IgE-dependent TSLP production by human mast cells and the role of mast cell-derived TSLP in bronchial asthma
| Project/Area Number |
18604009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
アレルギー
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| Research Institution | Nihon University (2007)
The Institute of Physical and Chemical Research (2006) |
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Principal Investigator |
OKAYAMA Yoshimichi Nihon University, School of Medicine, Associate Professor (80292605)
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| Co-Investigator(Kenkyū-buntansha) |
SAGARA Hironori Dokkyo Medical University, Medical Department of University, Associate Professor (80275742)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,120,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Allergy / Bronchial asthma / mast cells / FcεRI / IgE / TSLP / 気管支喘息 / IL-4 / プロテアーゼ |
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| Research Abstract |
To investigate the thymic stromal lymphopoietin (TSLP) expression in asthmatic bronchial mucosa, we determined the cell populations expressing TSLP in the lungs of 16 asthmatics and of 11 controls by using immunohistochemistry. Ninety % of TSLP^+ cells were mast cells in the airways and the number of TSLP^+ mast cells was significantly increased in the airways of asthmatics (P < 0.0001). There was a significant correlation between the number of TSLP^+ mast cells and serum IgE level, hyperresponsibility, eosinophil count in the blood and the sputum, suggesting that mast cell-derived TSLP may play an important role in the pathogenesis of asthma. FcεRI-mediated activation of human mast cells upregulated TSLP mRNA expression 5.2±2.9 fold, and IL-4 significantly enhanced the FcεRI-mediated expression (53.7±15.9 fold, P< 0.05), but LPS and polyI : C failed to induce TSLP mRNA. We confirmed the production of TSLP in MC pellets and mast cell supernatants after activation in the presence of protease inhibitors, indicating mast cells are one of the main sources of TSLP in bronchial asthma.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Hyperexpression of FcγRI and Toll-like receptor 4 in the intestinal mast cells of Crohn's disease patients.in the intestinal mast cells of Crohn's disease patients2007
Author(s)
Kobayashi, R., Okamura, S., Ohno, T., Saito, H., Mori, M., Ra, C., Okayama, Y
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Journal Title
Clin Immunol 125(2)
Pages: 149-158
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Platelet-activating factor causes the activation of human mast cells2008
Author(s)
Kajiwara, N., Ohno, T., Oboki, K., Nakae, S., Sagara, H., Fukuda, T., Saito, H., Ra, C., Okayama, Y
Organizer
2008 American Academy of Allergy, Asthma and Immunology Annual Meeting
Place of Presentation
Philadelphia, Pensilvenia
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Mast cell (MC)-derived thymic stromal lymphopoietin (TSLP) activates NKT cells to produce IL-4 in asthmatic airways2008
Author(s)
Okayama, Y., Sagara, H., Okumura, S., Watanabe, N., Fueki, M., Sugiyama, K., Takeda, K., Fukuda, T., Saito, H., Ra, C
Organizer
2008 American Academy of Allergy, Asthma and Immunology Annual Meeting
Place of Presentation
Philadelphia, Pensilvenia
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] IL-4 upregulates FcεRI-mediated TSLP expression by human mast cells2007
Author(s)
Okayama, Y., Sagara, H., Okumura, S., Yuki, K., Fukuda, T., Saito, H
Organizer
2007 American Academy of Allergy, Asthma and Immunology Annual Meeting
Place of Presentation
San Diego.California
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Increase of amphiregulin level in sputum during exacerbation of acute asthma attacks in children2007
Author(s)
Enomoto, Y., Takamasu, T., Nakazawa, M., Kurihara, K., Minami, M., Saito, H., Okayama, Y
Organizer
2007 American Academy of Allergy, Asthma and Immunology Annual Meeting
Place of Presentation
San Diego, California
Description
「研究成果報告書概要(欧文)」より
Related Report
