Analysis of periodontal ligament cells derived from permanent teeth and primary teeth on tooth root resorption.

• Project/Area Number: 18592239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: HASEGAWA Tomokazu Kyushu University, University Hospital, Assistant Professor (50274668)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: root resorption / osteoclastogenesis / RANKL / amelogenin / M-CSF / fibronectin / Fibronectin / Osteoclastogenesis / Amelogenin / M-CS / 乳歯 / 遺伝子

Research Abstract

Although root resorption of deciduous teeth occurs physiologically in periods of permanent tooth eruption, abnormal root resorption frequently occurs when bacterial infection in the root canal caused inflammation around the root apex. Moreover, root resorption of a deciduous tooth sometimes occurs spontaneously when the succeeding permanent tooth is lacking. Therefore, it is possible that periodontal ligament (PDL) cells can play a role in root resorption by regulating osteoclastogenesis.
This study investigated the effect of amelogenin on osteoclastogenesis. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis. Futhermore, amelogenin inhibited the expression of receptor activator of nuclear factor kappaB ligand (RANKL) ,macrophage-colony stimulating factor (M-CSF) and fibronectin in osteoblasts, while RANKL expression was induced by fibronectin and inhibited by treatment with fibronectin small interfering RNA. These results suggest that the inhibitory effect of amelogenin on osteoclastogenesis lead to downregulation of RANKL, M-CSF and fibronectin production in osteoblasts.
Therefore, these data suggest that PDL cells which contain the epithelial rests of Malassez may inhibit root resorption.

Research Products

• [Journal Article] Amelogenin is a negative regulator of osteoclastogenesis via down-regulation of RANKL, M-CSF and fibronectin expression in osteoblasts (2007)
  • Author(s): Nishiguchi, M., Yuasa, K., Saito, K., Fukumoto, E., Yamada, A., Hasegawa, T., Yoshizaki, K., Kamasaki, Y., Nonaka, K., Fujiwara, T. and Fukumoto, S.
  • Journal Title: Archives of Oral Biology 52 Pages: 237-243
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Amelogenin is a negative regulator of osteoclastogenesis via down-regulation of RANKL, M-CSF and fibronectin expression in osteoblasts (2007)
  • Author(s): Nishiguchi.,M., Yuasa.,K., Saito,K., Fukumoto,E., Yamada,A., Hasegawa,T., Yoshizaki,K., Kamasaki,Y., Nonaka,K., Fujiwara, T.,Fukumoto,S
  • Journal Title: Arch Oral Biol 52(3) Pages: 237-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Amelogenin is a negative regulator of osteoclastogenesis via down-regulation of RANKL,M-CSF and fibronectin expression in osteoblasts. (2007)
  • Author(s): Nishiguchi., M., Yuasa, K., Saito, K., Fukumoto, E., Yamada, A., Hasegawa T., Yoshizaki, Kamasaki, Y., Nonaka, K., Fujiwara, T., Fukumoto, S.
  • Journal Title: Archives of Oral Biology 52 Pages: 237-243
  • Peer Reviewed
• [Journal Article] Current topics in pharmacological research on bone metabolism:osteoclast differentiation regulated by glycosphingolipids (2006)
  • Author(s): Fukumoto, S., Iwamoto, T., Sakai, E., Yuasa, K., Fukumoto, E., Yamada, A., Hasegawa. T., Nonaka, K. and Kato, Y.
  • Journal Title: J Phannacol Sci 100 Pages: 195-200
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Current topics in pharmacological research on bone metabolism : , osteoclast differentiation regulated by glycosphingolipids. Review (2006)
  • Author(s): Fukumoto,S.,Iwamoto, T., Sakai, E., Yuasa,K., Fukumoto,E., Yamada,A., Hasegawa,T., Nonaka,K., KatoY
  • Journal Title: J Pharmacol Sci 100(3) Pages: 195-200
  • Description: 「研究成果報告書概要(欧文)」より