Roles of MAPKs in molecular response to mechanical stimulation in chondrocytes

• Project/Area Number: 18592228
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Ichiro Tohoku University, 大学院・歯学研究科, 准教授 (70241643)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,040,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 軟骨細胞分化 / 機械的刺激応答 / 細胞内情報伝達 / ERK-1 / 2 / 細胞接着 / MAPK阻害剤 / 圧縮力 / 牽引力

Research Abstract

本研究では、機械的伸展刺激負荷にともない、ERKのリン酸化が起こること、さらに、ERK-1/2の活性化阻害剤を培養系に添加することにより、分化中の軟骨細胞のメカニカルストレスレスポンスを阻害できることをII型コラーゲンの遺伝子発現の半定量的解析により解明した。これにより、ERK-1/2を介した細胞内情報伝達系が軟骨細胞の機械的刺激受容機構において細胞外からの刺激を核内に伝達している可能性が示唆された。

Research Products

• [Journal Article] Molecular mechanisms of mechanical stress response during chondrogenesis (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
  • Journal Title: J Biomech Sci Eng (in press)
  • NAID: 130000129002
  • Peer Reviewed
• [Journal Article] The differentiation of chondrocytes under cyclic mechanical strain by unique culture system (2007)
  • Author(s): Masuda T, Takahashi I, Anada T, Arai F, Fukuda T, Takano-Yamamoto T, Suzuki O
  • Journal Title: J Biotechol 13 Pages: 231-238
  • Peer Reviewed
• [Journal Article] Mechanobiological signal transduction in differentiating chondrocyte and new configuration for mechanical stress culture (2007)
  • Author(s): Takahashi I, Masuda T, Arai F, Anada T, Fukuda T, Suzuki O, Takano-Yamamoto T
  • Journal Title: Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pages: 1061-1064
  • Peer Reviewed
• [Journal Article] Mechanical stretch inhibits chondrogenesis through ERK1/2 phosphorylation in micromass culture (2007)
  • Author(s): Takahashi I, Terao F, Masuda T, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Journal Title: Interface Oral Health Science 2007 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Mechanical stretch inhibits chondrogenesis through ERKI/2 phosphorylation in micromass culture. (2007)
  • Author(s): Takahashi I, Masuda T, et. al.
  • Journal Title: Interface Oral Health Science 2007 Pages: 160-166
  • Peer Reviewed
• [Journal Article] The differentiation of chondrocytes under cyclic mechanical strain by unique culture system. (2007)
  • Author(s): Masuda T, Takahashi I, et. al.
  • Journal Title: J Biotechol 13 Pages: 231-238
  • Peer Reviewed
• [Journal Article] Expression of genes for gelatinases and tissue inhibitors of metalloproteinases in periodontal tissues during orthodontic tooth movement. (2006)
  • Author(s): Takahashi I et al.
  • Journal Title: J Mol Histol 37, 8-9 Pages: 333-342
• [Presentation] Molecular Mechanisms of Response to Mechanical Stimulation in Chondrocyte Differentiation (2009)
  • Author(s): Takahashi I.
  • Organizer: Symposium for Interface Oral Health Science
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-15
• [Presentation] Molecular mechanisms of response to mechanical stimulation in chondrocyte differentiation (2009)
  • Author(s): Takahashi I
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] 軟骨細胞における機械的刺激応答の分子機構について (2008)
  • Author(s): 高橋一郎, 益田泰介, 高坂久美子, 寺尾文恵, 笹野泰之, 鈴木治, 山本照子
  • Organizer: 第5回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-19
• [Presentation] 機械的伸展刺激はERK1/2シグナルの活性化を介して軟骨細胞の分化を抑制する (2008)
  • Author(s): 高橋一郎, 寺尾文恵, 高坂久美子, 益田泰介, 笹野泰之, 鈴木治, 山本照子
  • Organizer: 第29回東北骨代謝研究会
  • Place of Presentation: 仙台
  • Year and Date: 2008-02-02
• [Presentation] 硬組織形成細胞の分化とメカニカルストレス (2008)
  • Author(s): 鈴木治, 高橋一郎, 益田泰輔, 山本照子, 新井史人
  • Organizer: 第31回日本バイオレオロジー学会
  • Place of Presentation: 東京
• [Presentation] 軟骨細胞の機械的刺激応答機構におけるMAPKが果たす役割 (2007)
  • Author(s): 高橋一郎, 益田泰輔, 寺尾文恵, 笹野泰之, 鈴木治, 山本照子
  • Organizer: 第4回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-06-04
• [Presentation] 軟骨細胞の機械的刺激応答機構におけるMAPKが果たす役割 (2007)
  • Author(s): 高橋一郎, 益田泰輔, 寺尾文恵, 山本照子, 他
  • Organizer: 第4回 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台国際センター(仙台)
  • Year and Date: 2007-06-04
• [Presentation] Development of mechanical strain cell culture system for mechanobiological analysis (2007)
  • Author(s): Masuda T, Takahashi I, Matsui A, Arai F, Takano-Yamamoto T, Suzuki O
  • Organizer: 第2回International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
• [Presentation] Mechanobiological signal transduction in differentiating chondrocyte and new configuration for mechanical stress culture (2007)
  • Author(s): Takahashi I, Masuda T, Arai F, Anada T, Fukuda T, Suzuki O, Takano-Yamamoto Y
  • Organizer: IEEE-NEMS
  • Place of Presentation: Bangkok, Thailand