Molecular Mechanism of invasion in Oral squamous cell carcinomas.-small GTPase regulates the invasion
| Project/Area Number |
18592212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University (2007)
Kinki University (2006) |
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Principal Investigator |
NAKAHARA Hirokazu (2007) Osaka University, Dental Hospital, Associate Professor (70324796)
大谷 朋弘 (2006) 近畿大学, 医学部附属病院, 助手 (30411622)
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| Co-Investigator(Kenkyū-buntansha) |
中原 寛和 大阪大学, 歯学部附属病院, 助教授 (70324796)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | cancer / invasion / metastasis / small GTPase / invadopodia / 口腔扁平上皮癌 / 浸潤・転移 / Rhoファミリー / Invadopodia / フィブロネクチン分解モデル / 浸潤転移 / invadopodia / フィブロネクチン分解・浸潤モデル |
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| Research Abstract |
Epithelial-mesenchymal interaction is crucial in invade surrounding tissues by metastatic tumor cells. Here, we presented modified an in vitro fibronectin degradation/invasion assay that was co-cultured tumor cell with fibroblast. Using this assay, we estimated invasive activity as to three cell lines derived from human oral squamous cell carcinomas with different invasion mode. According to Yamamoto Kohama's classification, OSC20 was classified into grade 3, OSC19 into grade 4C and HOC313 into grade 4D. HOC313 showed strong invasiveness in co-cultured with fibroblast than OSC20 and OSC19 did. Also, knock down for MT1-MMP of cancer cell or/and fibroblast completed inhibit in vitro invasiveness.
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Report
(3 results)
Research Products
(8 results)
