An anti-c-Fms Antibody Inhibits Orthodontic Tooth Movement

• Project/Area Number: 18592242
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KITAURA Hideki Nagasaki University, Graduate School of Biomedical Sciences Course of Medical and Dental Sciences Department of Translational Medicine Division of Orthodontics and Dentofacial Orthopedics, Ph.D. Instructor (60295087)
• Co-Investigator(Kenkyū-buntansha): YOSHIMATSU Masako Nagasaki University, Graduate School of Biomedical Sciences Course of Medical and Dental Sciences Department of Translational Medicine Division of Orthodontics and Dentofacial Orthopedics, Ph.D. Instructor (20420630)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Osteoclast / TNF-α / Orthodontic

Research Abstract

Orthodontic force induces osteoclastogenesis in vivo. It has recently been reported that administration of an antibody against the macrophage-colony-stimulating factor(M-CSF)receptor c-Fms blocks osteoclastogenesis and bone erosion induced by tumor necrosis factor-α(TNF-α)administration. This study aimed to examine the effect of an anti-c-Fms antibody on mechanical loading-induced osteoclastogenesis and osteolysis in an orthodontic tooth movement model in mice. Using TNF receptor 1 and 2-deficient mice, we showed that orthodontic tooth movement was mediated by TNF-α. We injected anti-c-Fms antibody daily into a local site, for 12 days, during mechanical loading. The anti-c-Fms antibody significantly inhibited orthodontic tooth movement, markedly reduced the number of osteoclasts in vivo and inhibited TNF-α-induced osteoclastogenesis in vitro. These findings suggest that M-CSF plays an important role in mechanical loading-induced osteoclastogenesis and bone resorption during orthodontic tooth movement mediated by TNF-α.

