Function of BMP2 in the development of mouse mandible by organ culture and si RNA

• Project/Area Number: 18592255
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: FUKUI Tadayoshi Tsurumi University, 歯学部, 助教 (10267544)
• Co-Investigator(Kenkyū-buntansha): YAMANE Akira 鶴見大学, 歯学部, 准教授 (20166763)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,060,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: BMP2 / RNA干渉法 / TIMP1 / 舌筋 / 器官培養法 / マウス / メッケル軟骨 / MMP13 / 舌 / 器官培養

Research Abstract

骨形成因子(BMP)は様々な組織・器官の発生・発達に重要な役割を果たしている増殖因子である。この研究ではマウス胎生期下顎および舌形成にBMPの果たす役割について調べた。その結果BMP2が金属プロテアーゼ13(MMP13)とMMP阻害因子1(TIMP1)の発現バランスを調節することによりマウス胎生期の舌形成を制御していることが明らかになった。

Research Products

• [Journal Article] ビスホスホネートの薬理 (2008)
  • Author(s): 山根 明
  • Journal Title: 歯界展望 111 Pages: 1094-1097
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement ofteeth (2007)
  • Author(s): LUAN, X., ITO, Y., IIOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T. M., FUKUI, T. YAMANE, A., BEGOLE, E., EVANS, C., and DIEKWISCII, T. G. II
  • Journal Title: J. Histochem. Cytochem. 55(2) Pages: 127-140
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue (2007)
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M. and YAMANE, A.
  • Journal Title: Cell Tissue Res. 329(1) Pages: 103-117
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse (2007)
  • Author(s): TOMOIIIRO, T. , YAMANE, A. and ASADA, Y.
  • Journal Title: Archs. Oral Biol 52(9) Pages: 828-835
• [Journal Article] Expressions of BMP2, BMP4, and their receptors during the development of mouse tongue muscle (2007)
  • Author(s): SUGA, T., FUKUI, T., MORITO, M. and YAMANE, A.
  • Journal Title: Jpn. J. Gerodontol 22(3) Pages: 280-287
  • NAID: 10025742851
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X, ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T. M., FUKUI, T. YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH, T. G. H.
  • Journal Title: J. Histochem. Cytochem. 55(2):, 2007 55 Pages: 127-140
  • Peer Reviewed
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscletissues of mouse embryonic tongue (2007)
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T. C. H., MORITO, M., YAMANE, A
  • Journal Title: Cell Tissue Res. 329 Pages: 103-117
  • Peer Reviewed
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse 07 (2007)
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. 52(9): 828-835, 20 52 Pages: 828-835
  • Peer Reviewed
• [Journal Article] Expressions of BMP2, BMP4, and their receptors during the development of mouse tongue muscle (2007)
  • Author(s): SUGA, T., FUKUI, T., MORITO, M., YAMANE, A.
  • Journal Title: Jpn. J. Gerodontol. 22 Pages: 280-287
  • NAID: 10025742851
  • Peer Reviewed
• [Journal Article] Characteristics of masticatory and tongue muscles (2007)
  • Author(s): YAMANE, A., FUKUI. T.
  • Journal Title: J. Oral Biosci. 49 Pages: 206-210
  • NAID: 10027102855
  • Peer Reviewed
• [Journal Article] 舌筋、咀嚼筋の発生、発達、再生 (2007)
  • Author(s): 山根 明、天野 修
  • Journal Title: 口腔組織培養学会誌 16 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALDER, C., DANIELL, J., GALANG, T.M., FUKUI, T., YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH,T.G.H.
  • Journal Title: J. Histochem. Cytochem. 55・2 Pages: 127-140
• [Journal Article] Transforming growth factor・s are up-regulated in the rat masseter muscle hypertrophied by clenbuterol, a・2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SIIIMADA, A. and YAMANE, A.
  • Journal Title: Brit J. Pharmacol 147(4) Pages: 412-421
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SIIIMADA, A and YAMANE, A.
  • Journal Title: Archs. oral Biol. 51(7) Pages: 603-611
• [Journal Article] Transforming growth factor βs are up-regulated in the rat masseter muscle hypertrophied by clenbuterol, a β_2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SHIMADA, A, YAMANE, A.
