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Development of rapid analysis device for periodontal disease activity

Research Project

Project/Area Number 18592262
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KATAOKA Masatoshi  National Institute of Advanced Industrial Science and Technology, National Institute of Advanced Industrial Science and Technology, Health Technology Research Center, Team Leader (20224438)

Co-Investigator(Kenkyū-buntansha) KIDO Jun-ichi  Tokushima University, Department of Periodontology and Endodontology, Oral and Maxillofacial Dentistry, Associate Professor (10195315)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsDisease activity of periodontitis / Biomarker / Biochip / immunoreaction / multianalysis / 歯周病 / マイクロチップ電気泳動 / 診断デバイス / 疾患活動性 / PICP
Research Abstract

Several biomarkers including procollagen type I C-terminal propeptide(PICP), have been reported for objective evaluation of the state of periodontal disease. Although these markers are usually examined by conventional ELISA method with a microplate reader, it is time-and sample-consuming, and it is not adaptable to automated analyzers. Convenient, sensitive, and accurate methods for the measurement of the biomarkers are thus required. Bio-nano technology has received considerable interest in analytical chemistry due to the intrinsic characteristics of high speed, high throughput, easy operation, low consumption of samples and reagents, miniaturization, and automation. To develop the analysis system of biomarkers in periodontal disease in dental clinic, we first developed the quantitative analysis system based on the antigen-antibody reaction in microchannel on microchip. After the adsorption of 1st anti-PICP antibody on the surface of microchannel (300 μm width, 100 μm depth) on cyclic olefin copolymers (COC) microchip, sample and peroxidase-conjugated 2nd anti-PICP antibody mixture was introduced into a microchannel for 30 min, followed by washing and peroxidase activity was measured for the determination of PICP level. A double-logarithm plot analysis revealed a linear relationship between PICP concentration and peroxidase activity in the range of 50 to 600 ng/ml of PICP (r^2=0.9745). PICP concentrations in humoris of four subjects determined by the present methods were compared with the results obtained by conventional ELISA method. Good correlations were observed for each method on simple linear regression analysis (P<0.05). We demonstrated the possible application of microchip for determination of PICP levels with high sensitivity and accuracy, and the potential for the dental clinical diagnosis.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2008 2007 2006 Other

All Journal Article (21 results) (of which Peer Reviewed: 12 results) Presentation (3 results) Book (2 results)

  • [Journal Article] Accurate quantitation of salivary and pancreatic amylase activities in human plasma by microchip electrophoretic separation of the substance and hydrolysates coupled with immunoinhibition.2008

    • Author(s)
      Maeda E, et. al.
    • Journal Title

      Electrophoresis 28

      Pages: 1902-1909

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli,wheat germ,and rabbit reticulocytes.2008

    • Author(s)
      Hino M, et. al.
    • Journal Title

      J Biotechnol 133

      Pages: 183-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functionally important conserved length of C-terminal regions of yeast And bovine ADP/ATP carriers,identified by deletion mutants studies,and water accessibility of the amino acids at the C-terminal region of the yeast carrier.2008

    • Author(s)
      Iwahashi A, et. al.
    • Journal Title

      Mitochondrion 8

      Pages: 196-204

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Importance of probe location for quantitative comparison of signal intensities among genes in microarray analysis.2008

    • Author(s)
      Kakuhata R, et. al.
    • Journal Title

      J Biochem Biophys Methods 70

      Pages: 926-931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experimental animals.2008

    • Author(s)
      Watanabe M, et. al.
    • Journal Title

      Biochem Biophis Acta 1777

      Pages: 104-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Accurate quantitation of salivary and pancreatic amylase activities in human plasma by microchip lectrophoretic separation of the substance and hydrolysates coupled with immunoinhibition.2008

    • Author(s)
      Maeda E., et. al.
    • Journal Title

      Electrophoresis 28

      Pages: 1902-10909

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes.2008

    • Author(s)
      Hino M., et. al.
    • Journal Title

      J Biotechnol 133

      Pages: 183-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functionally important conserved length of C- terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier.2008

    • Author(s)
      Iwahashi A., et. al.
    • Journal Title

      Mitochondrion 8

      Pages: 196-204

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Importance of probe location for quantitative comparison of signal intensities among genes in microarray analysis.2008

