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Mechanism of neuropathic pain in maxillofacial region and its association with inflammatory cytokines

Research Project

Project/Area Number 18592235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionHiroshima University

Principal Investigator

TANAKA Eiji  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor (40273693)

Co-Investigator(Kenkyū-buntansha) TANNE Kazuo  Hiroshima University, Graduate School of Biomedical Sciences, Professor (30159032)
WATANABE Mineo  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor (80325183)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsNeuropathic pain / Cytokine / Pain transmission / Behavior test / Infraorbital nerve
Research Abstract

The current literature strongly implicates a role for proinflammatory cytokines such as interleukin (IL)-1 β and tumor necrosis factor-α in the genesis or maintenance of hyperalgesia. The cytokine cascade has emerged as an important contributor to the development of persistent pain states. Importantly, the involvement of cytokines in persistent pain does not appear to be limited to peripheral sensitization. Independent of release of cytokines from the periphery site of injury, inflammatory cytokines are induced in the central nerve system (CNS). IL-1 β is a prototype proinflammatory cytokine that is highly inducible in the CNS after injury. By acting on IL-1 receptors (IL-1R) that are present on neurons and glia, IL-1 β can activate distinct intracellular signaling pathways and influence cellular function. It has been shown that IL-1 β interacts with N-methyl-D-aspartate (NMDA) receptors through activation of Src family protein tyrosine kinases, leading to enhanced NMDA receptor function. The present study directly addresses the role of proinflammatory cytokines in orofacial pain mechanisms following the chronic constriction injury (CCI) of the infraorbital nerve (IN). We demonstrate that IL-1 beta is selectively activated in astroglia in the spinal trigeminal nucleus including the subnuclei interpolaris/caudalis (Vi/Vc) transition zone and caudal subnucleus caudalis (Vc), two important regions for processing of orofacial noxious input.
In conclusion, CCI of IN induced a upregulation of IL-1 beta in the spinal trigeminal nucleus including the laminated Vc and Vi/Vc. Mechanical hyperalgesia and allodynia at orofacial region developed after the chronic constriction injury of infraorbital nerve. The upregulated IL-1 beta was selectively colocalized with GFAP, a marker for astrocytes. Intrathecal infusion of IL-1ra, the naturally occurring antagonist of IL-1R, prevented the development of hyperalgesia/allodynia in orofacial region.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (12 results)

All 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (4 results)

  • [Journal Article] Glial-Cytokine-Neuronal Interactions Underlying the Mechanisms of Persistent Pain2007

    • Author(s)
      Watanabe M, Guo W, Wang H, et. al.
    • Journal Title

      Journal of Neuroscience 27・22

      Pages: 6006-6018

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Glial-Cytokine-Neuronal Interactions Underlying the Mechanisms of Persistent Pain2007

    • Author(s)
      Watanabe, M., Guo, W., Wang, H., Shimizu, K., Zou, S., LaGraize, SC., Wei, F., Dubner, R., Ren, K
    • Journal Title

      Journal of Neuroscience 27(22)

      Pages: 6006-6018

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Solid diet mastication reduces c-Fos expression in the rat trigeminal subnucleus caudalis following orofacial inflammation induced by CEA.2006

    • Author(s)
      Nishi M, Iwabe T, Tanaka E, et. al.
    • Journal Title

      Biomedical Research India 17・1

      Pages: 33-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Serotonergic neurons in the dorsal raphe nucleus are not activated by MK-801 during tooth movement.2006

    • Author(s)
      Shibazaki T, Hattori Y, Tanaka E, et. al.
    • Journal Title

      Biomedical Research India 17・2

      Pages: 99-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Solid diet mastication reduces c-Fos expression in the rat trigeminal subnucleus caudalis following orofacial inflammation induced by CFA2006

    • Author(s)
      Nishi, M., Iwabe, T., Tanaka, E., Hattori-Myoishi, Y., Kawai, N., Suekawa, Takahashi, K., Watanabe, M., Uchida, T., Tanne, K
    • Journal Title

      Biomedical Research India 17(1)

      Pages: 33-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Serotonergic neurons in the dorsal raphe nucleus are not activated by MK-801 during tooth movement2006

    • Author(s)
      Shibazaki, T., Hattori, Y., Tanaka, E., Watanabe, M., Nishi, M., Tanne, K., Yoshida, N
    • Journal Title

      Biomedical Research India 17(2)

      Pages: 99-103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Solid diet mastication reduces c-Fos expression in the rat trigeminal subnucleus caudalis following orofacial inflammation induced by CFA.2006

    • Author(s)
      Nishi M, Iwabe T, Tanaka E, et al.
    • Journal Title

      Biomedical Research India 17・1

      Pages: 33-38

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Serotonergic neurons in the dorsal raphe nucleus are not activated by MK-801 during tooth movement.2006

    • Author(s)
      Shibazaki T, Hattori Y, Tanaka E, et al.
    • Journal Title

      Biomedical Research India 17・2

      Pages: 99-103

    • Related Report
      2006 Annual Research Report
  • [Presentation] An experimental model of spared nerve injury in the orofacial region2007

    • Author(s)
      Takahashi, K., Watanabe, M., Suekawa, Y., Myoishi, Y., Nishi, M., Tanaka, E., Tanne, K
    • Organizer
      65th Meeting of Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] IL-1 β in the trigeminal nucleus is associated with allodynia/hyperalgesia following chronic constriction infraorbital nerve injury2007

    • Author(s)
      Suekawa, Y., Watanabe, M., Takahashi, K., Myoishi, Y., Nishi, M., Tanaka, E., Tanne, K
    • Organizer
      65th Meeting of Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A spared trigeminal nerve injury induces allodynia/hyperalgesia on facial regions2007

    • Author(s)
      Takahashi, K., Watanabe, M., Suekawa, Y., Nishi, M., Tanaka, E., Uchida, T., Tanne, K
    • Organizer
      49th Meeting of Japanese Society of Oral Sciences
    • Place of Presentation
      Sapporo
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A spared trigeminal nerve injury induces allodynia/hyperalgesia on facial regions2007

    • Author(s)
      高橋拡史、渡邉峰朗、末川洋平、他
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌・北海道
    • Year and Date
      2007-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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