  • Journal Title: Brit J. Pharmacol. 147・4 Pages: 412-421
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SHIMADA, A, YAMANE,A.
  • Journal Title: Archs. oral Biol. 51・7 Pages: 603-611
• [Journal Article] Contribution of occlusal activity on synaptogenesis in masticatory muscles
  • Author(s): KOTA, Y., YAMANE, A., TOMOIIIRO, T. and ASADA, Y.
  • Journal Title: J. Dent. Res. (In press)
• [Journal Article] TGF・3 is expressed in differentiating muscle of the embryonic mouse tongue
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R. and MORITO, M.
  • Journal Title: Int. J. Dev. Biol. (In press)
• [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R., MORITO. M.
  • Journal Title: Int. J. Dev. Biol. (In press)
  • Peer Reviewed
• [Journal Article] Contribution of occlusal activity on synaptogenesis in masticatory muscles
  • Author(s): KOUTA, Y., YAMANE, A., TOMOHIRO, T.
  • Journal Title: J. Dent. Res. (In press)
  • Peer Reviewed
• [Journal Article] Treatment with invisible multiloop appliance of a horizontal open bite female young adult patient with tooth trauma
  • Author(s): FUKUI, T.
  • Journal Title: Am. J. Orthod. Dentofac. Orthop. (In press)
  • Peer Reviewed
• [Journal Article] Treatment with invisible multiloop appliance of a horizontal open bite female young adult patient with tooth trauma
  • Author(s): FUKUI, T.
  • Journal Title: American Journal of Orthodontics Dentofacial Orthopedics (In press)
  • Peer Reviewed
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M, YAMANE,A.
  • Journal Title: Cell and Tissue Res. (In press)
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. (In press)
• [Presentation] 器官培養系を用いたマウス舌形態形成の研究 (2009)
  • Author(s): 山根明、福井只美
  • Organizer: 生体運動合同班会議
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2009-01-11
• [Presentation] 分化過程のマウス舌筋細胞におけるTGFβおよびその受容体、シグナル伝達因子の発現 (2008)
  • Author(s): 山根明、福井只美
  • Organizer: 第50回歯科基礎医学会学術大会・総会
  • Place of Presentation: 有明コンベンションセンター
  • Year and Date: 2008-09-25
• [Presentation] 分化過程のマウス舌筋細胞におけるTGFβおよびその受容体、シグナル伝達因子の発現 (2008)
  • Author(s): 山根 明, 福井只美
  • Organizer: 第50回歯利基礎医学会学術大会・総会
  • Place of Presentation: 有明コンベンションセンター
  • Year and Date: 2008-09-25
• [Presentation] BMP2はTIMPとMMPの発現を調節することによりマウス舌側溝形成を制御している (2008)
  • Author(s): 福井只美, 山根 明
  • Organizer: 第67回日本矯正歯科学会大会
  • Place of Presentation: 幕張メッセ
• [Presentation] トランスフォーミング増殖因子βはマウス舌筋分化調節過程に関与している (2007)
  • Author(s): 山根明、福井只美、菅武雄、篠原暁子、森戸光彦
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-22
• [Presentation] 活発に分化しているマウス舌筋組織にBMPおよびその受容体が局在している (2007)
  • Author(s): 菅武雄、山根明、森戸光彦
  • Organizer: 第18回日本老年歯科医学会総会学術大会
  • Place of Presentation: 札幌市教育文化会館
  • Year and Date: 2007-06-22
• [Presentation] BMP2 functions as a negative regulator for the differentiation of muscle, but not as an inducer for cartilage and bone in the embryonic development of mouse tongue Gordon Research Conference "Myogenesis" (2007)
  • Author(s): YAMANE, A.
  • Organizer: Il Ciocco
  • Place of Presentation: Italy
  • Year and Date: 2007-05-16
• [Presentation] BMP2はマウス舌筋芽細胞分化において負の制御因子として機能している (2007)
  • Author(s): 山根明、福井只美、菅武雄、篠原暁子
  • Organizer: 第80回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2007-03-16
• [Presentation] BMP2 inhibits the differentiation of tongue myoblasts (2006)
  • Author(s): YAMANE, A., NAGATA, J., FUKUI, T. and SUGA, T.
  • Organizer: 14th Conference of the international Society of Differentiation
  • Place of Presentation: Congress Innsbruck
  • Year and Date: 2006-10-08
• [Presentation] Roles of BMP in the differentiation of tongue myoblasts (2006)
  • Author(s): YAMANE, A. NAGATA, J., FUKUI, T. and IIIRASIIITA, A.
  • Organizer: 第40回日本発生生物学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2006-06-02
• [Book] スタディ歯科薬理学 (2009)
  • Author(s): 山根明著
  • Total Pages: 216
  • Publisher: 永末書店
• [Book] スタディ歯科薬理学 (2009)
  • Author(s): 山根 明
  • Total Pages: 260
  • Publisher: 永末書店