| Budget Amount *help |
¥4,430,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2006: ¥400,000 (Direct Cost: ¥400,000)
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| Research Abstract |
(1) Histological findings for the tooth germ development
The mandibles obtained from the EL/kw (control mouse) and EL/sea (mutant mouse) mice at 5, 7.10 days of age were in Bouin's fixtative for 24h, decalcified in 14% EDTA for 1 week, dehydrated, and then embedded in paraffin. The section were stained with hematoxylin and eosin, and observed under a light microscope. The development of the 3^<rd> morlar (M3) tooth germ of EL/sea was slower than EL/kw at 5 days of age. However, tooth crown morphogenesis was progressing normally. The malformation of the M3 tooth germ was found on 7 days of age and arrest of M3 tooth germ development was observed in EL/sea. When it was the ten days after birth, the tooth germ almost disappeared in EL/sea, compared with normal development in EL/kw.
(2) Immunohistochemistry for the M3
The tissues were fixed in 4% paraformaldehyde at 4℃ overnight, and decalcified in 12.5% EDTA. They were dehydrated, embedded in paraffin. In EL/kw of 5, 7 and 10 days of age. Lef 1 was expressed in a tooth germ of M3 and the surrounding cells. On the other hand, the expression of Lef 1 was varied by 5, 7 and 10 days of age in EL/sea mice. In 7-day-old EL/sea mice, strong Lef 1 expression was detected in the tooth germ of M3. In both 5 and 10 days of age, Lef 1 expression was down regulated almost completely. In both EL/kw and EL/sea mice of 5, 7 and 10 days of age, there were observed no significant differences in the expression of EGF. From the result, it was suggested that Lef 1 may play an important role in arrest of the germ development of M3.
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