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Molecular Mechanism of invasion in Oral squamous cell carcinomas.-small GTPase regulates the invasion

Research Project

Project/Area Number 18592212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOsaka University (2007)
Kinki University (2006)

Principal Investigator

NAKAHARA Hirokazu (2007)  Osaka University, Dental Hospital, Associate Professor (70324796)

大谷 朋弘 (2006)  近畿大学, 医学部附属病院, 助手 (30411622)

Co-Investigator(Kenkyū-buntansha) 中原 寛和  大阪大学, 歯学部附属病院, 助教授 (70324796)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordscancer / invasion / metastasis / small GTPase / invadopodia / 口腔扁平上皮癌 / 浸潤・転移 / Rhoファミリー / Invadopodia / フィブロネクチン分解モデル / 浸潤転移 / invadopodia / フィブロネクチン分解・浸潤モデル
Research Abstract

Epithelial-mesenchymal interaction is crucial in invade surrounding tissues by metastatic tumor cells. Here, we presented modified an in vitro fibronectin degradation/invasion assay that was co-cultured tumor cell with fibroblast. Using this assay, we estimated invasive activity as to three cell lines derived from human oral squamous cell carcinomas with different invasion mode. According to Yamamoto Kohama's classification, OSC20 was classified into grade 3, OSC19 into grade 4C and HOC313 into grade 4D. HOC313 showed strong invasiveness in co-cultured with fibroblast than OSC20 and OSC19 did. Also, knock down for MT1-MMP of cancer cell or/and fibroblast completed inhibit in vitro invasiveness.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (1 results)

  • [Journal Article] Inhibition of Rac induces Hyper-Activation of c-Jun N-Terminal Kinase and Caspase-Dependent Apoptosis.2008

    • Author(s)
      Matsuoka Y., Nakahara H., Otani T., et. al.
    • Journal Title

      Oral Science International 5.1(In press)

    • NAID

      10024185946

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 線維芽細胞との混合培養条件下での口腔扁平上皮癌細胞の基底膜浸潤制御-MT1-MMPの役割-2008

    • Author(s)
      松岡裕大, 中原寛和, 久島潔大谷朋弘, 他
    • Journal Title

      日口口腔外科学会誌 54

      Pages: 44-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 口腔扁平上皮癌細胞の低分子GTP結合タンパク質Rac抑制によるアポトーシス誘導2008

    • Author(s)
      松岡裕大, 中原寛和, 他
    • Journal Title

      日本口腔組織培養学会誌 17

      Pages: 27-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of Rac Induces Hyper-Activation of c-Jun N-Terminal Kinase and ,Caspase-Dependent Apoptosis2008

    • Author(s)
      Matsuoka, Y, Nakahara, H, Otani, T, Kogo, M
    • Journal Title

      Oral Science International 5(in press)

    • NAID

      10024185946

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mechanism of apoptosis by inhibition of Rac, small G proteins2008

    • Author(s)
      Matsuoka, Y, Nakahara, H, et. al.
    • Journal Title

      Jap. J. Tissue Cult. Dent. Res 15

      Pages: 49-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Molocular mechanism of invasiveness of oral squamous cell carcinoma co-cultured with fibroblasts-A fiction role for metalloproteinase MTI-MMP-2008

    • Author(s)
      Matsuoka, Y, Nakahara, H, et. al.
    • Journal Title

      Jap. J. Oral Maxillofacial. Surg 54

      Pages: 44-49

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 線維芽細胞との混合培養条件下での口腔扁平上皮癌細胞の浸潤制御2008

    • Author(s)
      松岡裕大、中原寛和、他
    • Journal Title

      日本口腔外科学会雑誌 54

      Pages: 44-49

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Involvemnet of Moand WD40 repeat protein in epithelial-mesenchymal Transition of oral squamous cell carcinoma cells.2007

    • Author(s)
      Nakahara, H., et. al.
    • Organizer
      第65回日本癌学会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-10-04
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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