| Project/Area Number |
18592214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | University of Toyama |
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Principal Investigator |
WADA Shigehito University of Toyama, University Hospital, lecturer (50303219)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Ryohei University of Toyama, Radiological Sciences, Lecturer (60334736)
KONDO Takashi University of Toyama, Radiologiaal Sciences, Professor (40143937)
TABUCHI Yoshiaki University of Toyama, Life Science Research Center, Asso. Professor (20322109)
古田 勲 富山大学, 大学院医学薬学研究部, 教授 (10014268)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Hyperthermia / Radiation / Oxidative stress / Oral cancer / Reactive oxygen / 活性酸素増幅法 / 細胞内酸化ストレス |
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| Research Abstract |
The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays containing approximately 16,600 genes and computational gene expression analysis tools. When the cells were treated for 10 min at 44 degrees C (15% apoptosis level), 39 up-regulated and 3 down-regulated genes were identified. In the up-regulated genes, apoptosis-and unfolded protein response-associated genes were contained. The combined treatment with heat and either chemical enhanced apoptosis level (approximately 30%) and showed a chemical-specific gene expression pattern. Furthermore, the expression levels of selected genes were confirmed by a real-time quantitative PCR. The present results will provide a basis for further understanding the molecular mechanisms in enhancement of heat-induced apoptosis by different intracellular oxidative stress.
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