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Development of novel treatment for systemic infectious disease using salivary protein that inhibits bacterial

Research Project

Project/Area Number 18592282
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionKagoshima University

Principal Investigator

TAIHEI Yamaguchi  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (80230358)

Co-Investigator(Kenkyū-buntansha) OHO Takahiko  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (50160940)
MIZUEDANI Yukie  Kagoshima University, Medical and Dental Hospital, Assistant Professor (30305148)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsoral / normal colinizer / streptococci / adherence / aggregation / infection / opportunism / prevention / 応用微生物 / 感染症 / 細菌 / 生体分子 / 蛋白質 / 細菌感染 / 口腔細菌 / 唾液タンパク / アルブミン
Research Abstract

I. Objective : Human oral streptococci are associated with not only oral but systemic diseases. We have studied adherence activity of Streptococcus intermedius. Results suggested salivary factor inhibiting bacterial adherence. An application of such native factor of host could develop the oral flora in individuals. The approach could be creative and make health better. II. Methods and results : 1) Plastic well and immobilized actinomyces cells were coated with salivary samples derived from healthy individuals. Adherence activity of S. intermedius against these samples was measured. While many bacteria adhered to immobilized agglutinin, few bacteria did. The adherence was very different among individuals who had similar amount of salivary agglutinin also. 2) Whole saliva was fractionated by manipulation of a series of column. Wells were coated wit the mixture of purified salivary agglutinin and an aliquot of each fraction. The salivary factor inhibiting bacterial adherence was identifie … More d by adding labeled bacteria. The factor showed a molecule with approximately 60,000 as molecular weight. 3) The N-terminal amino acid sequence of the molecule was identified with that of human albumin. 4) The interaction of salivary agglutinin with high amount of human albumin indicated inhibition, but the reaction with low amount of albumin did enhancement. Two times more of bovine serum albumin was necessary to acquire the activity using human albumin. The recombinant human albumin derived from human liver lacking the 3rd to 6th exons demonstrated no effects containing inhibition and enhancement. 5) For inhibition and enhancement, albumin had to mix salivary agglutinin at the time of coating. Moreover, albumin did not affect bacterial aggregation in salivary agglutinin. III. Discussion : A part of oral streptococci adhere and aggregate associated with salivary agglutinin. This study demonstrated that salivary albumin worked as regulator for bacterial adherence. The results suggested that the bacterial adherence to host was regulated by multi factors. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (7 results)

All 2008 2007

All Journal Article (1 results) Presentation (6 results)

  • [Journal Article] Streptococcus intermediusの感染に関与する付着機構2008

    • Author(s)
      山口 泰平
    • Journal Title

      鹿児島大学歯学部紀要 28

      Pages: 27-38

    • NAID

      120001392932

    • Related Report
      2007 Annual Research Report
  • [Presentation] 口腔レンサ球菌の初期付着における唾液中の付着阻害因子の解析2008

    • Author(s)
      山口 泰平・於保 孝彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] 口腔レンサ球菌の口腔内付着を調節する唾液因子の解析2008

    • Author(s)
      山口 泰平、於保 孝彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analyses of salivary factor regulating adherence of oral streptococci2008

    • Author(s)
      Taihei, Yamaguchi, Takahiko, Oho
    • Organizer
      Japanese society for bacteriology
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 口腔レンサ球菌の口腔内付着を阻害する唾液因子の同定、解析2007

    • Author(s)
      山口 泰平・於保 孝彦
    • Organizer
      口腔衛生学会
    • Place of Presentation
      東京
    • Year and Date
      2007-10-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] 口腔レンサ球菌の口腔内付着を阻害する唾液因子の同定、解析2007

    • Author(s)
      山口 泰平、於保 孝彦
    • Organizer
      日本口腔衛生学会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and analyses of salivary factor inhibiting adherence of oral streptococci2007

    • Author(s)
      Taihei, Yamaguchi, Takahiko, Oho
    • Organizer
      Japanese society of dental health
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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