| Project/Area Number |
18592204
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
MAMIYA Hideki Tokyo Dental College, Dental Anesthesiology, Assistant Professor (30266572)
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| Co-Investigator(Kenkyū-buntansha) |
ICHINOHE Tatsuya Tokyo Dental College, Dental Anesthesiolog, Professor (40184626)
KANEKO Yuzuru Tokyo Dental College, Dental Anesthesiology, Professor (00085747)
ANZAI Kazunori National Institute of Radiological Sciences, 放射線安全研究センター, Team Leader (70128643)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Autoradiography / Radioisotope / Local Anesthetics / Drug Distribution |
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| Research Abstract |
Localization of lidocaine in oral mucosa of the rabbit was visualized using imaging plate autoradiography. Under general anesthesia, 14C-labeled 2% lidocaine solution with or without epinephrine was injected into oral mucosa or periodontal ligament of rabbits. Five minutes after injection, animals were killed and frozen. Slices of animals' head were obtained using a cryomicrotome. Bio-Imaging Analyzer System was used to visualize the accumulation of 14C in samples. To determine localization of 14C-labeled lidocaine in oral tissue, we scanned the same sliced sample and superimposed the two obtained images. Injected 14C-labeled lidocaine was clearly visualized. Localization of lidocaine with epinephrine around the root apex was more apparent than that without epinephrine. Labeled lidocaine injected into the periodontal ligament was observed along the root surface. This method thus appears to be useful for visualizing the localization of lidocaine in tissue.
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