Resolution of quorum-sensing systems in dental plaque biofilm and development of the method that prevents the plaque formation by the control of them
| Project/Area Number |
18592243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HOSHINO Tomonori Nagasaki University, Hospital of Medicine and Dentistry, Instructor (00359960)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Taku Nagasaki University, Graduate School of Biomedical Sciences, Professor (00228975)
KAN Saito Nagasaki University, 医歯部・歯学総合附属病院, Assistant Professor (40380852)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | mutans group streptococci / Quorumsensing / real time RT-PCR / glucan biofilm / glucosyltransferase / secretory IgA / two-dimensional SDS-PAGE / オートインデューサー / ミティスグループレンサ球菌 / コンピテントスティミュレーティングペプチド / ペプチド核酸 |
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| Research Abstract |
Dental plaque is the biofilm which is formed on the tooth surface, and is a protective barrier for the bacteria against the change of the environment, the antibiotic, and the host immune response. First this biofilm is formed by the extracellular polysaccharide(glucan), which the mitis group streptococci produce from sugar, and then is reinforced by the adhesive and water-insoluble glucan, which the mutans group streptococci produce. Dental plaque biofilm plays an important role in the caries formation. In this biofilm, the plaque bacteria communicate with other bacteria by the auto-inducer like hormone. By this communication system that is commonly known as quorum-sensing, the bacterial population density in the dental plaque is thought to be kept constant, and the bacterial mass against the host immune system is constructed.
In this study, we analyzed gtfB, gtfC, gtfD, dexA, and b-galactosidase genes, using real-time RT-PCR based on the comparative CT method and demonstrated that three gtf and β-galactosidase genes were suppressed; however, dexA was upregulated. Thus, S. mutans could control biofilm mass by balancing glucan production and hydrolysis. Such control is necessary to maintain colonization on a smooth surface and to ensure that external carbohydrate present across the glucan layer is not used. On the other hand, the epitope assay of glycosyltransferase was performed and the anti-glycosyltransferase DNA vaccines were developed. Moreover, the change of the secretory IgA Secretion and the bacterial numbers in saliva by menstruation cycle were analyzed.
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Report
(3 results)
Research Products
(9 results)
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[Presentation] Expression Analysis of Streptococcus mutans dexA, gtfB, gtfC, gtfD, and p-galactosidase gene in Glucan Biofilm
Author(s)
Sato, K., Hoshino, T., Saito, K., Fujiwara, T
Organizer
The 49th Annual Meeting of Japanese Association for Oral Biology
Place of Presentation
Hokkaido University(Sapporo)
Description
「研究成果報告書概要(欧文)」より
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