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Resolution of quorum-sensing systems in dental plaque biofilm and development of the method that prevents the plaque formation by the control of them

Research Project

Project/Area Number 18592243
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionNagasaki University

Principal Investigator

HOSHINO Tomonori  Nagasaki University, Hospital of Medicine and Dentistry, Instructor (00359960)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Taku  Nagasaki University, Graduate School of Biomedical Sciences, Professor (00228975)
KAN Saito  Nagasaki University, 医歯部・歯学総合附属病院, Assistant Professor (40380852)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsmutans group streptococci / Quorumsensing / real time RT-PCR / glucan biofilm / glucosyltransferase / secretory IgA / two-dimensional SDS-PAGE / オートインデューサー / ミティスグループレンサ球菌 / コンピテントスティミュレーティングペプチド / ペプチド核酸
Research Abstract

Dental plaque is the biofilm which is formed on the tooth surface, and is a protective barrier for the bacteria against the change of the environment, the antibiotic, and the host immune response. First this biofilm is formed by the extracellular polysaccharide(glucan), which the mitis group streptococci produce from sugar, and then is reinforced by the adhesive and water-insoluble glucan, which the mutans group streptococci produce. Dental plaque biofilm plays an important role in the caries formation. In this biofilm, the plaque bacteria communicate with other bacteria by the auto-inducer like hormone. By this communication system that is commonly known as quorum-sensing, the bacterial population density in the dental plaque is thought to be kept constant, and the bacterial mass against the host immune system is constructed.
In this study, we analyzed gtfB, gtfC, gtfD, dexA, and b-galactosidase genes, using real-time RT-PCR based on the comparative CT method and demonstrated that three gtf and β-galactosidase genes were suppressed; however, dexA was upregulated. Thus, S. mutans could control biofilm mass by balancing glucan production and hydrolysis. Such control is necessary to maintain colonization on a smooth surface and to ensure that external carbohydrate present across the glucan layer is not used. On the other hand, the epitope assay of glycosyltransferase was performed and the anti-glycosyltransferase DNA vaccines were developed. Moreover, the change of the secretory IgA Secretion and the bacterial numbers in saliva by menstruation cycle were analyzed.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (9 results)

All 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] Amelogenin binds to both heparan sulfate and bone morphogenetic protein 2 and pharmacologically suppresses the effect of noggin2008

    • Author(s)
      Saito, K., Konishi, I., Nishiguchi, M., Hoshino, T., Fujiwara, T
    • Journal Title

      Bone (in press)

    • NAID

      120006981723

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Amelogenin binds to both heparan sulfate and bone morphogenetic protein 2 and pharmacologically suppresses the effect of noggin

    • Author(s)
      K. Saito, I. Konishi, M. Nishiguchi, T. Hoshino, T. Fujiwara
    • Journal Title

      Bone (In press)

    • NAID

      120006981723

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Presentation] アデノウイルスベクターを用いた抗グルコシルトランフェラーゼDNAワクチンの構築と抗体誘導能2007

    • Author(s)
      星野 倫範、斎藤 幹、藤原 卓
    • Organizer
      第49回歯科基礎医学会学術大会並びに総会
    • Place of Presentation
      北海道大学(札幌)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 生理周期による唾液中s-IgAの分泌量と口腔内細菌数の変化について2007

    • Author(s)
      星野 佑子、鮎瀬 卓郎、星野 倫範、藤原 卓
    • Organizer
      第49回歯科基礎医学会学術大会並びに総会
    • Place of Presentation
      北海道大学(札幌)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] グルカンバイオフィルム中のStrep tococcus mutansのdexA,gtfB,gtfC,gtfD,β-galactosidase遺伝子の発現について2007

    • Author(s)
      星野 佑子、鮎瀬 卓郎、星野 倫範、藤原 卓
    • Organizer
      第49回歯科基礎医学会学術大会並びに総会
    • Place of Presentation
      北海道大学(札幌)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] グルカンバイオフィルム中のStreptococcus mutansの dexA、gtfB、gtfD、β-galactosidase遺伝子の発現について2007

    • Author(s)
      ○佐藤 恭子, 星野 倫範, 齋藤 幹, 藤原 卓
    • Organizer
      第49回歯科基礎医学会学術大会並びに総会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of Anti-Glucosyltransferase DNA Vaccines using AdenoviruVector and Their Inducibility of Antibody

    • Author(s)
      Hoshino, T., Saito, K., Fujiwara, T
    • Organizer
      The 49th Annual Meeting of Japanese Association for Oral Biology
    • Place of Presentation
      Hokkaido University(Sapporo)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Change of the Secretory IgA Secretion and the Bacterial Numbers in Saliva by Menstruation Cycle

    • Author(s)
      Hoshino, Y., Ayuse, T., Hoshino, T., Fujiwara, T
    • Organizer
      The 49th Annual Meeting of Japanese Association for Oral Bioldgy
    • Place of Presentation
      Hokkaido University(Sapporo)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression Analysis of Streptococcus mutans dexA, gtfB, gtfC, gtfD, and p-galactosidase gene in Glucan Biofilm

    • Author(s)
      Sato, K., Hoshino, T., Saito, K., Fujiwara, T
    • Organizer
      The 49th Annual Meeting of Japanese Association for Oral Biology
    • Place of Presentation
      Hokkaido University(Sapporo)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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