Research Products

• [Journal Article] An anti-c-Fms Antibody Inhibits Orthodontic Tooth Movement (2008)
  • Author(s): Kitaura H., Yoshimatsu M., Fujimura, Y., Eguchi, T., Kotiara H., Yoshida, N.
  • Journal Title: J.Dent, Res. 87 Pages: 396-400
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] An anti-c-Fms antibody inhibits orthodontic tooth movement (2008)
  • Author(s): Kitaura, H., Yoshimatsu, M., Fujimura, Y., Nakao, N., Eguchi, T., Yoshida, N
  • Journal Title: J. Dent. Res. 87(4) Pages: 396-400
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Treatment of a patient with metal hypersensitivity after ort hodontic surgery (2007)
  • Author(s): Kitaura H., Fujimura, Y., Nakao, N., Eguchi, T., Yoshida, N.
  • Journal Title: Angle Orthod 77 Pages: 2544-2551
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Syk, c-Src, the αvβ3 integrin and ITAM immwloreceptors,in concert,regulate osteoclastic bone resorption (2007)
  • Author(s): Zou, W., Kitaura, H., Reeve J., Long, F., Tybulewicz, VLJ., Shattil, SJ., Ginsberg MH., Ross, FP., Teitelbaum SL.
  • Journal Title: J.Cell Biol. 176 Pages: 877-888
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Noninvasive imaging of osteoclasts in parathyroid hormone-in duced osteolysis using a 64Cu-laUeled RGD peptide (2007)
  • Author(s): Sprague, JE., Kitaura, H., Zou, W., Ye, Y., Achilefu, S., Weibaecher, KN., Teitelbaim, SL., Anderson, CJ.
  • Journal Title: J.Nucl.Med. 48 Pages: 311-318
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency (2007)
  • Author(s): Maruyama, Z., Yoshida, CA., Furuichi, T., Amizuka, N., Ito, M., Fukuyama, R., Miyazaki, T., Kitaura, H., Nakamura, K., Fujita, T., Kanatani, N., Moriishi, T., Yamana, K., Liu, W., Kawaguchi, H., Nakamura, K., Komori
  • Journal Title: Dev Dyn. 236 Pages: 1876-1890
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Dexamethsone suppresses bone formation via the osteoclast (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Bnewer, JA., Muglia LJ., Ross, FP., Teitelbaum SL.
  • Journal Title: Adv.Exp.Med.Biol. 602 Pages: 43-46
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Glucocorticoids and the osteoclast (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Brewer, JA., Muglia LJ., Ross, FP., Teitelbaum SL.
  • Journal Title: Ann N Y Acad Sci. 1116 Pages: 335-339
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Treatment of a patient with metal hypersensitivity after orthodontic surgery (2007)
  • Author(s): Kitaura, H., Fujimura, Y., Nakao, N., Eguchi, T., Yoshida, N
  • Journal Title: Angle Orthod 77(5) Pages: 923-930
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Syk, c-Src, the avb3 integrin and ITAM immunoreceptors, in concert, regulate osteoclastic bone resorption (2007)
  • Author(s): Zou, W., Kitaura, H., Reeve, J., Long, F., Tybulewicz, VLJ., Shattil, SJ., Ginsberg, MH., Ross, FP., Teitelbaum, SL
  • Journal Title: J. Cell Biol. 176(6) Pages: 877-888
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Noninvasive imaging of osteoclasts in parathyroid hormone-induced osteolysis using a 64Cu-labeled RGD peptide. (2007)
  • Author(s): Sprague, JE., Kitaura, H., Zou, W., Ye, Y., Achilefu, S., Weibaecher, KN., Teitelbaim, SL., Anderson, CJ
  • Journal Title: J. Nucl. Med 48 Pages: 311-318
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency (2007)
  • Author(s): Maruyama, Z., Yoshida, CA., Furuichi, T., Amizuka, N., Ito, M., Fukuyama, R., Miyazaki, T., Kitaura, H., Nakamura, K., Fujita, K., Kanatani, N., Moriishi, T., Yamana, K., Liu, W., Kawaguchi, H., Nakamura, K., Komori, T
  • Journal Title: Dev Dyn 236(7) Pages: 1876-1890
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dexamethsone suppresses bone formation via the osteoclast (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Brewer, JA., Muglia, LJ., Ross, FP., Teitelbaum, SL
  • Journal Title: Adv. Exp. Med. Biol. 602 Pages: 43-46
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Glucocorticoids and the osteoclast. (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Brewer, JA., Muglia, LJ., Ross, FP., Teitelbaum, SL
  • Journal Title: Ann N Y Acad Sci. 1116 Pages: 335-339
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Treatment of a patient with metal hypersensitivity after orthodontic surgery. (2007)
