Molecular Mechanism of heat-shock response and high-temperature adaptation in psychrophilic bacteria

• Project/Area Number: 18608003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 極限環境生物学
• Research Institution: Ehime University
• Principal Investigator: HAYASHI Hidenori Ehime University, 無細胞生命科学工学研究センター, 教授 (60124682)
• Co-Investigator(Kenkyū-buntansha): 奥山 英登志 北海道大学, 地球環境科学研究科, 准教授 (90125295) ; 西山 佳孝 愛媛大学, 無細胞生命科学工学研究センター, 准教授 (30281588)
• Co-Investigator(Renkei-kenkyūsha): OKUYAMA Hidetoshi 北海道大学, 地球環境科学研究科, 准教授 (90125295) ; NISHIYAMA Yoshitaka 愛媛大学, 無細胞生命科学工学研究センター, 准教授 (30281588)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 好冷性細菌 / 遺伝子発現 / 蛋白質 / 熱ショック応答 / 高温適応 / 微生物 / 生理活性

Research Abstract

好冷性生物は、通常の温度域で生育する生物が低温ストレスと感じる温度を高温ストレスと感じ、これに応答する。即ち高温ストレスにもっとも鋭敏に応答する生物であると考えることができ、その応答機構には他の生物とは異なる特徴があることが期待される。本研究ではゲノム情報を利用して、好冷性微生物における高温ストレス応答の普遍性と特異性、熱ショックタンパク質の構造的特徴と機能に関する知見を得た。

Research Products

• [Journal Article] pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis FEMS Microbiol (2009)
  • Author(s): Yoshitake Orikasa, Mika Tanaka, Shinji Sugihara, Ryuji Hori, Takanori Nishida, Akio Ueno Naoki Morita, Yutaka Yano, Kouhei Yamamoto, Akira Shibahara, Hidenori Hayashi, YohkoYamada, Akiko Yamada, Reiko Yu, Kazuo Watanabe9, & Hidetoshi Okuyama
  • Journal Title: Lett. (in press)
  • Peer Reviewed
• [Journal Article] pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis (2009)
  • Author(s): Y Orikasa, M Tanaka, S Sugihara, R Hori, T Nishida, A Ueno, N Morita, Y Yano, K Yamamoto, A Shibahara, H Hayashi, Y Yamada, A Yamada, R Yu, K Watanabe, H Okuyama
  • Journal Title: FEMS Microbiol. Lett. (印刷中)
  • NAID: 120002128711
  • Peer Reviewed
• [Journal Article] Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium (2007)
  • Author(s): Kanesaki Y, Yamamoto H, Paithoonrangsarid K, Shoumskaya M, Suzuki I, Hayashi H, Murata N.
  • Journal Title: Synechocystis sp. PCC 6803. Plant J. 49(2) Pages: 313-24
  • Peer Reviewed
• [Journal Article] Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II (2007)
  • Author(s): K. Kojima, M. Oshita, Y. Nanjo, K. Kasai, Y. Tozawa, H. Hayashi and Y. Nishiyama
  • Journal Title: Molecular Microbiology 65 Pages: 936-947
  • Peer Reviewed
• [Journal Article] Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803 (2007)
  • Author(s): Kanesaki Y, Yamamoto H, Paithoonrangsarid K, Shoumskaya M, Suzuki I, Hayashi H, Murata N.
  • Journal Title: Plant Journal 49 Pages: 313-324
  • Peer Reviewed
• [Journal Article] Oxidation of elongation factor G inhibits the synthesis of the D 1 protein of photosystem II (2007)
  • Author(s): K. Kojima, M. Oshita, Y. Nanjo, K. Kasai, Y. Tozawa, H. Hayashi and Y. Nishiyama
  • Journal Title: Molecular Microbiology 65 Pages: 936-947
  • Peer Reviewed
• [Journal Article] Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803. (2007)
  • Author(s): Kanesaki Y, Yamamoto H, Paithoonrangsarid K, Shoumskaya M, Suzuki I, Hayashi H, Murata N.
  • Journal Title: Plant J. 49(2) Pages: 313-324
• [Journal Article] The rpoH gene encoding heat shock sigma factor sigma32 of psychrophilic bacterium Colwellia maris (2006)
  • Author(s): Yamauchi S, Okuyama H, Nishiyama Y, Hayashi H
  • Journal Title: Extremophiles 10 Pages: 149-58
  • NAID: 120000955292
  • Peer Reviewed
• [Journal Article] The rpoH gene encoding heat shock sigma factor σ^32 of psychrophilic bacterium Colwellia maris. (2006)
  • Author(s): Yamauchi S, Okuyama H, Nishiyama Y, Hayashi H.
  • Journal Title: Extremophiles 10(2) Pages: 146-158
• [Journal Article] Acclimation of photosystem II to high temperature in a suspension culture of soybean (Glycine max) cells requires proteins that are associated with the thylakoid membrane. (2006)
  • Author(s): Nishiyama Y, Takechi K, Nanjo Y, Murata N, Hayashi H.
  • Journal Title: Photosynth Res. 90(3) Pages: 223-232
• [Presentation] 好冷性細菌Colwellia psychrerythraea 34Hが持つ2種類の熱ショックタンパク質DnaKの機能解析 (2008)
  • Author(s): 深田伸介、納山かおり、山内清司、杉浦美和、林秀則
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 東京
• [Presentation] 好冷性細菌Colwellia psychrerythraea strain 34HのシャペロニンGroELの機能解析 (2008)
  • Author(s): 山内清司、杉浦美羽、林秀則
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 東京
• [Presentation] ラン藻の光化学系IIの高温適応に関与する脂肪酸合成酵素 (2008)
  • Author(s): 南條洋平、西山佳孝、林秀則
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] 好冷性細菌の熱ショックタンパク質発現機構の解析 (2006)
  • Author(s): 山内清司、奥山英登志、西山佳孝、林秀則
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 東京
• [Presentation] Characteristics of rpoH Gene Encoding Heat Shock Sigma Factor s^<32> of Psychrophilic Bacterium Colwellia maris that Expresses Heat Shock Proteins at Relatively Low Temperature (2006)
  • Author(s): Hidenori Hayashi, Yoshitaka Nishiyama, Seiji Yamauchi and Hidetoshi Okuyama
  • Organizer: International Conference on Extremophiles
  • Place of Presentation: Brest, France