    • Author(s)
      Kakuhata R., et. al.
    • Journal Title

      J Biochem Biophys Methods 70

      Pages: 926-931

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experimental animals.2008

    • Author(s)
      Watanabe M., et. al.
    • Journal Title

      Biochem Biophis Acta 1777

      Pages: 104-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Efficiency of cell-free protein synthesis based on a crude cen extract from Escherichia coli,wheat grem,and rabbit reticulocytes2008

    • Author(s)
      Hino Mami, et. al.
    • Journal Title

      J Biotechnol 133

      Pages: 183-189

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functionally important conserved length of C-terminal regions ofyeast and bovine ADP/ATP carriers,identified by deletion mutants studies,and water accessibihty ofthe amino acids at the C-terminal region ofthe yeast canier2008

    • Author(s)
      Iwahashi Akihiro
    • Journal Title

      Mitochondrion 8

      Pages: 196-204

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Importance of probe location for quantitative compalison bf signal intensities among genes in microarray analysis2008

    • Author(s)
      Kakuhata Rei, et. al.
    • Journal Title

      J Biochem Biophys Methods 70

      Pages: 926-931

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experhnental animals2008

    • Author(s)
      Watanabe Masahiro, et. al.
    • Journal Title

      Biochim Biophys Acta 1777

      Pages: 104-112

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free detection of protein-protein interaction at the GaAs/Water interface through surface infrared spectroscopy:Discrimination between specific and non-specific interactions by using secondary structure analysis.2007

    • Author(s)
      Hirano-Iwata, et. al.
    • Journal Title

      Langmuir 23

      Pages: 12287-12292

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Label-free detection of protein-protein interaction at the GaAs/Water interface through surface infrared spectroscopy : Discrimination between specific and non-specific interactions by using secondary structure analysis.2007

    • Author(s)
      Hirano-Iwata A., et. al.
    • Journal Title

      Langmuir 23

      Pages: 12287-12292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Label-free detection ofprotein-protein interactions at the GaAs/Water interface through surface infrared spectroscopy:Discrimination between specific and non-specific interactions by using secondaly stmcture analysis2007

    • Author(s)
      Hirano-Iwata Ayumi, et. al.
    • Journal Title

      Langmuir 23

      Pages: 12287-12292

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Norepinephrine stimulates calprotectin expression in human monocyticcells2006

    • Author(s)
      Suryono
    • Journal Title

      J Periodontal Res 41・3

      Pages: 159-164

    • Related Report
      2006 Annual Research Report
  • [Journal Article] High turnover osteoporosis is induced by cyclosporin A in rats2006

    • Author(s)
      Chic Wada
    • Journal Title

      J Bone Miner Metab 24・3

      Pages: 199-205

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Accurate quantitation of salivary and pancreatic amylase activities in human plasma by microchip electrophoretic separation of the substrates and hydrolysates coupled with immunoinh ibition

    • Author(s)
      Maeda Eiki, et. al.
    • Journal Title

      Electrophoresis (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of human blood glucose lebels using microchip electrophoresis

    • Author(s)
      Eiki Maeda
    • Journal Title

      Electrophoresis (in press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] Design preparation and directional insertion of peptides into lipid bilayer membrane and their application for the preparation of liposome of which surface could be coated by externally antibody.2007

    • Author(s)
      Matsuo T, et. al.
    • Organizer
      2007 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya,Japan
    • Year and Date
      2007-11-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Design preparation and directional insertion of peptides into lipid bilayer membrane and their application for the preparation of liposome of which surface could be coated by externally antibody.2007

    • Author(s)
      Matsuo T., et. al.
    • Organizer
      2007 International Symposium on Micro-NanoMechatronics and Human Sciece
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2007-11-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Design,preparation and directional insertion of peptides into lipid bilayer membrane and their apphcation for the preparation of liposome of which surface couldbe coated by externally added antibody2007

    • Author(s)
      Matsuo Taisuke, et. al.
    • Organizer
      2007 International Symposium on Micro-NanoMec-hatronics alld Human Science MHS2007
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2007-11-12
    • Related Report
      2007 Annual Research Report
  • [Book] バイオセンサ・ケミカルセンサ辞典、第III編第2章第2節キャピラリー型DNA検知システム2007

    • Author(s)
      片岡正俊、篠原康雄
    • Total Pages
      8
    • Publisher
      テクノシステム
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] バイオセンサ・ケミカルセンサ辞典、第III編第2章第2節キャピラリー型DNA検知システム2007

    • Author(s)
      片岡 正俊、篠原 康雄
    • Total Pages
      8
    • Publisher
      テクノシステム
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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