  • Author(s): Fujimura, Y., Nakao, N., Eguchi, T., Yoshida, N.
  • Journal Title: Angle Orthod 77 Pages: 2544-2551
  • Peer Reviewed
• [Journal Article] syk, c-Src,the αvβ3 integrin and ITAM immunoreceptors,in concert,regulate osteoclastic bone resorption (2007)
  • Author(s): Zou, W., Kitaura, H., Reeve J., Long, F., Tybulewicz, VLJ., Shattil, SJ., Ginsberg MH., Ross, FP., Teitelbaum SL
  • Journal Title: J.Cell Biol 176 Pages: 877-888
  • Peer Reviewed
• [Journal Article] Noninvasive imaging of osteoclasts in parathyroid hormone-induced osteolysis using a 64Cu-labeled RGD peptide (2007)
  • Author(s): Sprague, JE., Kitaura, H., Zou, W., Ye, Y., Achilefu, S., Weibaecher, KN., Teitelbaim, SL., Anderson, CJ
  • Journal Title: J.Nucl.Med 48 Pages: 311-318
  • Peer Reviewed
• [Journal Article] Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency (2007)
  • Author(s): Maruyama, Z., Yoshida, CA., Furuichi, T., Amizuka, N., Ito, M., Fukuyama, R., Miyazaki, T., KiLaura, H., Nakamura, K., Fujita, T., Kanatarsi, N., Moriishi, T., Yamana, K., Liu, W., Kawaguchi, H., Nakamura, K., Komori
  • Journal Title: Dev Dyn 236 Pages: 1876-1890
  • Peer Reviewed
• [Journal Article] Dexamethsone suppresses bone formation via the osteoclast (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Bnewer, JA., Muglia LJ., Ross, FP., Teitelbaum SL
  • Journal Title: Adv.Exb.Med.Biol 602 Pages: 43-46
  • Peer Reviewed
• [Journal Article] Giucocorticoids and the osteoclast (2007)
  • Author(s): Kim, HJ., Zhao, H., Kitaura, H., Bhattacharyya, S., Bnewer, JA., Muglia LJ., Ross, FP., Teitelbaum SL
  • Journal Title: Ann N Y Acad Sci 1116 Pages: 335-339
  • Peer Reviewed
• [Journal Article] The hemoglobin receptor protein of Porphyromonas gingivalis inhibits receptor activator NF-kB ligand-induced osteoclastogenesis from bone marrow macrophages. (2006)
  • Author(s): Fujimura, Y., et al.
  • Journal Title: Infect.Immun. 74(5) Pages: 2544-2551
• [Journal Article] IL-18 induces apoptosis of adherent bone marrow cells in TNF-α mediated osteoclast formation in synergy with IL-12. (2006)
  • Author(s): Kitaura, H., et al.
  • Journal Title: Immunol.Lett. 107(1) Pages: 22-31
• [Journal Article] Glucocorticoids suppress bone formation via the osteoclast. (2006)
  • Author(s): Kim, HJ., et al.
  • Journal Title: J.Clin.Invest. 116(8) Pages: 2152-2160
• [Journal Article] SHIP1 negatively regulates proliferation of osteoclast precursors via Akt-dependent alterations in D-type cyclins and p27. (2006)
  • Author(s): Zhou, P., et al.
  • Journal Title: J.Immunol 177(12) Pages: 8777-8784
• [Presentation] M-CSF Receptor C-fms Antibody Inhibits Mechanical Stress-lnduced Root.Resorption (2007)
  • Author(s): Kitaura H, Fujimura Y, Yoshimat.su M, Eguchi T, KoharaH, Yoshida N.
  • Organizer: The 29th Annual Meeting of the American So ciety for Bone and Mineral Research
  • Place of Presentation: Hawaii,U.S.A.
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T細胞のTNF-αによる破骨細胞形成でのin vivoでのかかわりあいについての検討 (2007)
  • Author(s): 北浦 英樹、吉田 教明
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] M-CSF Receptor C-fms Antibody Inhibits Mechanical Stress-Induced Root Resorption (2007)
  • Author(s): Kitaura, H., Fujimura, Y., Yoshimatsu, M., Eguchi, T., Kohara, H., Yoshida, N
  • Organizer: The 29th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Hawaii, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Marrow stromal cells modulate the contributions of macrophages and T-cells to TNF-alpha induced osteoclastogenesis (2007)
  • Author(s): Kitaura, H., Yoshida, N
  • Organizer: The 49th Annual Meeting of Japanese Asoociation for Oral Biology
  • Place of Presentation: Sapporo Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] M-CSF Receptor C-fms Antibody Inhibits Mechanical Stress-Indused Root Resorhtion (2007)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Eguchi T, Kohara H, Yoshida N
  • Organizer: The 29th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Hawaii, U.S.A.
• [Presentation] T細胞のTNF-αによる破骨紹胞形成でのin vivoでのかかわりあいについての検討 (2007)
  • Author(s): 北浦 英樹, 吉田 教明
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.mh.nagasaki-u.ac.jp/ortho_hp/porion/achievements/index.html
• [Remarks]
  • URL: http://www.mh.nagasaki-u.ac.jp/ortho_hp/porion/achievements/index.html