor (20245814)
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| Co-Investigator(Kenkyū-buntansha) |
TOYOSHIMA Takahiko Showa Univ., Dept Oral Maxillofac Surg., Assistant Professor (90338533)
ITO Hidetoshi Showa Univ., Dept Oral Maxillofac Surg., Assistant Professor (80384303)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | HYPOXIA / OSCC / CELL CYCLE / MMP / INVASIVENESS / 線維芽細胞 |
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| Research Abstract |
1.Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via G1 phase cell cycle wrest
1) Hypoxia did not affect cell viability of OSCC cells up to 8 hour incubation
2) Hypoxia did not affect cell proliferation of NA and SAS, whereas the proliferation ofHSC-3, CA9-22 was down-regulated.
3) Hypoxia sensitive cells (HS) display enhanced expression of HIF-1 α within 24 h incubation in hypoxia chamber.
4) Hypoxia resistant cells (HR) display constitutive expression of HIF-1α.
5) HS showed G1 arrest under the hypoxic condition.
6) Enhanced cyto-toxicity of 5-FU was observed in HS.
2, Effects of hypoxia on cell invasiveness of OSCC cells
1) Hypoxia enhances invasiveness of OSCC cells in Matrigel invasion assay.
2) Hypoxia induces expression of MMP-2, MMP-9 of OSCC cells.
3) Hypoxia ski induces secretion of MMPs in culture supernatant.
4) Activated MMP-2 in OSCC cells was up-regulated by hypoxia
5) Fibroblast conditioned medium cultured in hypoxia chamber enhanced MMP-2 expression.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Enhanced susceptibility to apoptosis of oral squamous cell carcinoma cells subjected to combined treatment with anticancer drugs and phosphatidylinositol 3-kinase inhibitors2007
Author(s)
Iwase, M, Yoshiba, S, Uchida, M, Takaoka, S, Kurihara, Y, Ito, D, Hatori, M, Shintani, S
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Journal Title
Int J Oncol 31
Pages: 1141-1147
Description
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[Journal Article] Thalidomide suppresses melanoma growth by activatiing natural killer cells in mice2006
Author(s)
Kawamata, A, Ito, D, Odani, T, Isobe, T, Iwase, M, Hatori, M, Nagumo, M
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Journal Title
Oncology Report 16
Pages: 1231-1236
Description
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[Presentation] Effects of hypoxia on cell invasiveness of OSCC cells
Author(s)
Yuji, Kuria, Masashi, Hatori, Yuriko, Kinygasa, Masako, Saito, Hidetoshi, Ito, Masaru, Ohaghi, Masao, Nagumo
Organizer
The 30th Annual meeting of Japan society for Head andNeck Cancer
Description
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